SYAUP-CN-26 applies its antifungal activity against Botrytis cinerea by disrupting mitochondrial structure and function.

This study investigated the effect of SYAUP-CN-26 on mitochondrial structure and function of Botrytis cinerea. The mitochondria, with the addition of SYAUP-CN-26 (EC50 [1.823 mg/L], EC90 [19.263 mg/L], and minimum inhibitory concentration [MIC] [79.754 mg/L]), emerged malformed shape, rough surface and unordered structure. As the concentration of SYAUP-CN-26 increases, the decrease in ATP content and the enhancement in the inhibition of mitochondrial respiratory chain complexes function confirmed that mitochondrial function was disrupted. And the respiratory superposing inhibition showed that SYAUP-CN-26 inhibited the tricarboxylic acid cycle (TCA) pathway of B. cinerea cells. Overall, these results indicated that SYAUP-CN-26 could inhibit mitochondrial structure and function to effect the growth of B. cinerea cells, and inhibition of mitochondrial respiratory chain complexes was a key factor for disruption of B. cinerea mitochondrial function and antifungal activity.

The Effect of (1S,2R-((3-bromophenethyl)amino)-N-(4-chloro-2-trifluoromethylphenyl) cyclohexane-1 -sulfonamide) on Botrytis cinerea through the Membrane Damage Mechanism.

In recent years, Botrytis cinerea has led to serious yield losses because of its resistance to fungicides. Many sulfonamides with improved properties have been used. (1S,2R-((3-bromophenethyl)amino)-N-(4-chloro-2-trifluoromethylphenyl)cyclohexane-1-sulfonamide) (abbreviation: SYAUP-CN-26) is a new sulfonamide compound that has excellent activity against B. cinerea. This study investigated the effect of SYAUP-CN-26 on electric conductivity, nucleic acids leakage, malondialdehyde (MDA) content, and reducing sugars and membrane structure reduction of B. cinerea. The results showed that the cell membrane permeability of B. cinerea increased with increasing concentrations of SYAUP-CN-26; meanwhile, the sugar content decreased, the malondialdehyde content increased, and relative electric conductivity and nucleic acid substance leakage were observed in the cell after exposure to 19.263 mg/L SYAUP-CN-26 for 24 h. After 48 h of exposure to 1.823 mg/L and 19.263 mg/L SYAUP-CN-26, the cell membranes of B. cinerea mycelia were observed to be damaged under propidium iodide (PI) and transmission electron microscopy (TEM) observations. It is assumed that SYAUP-CN-26 was responsible for the damage of cell membrane. Overall, the results indicate that SYAUP-CN-26 could inhibit the growth of B. cinerea cells by damaging the cell membranes.