RESUMO
OBJECTIVE AND DESIGN: The study was aimed at screening out the mimetic peptides from the binding site of lipopolysaccharide binding protein and CD 14, and then observing if the mimetic peptide will inhibit in vitro LPS-induced inflammatory reaction and function as an anti-endotoxin in the model of LPS-induced acute lung injury. MATERIAL AND METHODS: Human monocytic cell line (U937) was used in vitro. Thirty three-month-old SD rats were used. Phage display peptide library was adapted to screen mimetic peptide sequences. TREATMENT: U937 cells were exposed to treatment with LPS and rhLBP and then were incubated with MP12 at three different concentrations after they were induced and differentiated by PMA. LPS intravenous injection was used to establish a model of rat acute lung injury which was later treated with intravenous injection of MP12. RESULTS: We successfully obtained the mimetic peptide of lipopolysaccharide-binding protein and CD 14 binding site, the gene sequence of which is FHRWPTWPLPSP (MP12). MP12 can markedly inhibit LPS induced TNF-alpha expression. MP12 can evidently increase PaO(2) of rats with acute lung injury and also increase the survival rate of these rats. CONCLUSIONS: MP12 (FHRWPTWPLPSP) has the same function as mimetic of lipopolysaccharide-binding protein and CD 14 binding site. The application of MP12, both in vitro and in vivo, confers the biological activity required to antagonise LBP/CD14 and block LPS inflammatory signals, and it can markedly enhance PaO(2) of rats suffering from acute lung injury and also enhance their survival rate.
Assuntos
Proteínas de Fase Aguda/metabolismo , Biomimética , Proteínas de Transporte/metabolismo , Receptores de Lipopolissacarídeos/metabolismo , Glicoproteínas de Membrana/metabolismo , Peptídeos/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Linhagem Celular , Humanos , Inflamação/imunologia , Inflamação/patologia , Receptores de Lipopolissacarídeos/genética , Lipopolissacarídeos/imunologia , Lipopolissacarídeos/toxicidade , Camundongos , Dados de Sequência Molecular , Monócitos/citologia , Monócitos/metabolismo , Biblioteca de Peptídeos , Peptídeos/genética , Ratos , Ratos Sprague-Dawley , Alinhamento de SequênciaRESUMO
According to the characteristics of anatomy and electrophysiology of vision, this paper describes the method of sampling along the visual path to obtain the integrated information of electrophysiology of vision. The developed system MET01 can detect and analyze EOG, ERG and VEP signals as a whole, and has a clinical value.
Assuntos
Potenciais Evocados Visuais/fisiologia , Estimulação Luminosa , Visão Ocular/fisiologia , Eletroculografia/métodos , Eletroculografia/normas , Eletrofisiologia/métodos , Eletrofisiologia/normas , Eletrorretinografia/métodos , Eletrorretinografia/normas , Humanos , Fotometria/normas , Transtornos da Visão/diagnóstico , Testes de Campo VisualRESUMO
Effects of Zuoguiwan (ZGW, a prescription for reinforcing Kidney Yin) on early embryonic development were observed by using embryonic developmental retardation model of mice formed by alcohol. Drug was given in three ways: add ZGW into cultural medium directly (group A), add the serum of mice received ZGW (group B) and cultured the embryo taken from ZGW treated mice (group C). The result was compared with that treated with Bazhen decoction (BZD, a prescription for supplementing Qi and blood). Results showed that the in vitro developmental rate of embryo from 2-cell stage to blastula stage in group B and C, which approached to normal control group, was higher than that in untreated model obviously. While in BZW group, it was higher than in normal control group only in certain stage. However, adding ZGW directly into culture medium didn't reveal marked effect on early embryonic development.
