RESUMO
Back ground: Peri-conception care plays an essential and vital role for the health of the mother, her child and her family. Taking folic acid orally prior to conception and during the early stages of pregnancy plays a significant role in preventing Neural Tube Defects and has been associated with preventing other folic acid sensitive congenital anomalies. There exists problem in different parts of the globe on women's understanding of the importance of folic acid making them not to take this supplement in peri-conception period. Objective: This study aimed at assessing the knowledge of peri-conception folic acid supplementation among women visiting Maternal and Child Health clinics of public health facilities in Addis Ababa, Ethiopia. Methods: Institution based cross-sectional study was conducted from May 15 to June 20/2020 among 226 women visiting Maternal and Child Health clinics of health centers in Addis Ababa. Consecutive sampling technique was used to select study participants. Data were collected through interviewer administered structured questionnaire and analyzed using Statistical Package for Social Sciences version 24. Tables, charts and graphs were used for data presentation. Multivariable logistic regression analysis was done to identify the independent predictors of women's knowledge of folic acid at p-value ≤ 0.05. Results: More than two-third of the study participants, 156 (69.0%) had good knowledge on peri-conception folic acid supplementation and its health benefits. Participants with age 25-30 years were 2.14 times more likely to have good knowledge compared to those aged less than 25 years while women with age greater than 30 years were 5.20 times more likely to have good knowledge compared to those with age less than 25 years. Women who had attended high school were 2.93 times more likely to have good knowledge compared to those with no formal education. Likewise, women who had attended college and above were 4.18 times at odd of having good knowledge on periconception folic acid supplementation compared to those with no formal education. Women who had information on prenatal care were 1.48 times more likely to have good knowledge compared to those who never had no information. Conclusion: Folic acid supplementation knowledge in more than two third of the study participants was good. Participants' age, educational status and information on prenatal care were the independent predictors of knowledge on folic acid supplementation. The Ministry of health, Addis Ababa city health bureau, the study facilities' management as well as Non-Governmental Organizations working on health in the city should focus on means to increase knowledge of peri-conception folic acid health benefits for women with poor knowledge on the same.
RESUMO
BACKGROUND: The evolution of resistance to currently used malaria medicines together with the severe economic burden of malaria initiates the search for novel antimalarial drugs. Thus, the present experiment was intended to assess the in vivo antiplasmodial effect of Gardenia ternifolia based on the traditional claims and in vitro antimalarial effect of the plant. METHODS: For the crude extraction of stem barks of G. ternifolia, a cold maceration method using hydromethanol as a solvent was employed. The hydroalcoholic extract was then fractionated by three solvents (chloroform, n-butanol, and aqueous solvent) with different polarity indexes. Swiss albino mice infected with the chloroquine-sensitive malaria parasite (Plasmodium berghei) were used in this study. Acute oral toxicity study was done according to standard protocols. Four-day suppressive (hydromethanolic crude extract and solvent fractions), Rane's (crude extract), and repository (crude extract) tests were used to examine the antiplasmodial effects of the study plant. RESULTS: The chemosuppressive study revealed that all doses of the crude extract and its fractions displayed a significant (P < 0.001) inhibition of parasitemia compared with the vehicle (negative control). The crude extract's highest dose (600 mg/kg) showed the maximum (57.84%) parasitemia suppression during the chemosuppressive test. The crude extract also produced significant (P < 0.001) curative and prophylactic effects at all doses in Rane's and repository tests compared with the negative control. In the 4-day test, the n-butanol fraction produced parasitemia suppression higher than the chloroform fraction but lower than the crude extract. Of these, water fractions demonstrated the lowest chemosuppressive effect. Anthraquinone, alkaloids, flavonoids, saponins, steroids, tannins, and terpenoids were qualitatively detected in the plant material. CONCLUSION: The current results showed that the hydromethanolic extract and fractions of G. ternifolia stem barks have antiplasmodial action with a high curative effect. Chloroform and n-butanol fractions were more active among the fractions, indicating that the nonpolar and semipolar constituents of the plant are responsible for the antimalarial effects.
