Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Fish Shellfish Immunol ; 88: 102-110, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30802631

RESUMO

Interleukin-17 (IL-17) is a proinflammatory cytokine that plays an important role in immune responses. In this study, we identified 57 IL-17 genes from the genomes of six marine invertebrates, including Pinctada fucata martensii, Crassostrea gigas, Lottia gigantea, Capitella teleta, Mizuhopecten yessoensis, and Mytilus galloprovincialis. Phylogenetic analysis showed that all invertebrate IL-17 genes were clustered into one group, implying that invertebrate IL-17 evolved from one common ancestral gene. From the extron-intron analysis, we found many intronless IL-17 genes in mollusks, which may be caused by retroposition. Tissue and development transcriptomic analysis showed that the expression of PmIL-17 was tissue and developmental stage-specific. Moreover, we cloned the full length of the IL-17-2 gene from P. f. martensii (PmIL-17-2) and explored its function in the immune response. The full-length cDNA of PmIL-17-2 is 719 bp, containing an open reading frame of 564 bp, a 5' -untranslated region (UTR) of 31 bp, and a 3' -UTR of 124 bp with a 30 bp poly (A) tail. PmIL-17-2 had a strong response to lipopolysaccharide (LPS), indicating that the PmIL-17-2 participates in innate immune responses. In situ hybridization of hemocytes showed that PmIL-17-2 was mainly produced by granulosa cells, and the number of the stained granulosa increased after LPS stimulation. These results lay the foundation for the research of IL-17 family in marine invertebrates.


Assuntos
Evolução Biológica , Interleucina-17/genética , Pinctada/genética , Sequência de Aminoácidos , Animais , Bivalves/genética , Gastrópodes/genética , Perfilação da Expressão Gênica , Hemócitos/metabolismo , Humanos , Imunidade Inata/genética , Interleucina-17/metabolismo , Lipopolissacarídeos/farmacologia , Filogenia , Pinctada/crescimento & desenvolvimento , Pinctada/imunologia , Poliquetos/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...