Exposure risk assessment of representative phthalate acid esters and associated plastic debris under the agricultural land use in typical Chinese regions.

Phthalate acid esters (PAEs) are frequently detected in the global environment and can cause potential health hazards. In this study, quantitative exposure risk assessment was undertaken to derive soil generic assessment criteria (GAC) for six representative PAEs under the agricultural land use in the evaluated Chinese regions, which coupled multi-media transport and human exposure models based on multiple exposure pathways including vegetables consumption, dermal absorption, ingestion of soil and dust, and the exposure from non-soil sources. It is identified that the PAEs in agricultural soil are dominated by DEHP and DnBP representing 72-96% of the total PAEs. The GAC for BBP and DEHP, calculated on the basis of region-specific exposure parameters and soil properties in various locations, are stringent, signifying greater potential health risks from exposure to them, warranting more rigorous contamination management. The proposed soil GAC for plastic debris are 100, 107, 73 and 88 mg kg-1 for Heilongjiang Province, Beijing City, Jiangsu and Guangdong Provinces respectively. Additionally, the potential risks of 1.68 × 10-6 and 7 × 10-6 are identified for BBP and DEHP in Guangdong Province as indicated by the exceedance of target risk level of 1 × 10-6, with the consumption of vegetables being the dominant contributor to the total estimated PAEs exposure. Overall, this methodology based on the coupled contaminant transport and exposure models incorporating region-specific data provides a technical framework to derive science-based soil GAC for representative PAEs for maintaining and assessing soil quality and food safety under the agricultural land use.

Visual tracking of label-free microplastics in wheat seedlings and their effects on crop growth and physiology.

The effects of microplastics on crop plants have attracted growing attention. However, little is known about the effects of microplastics and their extracts on the growth and physiology of wheat seedlings. In this study, hyperspectral-enhanced dark field microscopy and scanning electron microscopy were used to accurately track the accumulation of 200 nm label-free polystyrene microplastics (PS) in wheat seedlings. The PS accumulated along the root xylem cell wall and in the xylem vessel member and then moved toward to the shoots. In addition, lower concentration (≤ 5 mg·L-1) of microplastics increased root hydraulic conductivity by 80.6 %- 117.0 %. While higher PS treatment (200 mg·L-1) considerably decreased plant pigments content (chlorophyll a, b, and total chlorophyll) by 14.8 %, 19.9 %, and 17.2 %, respectively, and decreased root hydraulic conductivity by 50.7 %. Similarly, catalase activity was reduced by 17.7 % in root and 36.8 % in shoot. However, extracts from the PS solution showed no physiological effect on wheat. The result confirmed that it was the plastic particle, rather than the chemical reagents added in the microplastics, contributed to the physiological variation. These data will benefit to better understanding on the behavior of microplastics in soil plants, and to providing of convincing evidence for the effects of terrestrial microplastics.

Autophagic response of intestinal epithelial cells exposed to polystyrene nanoplastics.

Growing evidence demonstrates that the bioaccumulation of polystyrene nanoplastics (PS-NPs) in the gastrointestinal tract has negative effects on health. Until now, little information has been available regarding the potential hazards of PS-NPs to intestinal epithelial barriers. In this study, we employed cellular and animal models to investigate the adverse effects of PS-NPs on intestinal epithelium and the underlying mechanism. We found that PS-NPs affected the growth and survival of intestinal epithelial cells in a time- and concentration-dependent manner. PS-NPs accumulated in the cytoplasm, resulting in an impaired autophagic flux and inducing an autophagic response. This response was also confirmed in vivo. Our results provide new insights into the internalization of PS-NPs and the resultant autophagy response in intestinal epithelial cells.

Distribution and weathering characteristics of microplastics in paddy soils following long-term mulching: A field study in Southwest China.

Agricultural plastic-film residues have been considered as one of the important sources of microplastics in the agroecosystem. However, limited researches were conducted on the accumulation of microplastics in long-term film-mulched paddy soil. This study aims to investigate the distribution and the weathering characteristics of filmy microplastics in a mulched paddy field (non-mulch, four years of mulched, and ten years of continuous mulched soil were investigated) in Southwest China. More than 50 % of the microplastics in the mulched soil were 1-3 mm, whereas the largest percentage of the microplastics in the non-mulched soil was <1 mm (55.3 %). Microplastic compositions in this field mainly consist of polyester (PES) and polyethylene (PE) (82.1 %). The abundance of microplastics increases with the film mulching time, which were 76.2 ± 18.4, 118.6 ± 44.8, and 159.6 ± 23.5 items kg-1 in soil with non-mulching, four years of mulching, and ten years of continuous mulching, respectively. The filmy microplastics accumulated annually in the plough layer is estimated at 18.1 million items ha-1. Weathering characteristics of filmy microplastics extracted from paddy soil were characterized using FTIR, SEM-EDS, AFM, and contact angle meter. The vinyl, carbonyl, and hydroxyl indices calculated from FTIR results showed that the degradation degree of microplastics incereased as mulching time rose; compared with commercial PE films, the oxygen-containing functional groups of soil-extracted PE films were increased. This study revealed the status of microplastic pollution in paddy soil with long-term mulching. It provided primary data and a scientific basis for further study on environmental behavior and ecological impacts of microplastics in agricultural soils.

