The Double-Aspect of Life.

Life is based on two aspects: matter and a non-material, electrical component. In a dynamic system of reciprocal causality, matter and the so-called bioelectricity interact with one another, forming a functional unity. The aim of this essay is to summarize evidence for bioelectricity, for the sensitivity of biosystems to external physical factors and for the interactions of internal bioelectricity with internal biochemical structures. I propose non-material information of bioelectrical states to be just as inheritable from generation to generation as is the material genetic code.

Endogenous physical regulation of population density in the freshwater protozoan Paramecium caudatum.

Studies confirm physical long-range cell-cell communication, most evidently based on electromagnetic fields. Effects concern induction or inhibition of cell growth. Their natural function is unclear. With the protozoan Paramecium caudatum I tested whether the signals regulate cell density and are electromagnetic. Up to 300 cells/mL, cell growth in clones of this study is decreasingly pronounced. Using cuvettes as chemical barriers enabling physical communication  I  placed 5 indicator cells/mL, the inducer populations, into smaller cuvettes that stand in bigger and contained 50, 100, 200 or 300 cells/mL. Under conditions of total darkness such pairs were mutually exposed for 48 hours. The hypothesis was that indicator cells, too, grow less the more neighbor cells there are. The bigger inducer populations were in the beginning the less they grew. The indicator populations grew accordingly; the more cells they were surrounded by the less they grew. The suppressing neighbors-effect disappeared when inner cuvettes were shielded by graphite known to shield electromagnetic radiation from GHz to PHz, i.e. to absorb energy from microwaves to light. These are the first results demonstrating non-contact physical quorum sensing for cell population density regulation. I assume rules intrinsic to electromagnetic fields interacting with matter and life.

Physical Non-Contact Communication between Microscopic Aquatic Species: Novel Experimental Evidences for an Interspecies Information Exchange.

Previous experiments on physical non-contact communication within same species gave rise to test for this type of communication also across the species border, which was the aim of the present study. It was found that autotrophic unicellular organisms (Euglena viridis), separated by cuvettes, affected the proliferation rate of heterotrophic unicellular organisms (Paramecium caudatum). Further, the heterotrophic unicellular organism affected also the proliferation rate of a multicellular heterotrophic organism (Rotatoria sp.) and vice versa. In the case when populations (of Euglena viridis and Paramecium caudatum) were shielded against electromagnetic fields in the optical spectrum from each other, no effects were measured. The results may support the notion that the organisation of ecosystems relies also on the exchange of electromagnetic fields from their constituting biosystems.

Non-chemical and non-contact cell-to-cell communication: a short review.

Cell-to-cell communication is the basis of coordinated cellular activity and thus fundamental for the functioning of biological systems. In a recently published research article by Chaban et al. (Am. J. Transl. Res., 5(1), 69-79), the authors report on interesting new experimental findings supporting a neuro-hormonal independent, non-diffusible cell-to-cell signaling. Our paper aims to (i) discuss some critical notions used by the authors to describe their findings, and (ii) briefly review related experimental work performed so far but not discussed in the original work of Chaban et al. In our opinion, the research on principles of non-chemical and non-contact cell-to-cell communication has the potential to offer new fundamental insights into biological processes. With this paper, we want to encourage future research on this topic by discussing critical issues and giving an overview of the current state of research.

Cellular communication through light.

Information transfer is a fundamental of life. A few studies have reported that cells use photons (from an endogenous source) as information carriers. This study finds that cells can have an influence on other cells even when separated with a glass barrier, thereby disabling molecule diffusion through the cell-containing medium. As there is still very little known about the potential of photons for intercellular communication this study is designed to test for non-molecule-based triggering of two fundamental properties of life: cell division and energy uptake. The study was performed with a cellular organism, the ciliate Paramecium caudatum. Mutual exposure of cell populations occurred under conditions of darkness and separation with cuvettes (vials) allowing photon but not molecule transfer. The cell populations were separated either with glass allowing photon transmission from 340 nm to longer waves, or quartz being transmittable from 150 nm, i.e. from UV-light to longer waves. Even through glass, the cells affected cell division and energy uptake in neighboring cell populations. Depending on the cuvette material and the number of cells involved, these effects were positive or negative. Also, while paired populations with lower growth rates grew uncorrelated, growth of the better growing populations was correlated. As there were significant differences when separating the populations with glass or quartz, it is suggested that the cell populations use two (or more) frequencies for cellular information transfer, which influences at least energy uptake, cell division rate and growth correlation. Altogether the study strongly supports a cellular communication system, which is different from a molecule-receptor-based system and hints that photon-triggering is a fine tuning principle in cell chemistry.

Temperature-dependent transmission and latency of Holospora undulata, a micronucleus-specific parasite of the ciliate Paramecium caudatum.

Transmission of parasites to new hosts crucially depends on the timing of production of transmission stages and their capacity to start an infection. These parameters may be influenced by genetic factors, but also by the environment. We tested the effects of temperature and host genotype on infection probability and latency in experimental populations of the ciliate Paramecium caudatum, after exposure to infectious forms of its bacterial parasite Holospora undulata. Temperature had a significant effect on the expression of genetic variation for transmission and maintenance of infection. Overall, low temperature (10 degrees C) increased levels of (multiple) infection, but arrested parasite development; higher temperatures (23 and 30 degrees C) accelerated the onset of production of infectious forms, but limited transmission success. Viability of infectious forms declined rapidly at 23 and 30 degrees C, thereby narrowing the time window for transmission. Thus, environmental conditions can generate trade-offs between transmission relevant parameters and alter levels of multiple infection or parasite-mediated selection, which may affect evolutionary trajectories of parasite life history or virulence.