Variation of Charge Carrier Dynamics for Polycrystalline Perovskite Films by One-Step Solution and Vapor-Deposition Methods.

To date, solution-processing and vapor-deposition fabrication methods have achieved huge successes in high-efficiency perovskite solar cells (PSCs) and satisfy special demanding requirements for diverse application purposes, respectively. Although people realize that the fabrication procedure is crucial in device performance, insightful studies of charge carrier dynamics in perovskite films by different methods still lack. In this work, we compare the carrier behaviors in one-step spin-coated and dual-source coevaporated MAPbI3 perovskite films by combining time-resolved photoluminescence spectroscopy and carrier dynamics simulation. We suggest that strains, lattice orientations, and defects at buried side of perovskite films, which are associated with different preparation processes, lead to variations in carrier behaviors. Hence fabrication of perovskite layers should be elaborately designed in order to satisfy the needs of different carrier behaviors in specified device configurations of PSCs such as smooth planar or textured monolithic tandem structures.

Nitric Oxide: A Neglected Driver for the Conjugative Transfer of Antibiotic Resistance Genes among Wastewater Microbiota.

The dissemination of plasmid-borne antibiotic resistance genes (ARGs) in wastewater is becoming an urgent concern. Previous studies mainly focused on the effects of coexisting contaminants on plasmid conjugation, but ignored the potential contribution of some byproducts inevitably released from wastewater treatment processes. Herein, we demonstrate for the first time that nitric oxide (NO), an intermediate of the wastewater nitrogen cycle, can significantly boost the conjugative transfer of plasmid RP4 from Escherichia coli K12 to different recipients (E. coli HB101, Salmonella typhimurium, and wastewater microbiota). Phenotypic and genotypic tests confirmed that NO-induced promotion was not attributed to the SOS response, a well-recognized driver for horizontal gene transfer. Instead, NO exposure increased the outer membrane permeability of both the donor and recipient by inhibiting the expression of key genes involved in lipopolysaccharide biosynthesis (such as waaJ), thereby lowering the membrane barrier for conjugation. On the other hand, NO exposure not only resulted in the accumulation of intracellular tryptophan but also triggered the deficiency of intracellular methionine, both of which were validated to play key roles in regulating the global regulatory genes (korA, korB, and trbA) of plasmid RP4, activating its encoding transfer apparatus (represented by trfAp and trbBp). Overall, our findings highlighted the risks of NO in spreading ARGs among wastewater microbiota and updated the regulation mechanism of plasmid conjugation.

Construction of Charge Transport Channels at the NiOx/Perovskite Interface through Moderate Dipoles toward Highly Efficient Inverted Solar Cells.

NiOx-based perovskite solar cells (PSCs) have attracted much attention because of their low fabrication temperature, suppressed hysteresis, and superior stability. However, the poor interfacial contacts between NiOx and perovskite layers always limit the progress of PSCs. Here, we applied 2-thiophenemethylamine (TPMA) as charge transport channels at the interface between NiOx and perovskite layers. The introduction of TPMA provides moderate dipole moment pointing to the perovskite side and effectively promotes the charge transportation. Meanwhile, TPMA anchorage also passivates the defect states at the surfaces of both NiOx and MAPbI3, which compensates the voltage loss due to the change in NiOx work function induced by the dipole. Thus, the device performance has been significantly enhanced in both electrochemical properties and power conversion efficiency. Our work has demonstrated a new way of improving current and voltage in the NiOx-based PSCs simultaneously through a moderate interfacial dipole moment toward highly efficient PSCs.

Effects of emerging pollutants on the occurrence and transfer of antibiotic resistance genes: A review.

The emergence and spread of antibiotic resistance genes (ARGs) have become major concerns for both public health and environmental ecosystems. Emerging pollutants (EPs) that accumulate in environmental compartments also pose a potential risk for the enrichment of ARGs in indigenous microorganisms. This paper presents a comprehensive review of the effects and intrinsic mechanisms of EPs, including microplastics, engineered nanomaterials, disinfection byproducts, pharmaceuticals, and personal care products, on the occurrence and dissemination of ARGs. State-of-the-art methods for identifying culture-independent ARG-host interactions and monitoring horizontal gene transfer (HGT) processes in real-time are first reviewed. The contributions of EPs to the abundance and diversity of ARGs are then summarized. Finally, we discussed the underlying mechanisms related to the regulation of HGT, increased mutagenesis, and the evolution of microbial communities. Further details of three HGT (i.e., conjugation, transformation, and transduction) frequency patterns in response to various EPs are also examined. This review contemplates and reassesses the risks of ARG evolution posed by the manufacture and application of EPs.

Effect of nano zinc oxide on the acute and reproductive toxicity of cadmium and lead to the marine copepod Tigriopus japonicus.

Given the absorbability of nZnO and its inevitable coexistence with trace metals such as Cd and Pb in coastal environment, nZnO can adsorb these pollutants thereby affecting their distribution in different media of marine ecosystem. The marine copepod Tigriopus japonicus was applied in the present study to investigate the combined effect of nZnO and Cd or Pb on mortality and reproduction in marine organisms. For acute exposure, presence of 1.0 mg/L nZnO increased the toxicity of both Cd and Pb, as their LC50 decreased from 5.9 and 75.4 mg/L to 3.95 and 48.0 mg/L, respectively. For 21 d chronic exposure, the reproduction of the copepod was influenced by Cd and Pb at environmental relevant concentrations, and the interaction between nZnO and Cd or Pb appeared to be antagonistic. The waterborne Cd and Pb concentration was affected by nZnO for neither acute nor chronic exposure, indicating no adsorption of these two metals to nZnO at relative low concentration. The overall findings of this study suggested the binary exposure to nZnO/Cd or nZnO/Pb might have potential different toxic mechanisms between acute and chronic exposure.