Pigment microparticles and microplastics found in human thrombi based on Raman spectral evidence.

INTRODUCTION: Environmental microparticle is becoming a global pollutant and the entire population is increasingly exposed to the microparticles from artificial materials. The accumulation of microparticles including microplastics and its subsequent effects need to be investigated timely to keep sustainable development of human society. OBJECTIVES: This study aimed to explore the accumulation of environmental particles in thrombus, the pathological structure in the blood circulation system. METHODS: Patients receiving cardiovascular surgical operations were screened and twenty-six thrombi were collected, digested and filtered. Non-soluble microparticles were enriched on the filter membrane and then were analyzed and identified with Raman Spectrometer. The associations of particle status (presence or absence) or particle number in the thrombus and clinical indicators were examined. One strict quality control-particle detection system was designed to eliminate environmental contaminations. RESULTS: Among twenty-six thrombi, sixteen contained eighty-seven identified particles ranging from 2.1 to 26.0 µm in size. The number of microparticles in each thrombus ranged from one to fifteen with the median reaching five. All the particles found in thrombi were irregularly block-shaped. Totally, twenty-one phthalocyanine particles, one Hostasol-Green particle, and one low-density polyethylene microplastic, which were from synthetic materials, were identified in thrombi. The rest microparticles included iron compounds and metallic oxides. After the adjustment for potential confounders, a significantly positive association between microparticle number and blood platelet levels was detected (P < 0.01). CONCLUSION: This study provides the first photograph and Raman spectrum evidence of microparticles in thrombi. A large number of non-soluble particles including synthetic material microparticles could accumulate in arteries, suggesting that the risk of microparticle exposure was under-estimated and the re-evaluation of its health effects is urgently needed. There will be a series of reports on assessing the health effects of microparticle exposure in humans in the future and this research provided clues for the subsequent research.

A systematic review of the impacts of exposure to micro- and nano-plastics on human tissue accumulation and health.

Micro- and nano-plastics (MNPs) pollution has become a pressing global environmental issue, with growing concerns regarding its impact on human health. However, evidence on the effects of MNPs on human health remains limited. This paper reviews the three routes of human exposure to MNPs, which include ingestion, inhalation, and dermal contact. It further discusses the potential routes of translocation of MNPs in human lungs, intestines, and skin, analyses the potential impact of MNPs on the homeostasis of human organ systems, and provides an outlook on future research priorities for MNPs in human health. There is growing evidence that MNPs are present in human tissues or fluids. Lab studies, including in vivo animal models and in vitro human-derived cell cultures, revealed that MNPs exposure could negatively affect human health. MNPs exposure could cause oxidative stress, cytotoxicity, disruption of internal barriers like the intestinal, the air-blood and the placental barrier, tissue damage, as well as immune homeostasis imbalance, endocrine disruption, and reproductive and developmental toxicity. Limitedly available epidemiological studies suggest that disorders like lung nodules, asthma, and blood thrombus might be caused or exacerbated by MNPs exposure. However, direct evidence for the effects of MNPs on human health is still scarce, and future research in this area is needed to provide quantitative support for assessing the risk of MNPs to human health.

Quantitative tracing of uptake and transport of submicrometre plastics in crop plants using lanthanide chelates as a dual-functional tracer.

The uptake pathways of nanoplastics by edible plants have recently been qualitatively investigated. There is an urgent need to accurately quantify nanoplastics accumulation in plants. Polystyrene (PS) particles with a diameter of 200 nm were doped with the europium chelate Eu-ß-diketonate (PS-Eu), which was used to quantify PS-Eu particles uptake by wheat (Triticum aestivum) and lettuce (Lactuca sativa), grown hydroponically and in sandy soil using inductively coupled plasma mass spectrometry. PS-Eu particles accumulated mainly in the roots, while transport to the shoots was limited (for example, <3% for 5,000 µg PS particles per litre exposure). Visualization of PS-Eu particles in the roots and shoots was performed with time-gated luminescence through the time-resolved fluorescence of the Eu chelate. The presence of PS-Eu particles in the plant was further confirmed by scanning electron microscopy. Doping with lanthanide chelates provides a versatile strategy for elucidating the interactions between nanoplastics and plants.

