[SAFETY AND IMMUNOGENICITY OF A NATIONAL COMBINED VACCINE AGAINST PERTUSSIS, DIPHTHERIA, TETANUS, HEPATITIS B AND Hib-INFECTION, CONTAINING ACELLULAR PERTUSSIS COMPONENT, DURING IMMUNIZATION OF ADULTS].

AIM: Study safety, reactogenicity and immunologic effectiveness of a national combined vaccine against diphtheria, pertussis (acellular component), tetanus, hepatitis B and Hib-infection during immunization of volunteers aged 18-60 years. MATERIALS AND METHODS: The study was carried out in accordance with ethical standards and requirements, regulated by Helsinki declaration and Good clinical practice (ICHGCP). In a simple non-randomized clinical trial 20 adult volunteers took part, the mean age of those was 46.9 years. RESULTS: Registered: post-vaccination reactions (both local and systemic) were mild and of moderate degree of severity, stopped independently after 2-3 days without administration of drug treatment. Postvaccinal complications were not noted. Parameters of general and biochemical analysis of blood, urine, IgE content in dynamics of immunization were within normal limits. A single administration of aAPDT--HepB+Hib to individuals aged 18-60 years resulted in development of antibodies against all the components of the preparation. Seroconversion factor fluctuated from 6.9 to 53.5: CONCLUSION: The results obtained allow to recommend the vaccine for evaluation of its safety, reactogenicity, immunologic and prophylaxis effectiveness in randomized clinical observation trials in children.

[ASSOCIATION OF CERTAIN SINGLE NUCLEOTIDE POLYMORPHISM GENES OF APOPTOSIS SYSTEM WITH A RISK OF DEVELOPMENT OF COLORECTAL CANCER IN RUSSIAN POPULATION].

AIM: Study the effect of single nucleotide polymorphism genes TP53 (rs1042522, rs1800371), CDKN2A (rs3731217, rs3088440) and MDM2 (rs2279744) on the risk of development of color- ectal cancer (CRC) in population of Perm Region. MATERIALS AND METHODS: Case group con- sisted of 198 patients with histologically verified CRC, control group - 205 individuals with CRC excluded by results of colonoscopy. DNA genotyping, obtained from leukocytes of venous blood ofthe studied individuals; was carried out by PCRwith electrophoretic detection of results. RESULTS: Significant inter-population differences of frequency of occurrence of alleles rs 1042522; rs3088440, rs2279744 in Russian population compared with East-Asian and European were detected (p<0.0001). Association of heterozygote (G/T) genotype rs2279744 with a lower risk of development of CRC regardless of sex and age (OR=0.51, 95% CI=0.26 - 0.97) was established. Statistically significant relations between development of CRC and other polymorphisms were not determined. CONCLUSION: Relations of gene polymorphism of apoptosis system with risk of development of CRC in Russian population was studied for the first time. The data obtained give evidence on the probable reduction of risk of development of CRC with carriage of hetero- zygote genotype of polymorphism rs2279744.

[Epidemiological characteristics of malignant tumor incidence of the population of the Perm region].

The article presents the results of an epidemiological descriptive and retrospective study of the malignant tumor incidence in the Perm region according to official statistics for the 2002-2014. Through the use of standardized rates it was revealed that the incidence of growth mainly due to an increased risk of illness because of the intensification of epidemiological factors (13,1%). Changes in the total extent and structure of population resulted in growth of number of cases by 3,5% of the total increase. Over 80% of the total incidence was recorded among persons older than 50 years. Cancer of the breast, skin, colon and rectum and lung were characterized by the greatest intensity of oncoepidemiological process. The highest growth rate of incidence had ovarian cancer, cervical cancer, endometrial cancer and prostate cancer.

[The association between diet and the probability of colorectal cancer among the population of Perm krai: epidemiological study].

Colorectal cancer (CRC) is one of the main causes of morbidity and mortality among all malignant tumors both in the world and in Russia. The purpose of the research was to study the association between diet and the probability of CRC in Perm Krai. The epidemiological analytical case-control study was performed. The questionnaire survey included 191 histologically proved colorectal cancer cases and 200 healthy individuals with excluded CRC by the results of colonoscopy. The surveyed groups were spread evenly by sex, age, ethnicity, place of residence and smoking (p>0.05). The odds to determine the following factors were higher in case group: diet including fried foods (OR=2.45, 95% CI: 1.58-3.80, p<0.0001), bread more 100 g per day (OR=1.72, 95% CI: 1.72-2.60, р=0.005), over-salted food (OR=1.97, 95% CI: 1.27-3.04, p=0.001), consumption of spicy foods (OR=2.82, 95% CI: 1.59-5.13, p<0.0001) and dairy products less than 500 g per week (OR=3.94, 95% CI: 2.60-5.97, p<0.0001). The odds to determine the following factors were higher in control group: an exclusion of processed (OR=0.45, 95% CI: 0.28-0.70, p<0.0001) and red meat (OR=0.19, 95% CI: 0.05-0.51, p<0.0001). Consequently, at the present study the diet factors that have a higher rate in group of CRC patient's compared to healthy individuals have been determined. This requires to study interaction between diet, another environmental and genetic factors.

[EXPERIENCE OF USING IMMUNE CHROMATOGRAPHY TEST FOR DIAGNOSTICS OF PNEUMOCOCCAL PNEUMONIA].

AIM: Justification of the use of immune chromatographic analysis for diagnostic of pneumococcal pneumonia. MATERIALS AND METHODS: Sensitivity and specificity of an immune chromatographic method (Binax Now test system) was studied for verification of pneumococcal pneumonia. Approbation of this method for etiologic deciphering of pneumonia in 260 patients hospitalized in infectious hospital was carried out. RESULTS: A high sensitivity (84.8%) and specificity (90.5%) of the chromatography test was established. Streptococcus pneumoniae antigen in urine of patients with community acquired pneumonia was determined 3.6 times more frequently when immune chromatographic test was used compared with bacteriological study of nasopharyngeal swab and 1.8 times more frequently compared with sputum bacteriological study. CONCLUSION: Application of immune chromatographic express test Binax Now could be recommended for timely verification of pneumococcal pneumonia along with bacteriological methods of study.