RESUMO
We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.
Assuntos
Neoplasias da Mama/metabolismo , Carcinoma Ductal de Mama/metabolismo , Perfilação da Expressão Gênica/métodos , Fixação de Tecidos/métodos , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/genética , Carcinoma Ductal de Mama/patologia , Feminino , Fixadores/química , Formaldeído/química , Expressão Gênica , Humanos , Pessoa de Meia-Idade , Estabilidade de RNA , Kit de Reagentes para DiagnósticoRESUMO
Authors analyzed and generalized experience of 2910 anaesthetic supports, performed at the Branch 6 of Vishnevskiy 3rd Central Military Clinical Hospital of Russian Federation Defense Ministry during 2010-2012 in case of outpatient surgery such as endoscopic examinations (gastrofibroscopy, colonoscopy), gynecological support, dental care, ORL-surgery, urologic surgery, diagnostic and treatment arthroscopy. Authors gave information about preoperative, intraoperative and postoperative aspects of outpatient anaesthesia.