Impact of salinity on colloidal ozone aphrons in removing phenanthrene from sediments.

Polycyclic aromatic hydrocarbons (PAHs) tend to adsorb and accumulate on sediments owing to their hydrophobicity and persistence. Salinity is the predominant factor determining the PAH partition between aqueous and solid phases in freshwater, estuaries and seawater. This study focuses on the impact of salinity on the phenanthrene (PHE) removal from sediments using an in situ and targeted remediation technology - colloidal ozone aphrons (COAs). The ozone-encapsulated colloidal aphrons exhibited increasing air holdup but decreasing stability with the salinity increasing from 0.5‰ to 35‰. The hydrophobic attraction between Tween-20-coated bubbles and the hydrophobic solid surface weakened at high salinities. The presence of inorganic ions in the aqueous phase could lead to the salting-out of nonionic compounds (PHE, Tween-20 and even ozone), hindering detaching and degrading PHE from the solid phase. Anyhow, COAs achieved high efficiencies of washing (88.0-90.2%) and oxidative degradation (74.0-76.5%) particularly for the hydrophobic sediments with highly concentrated PHE (200.4 µg/kg) over the investigated salinities. The flushing effect imposed by the bubble flow played an important role, which was not greatly influenced by salinity. Although the dissolved natural organic matter competed with PHE for COAs and led to low PHE removal, the efficiency was improved by successive COA addition.

Ozone-encapsulated colloidal gas aphrons for in situ and targeting remediation of phenanthrene-contaminated sediment-aquifer.

The hydrophobic polycyclic aromatic hydrocarbons (PAHs) are apt to adhere tightly to the sediments in aquifer and thus pose great threats to the aquatic environment of groundwater and surface water as well as human health. The present study constructed functionalized microbubbles, named colloidal ozone aphrons (COAs), by dissolving ozone-contained air into the nonionic surfactant (Tween-20) solution at the pressure of 300 kPa for the in situ remediation of phenanthrene (PHE)-contaminated sediments. The COA system aimed at improving the PHE elimination in terms of (i) enhancing the migration and transportation ability of the bubble system in the contaminated aquifer matrix, (ii) accurately desorbing the target hydrophobic contaminants from sediments, and (iii) reinforcing the in situ oxidation degradation immediately after or simultaneously when the PAHs are desorbed into the aqueous phase. Experimental results demonstrated that the COAs exhibited similar characteristics as the classical colloidal gas aphrons (CGAs), including the high stability (half-life time > 200 s), typical morphology and average bubble size (114-162 µm); higher air hold-up of COAs was achieved (i. e. > 20%) compared with the air-microbubbles (1-2%) obtained under the same generation conditions. Although the encapsulated ozone could oxidize the surfactant-layers, the properties and behaviors of COAs were not greatly affected. The surfactant multi-layers endowed the COAs with strong hydrophobic attraction with PHE, great migration capacity and enlarged oxidation area in the sediment matrix. Approximately 96.9% of PHE was removed from the sediments and 84.9% of the overall PHE was oxidized at the high ozone concentration of 0.6 mg/L when the initial PHE concentration was 240.0 µg/kg. The COA-involved remediation technology provided the insight of combining the processes of washing and oxidizing through adopting the particularly conceived microbubbles. The in situ and selective removal of hydrophobic organic contaminants from sediments in aquifer was well achieved in this study.

Microbiological and environmental significance of metal-dependent anaerobic oxidation of methane.

Anaerobic oxidation of methane (AOM) can be coupled to the reduction of sulfate, nitrate and nitrite, which effectively reduces methane emission into the atmosphere. Recently, metal-dependent AOM (metal-AOM, AOM coupled to metal reduction) was demonstrated to occur in both environmental samples and enrichment cultures. Anaerobic methanotrophs are capable of respiration using Fe(III) or Mn(IV), whether they are in the form of soluble metal species or insoluble minerals. Given the wide distribution of Fe(III)/Mn(IV)-bearing minerals in aquatic methane-rich environments, metal-AOM is considered to be globally important, although it has generally been overlooked in previous studies. In this article, we discuss the discovery of this process, the microorganisms and mechanisms involved, environmental significance and factors influencing metal-AOM. Since metal-AOM is poorly studied to date, some discussion is included on the present understanding of sulfate- and nitrate-AOM and traditional metal reduction processes using organic substrates or hydrogen as electron donors. Metal-AOM is a relatively new research field, and therefore more studies are needed to fully characterize the process. This review summarizes current studies and discusses the many unanswered questions, which should be useful for future research in this field.

Oxygenic denitrification for nitrogen removal with less greenhouse gas emissions: Microbiology and potential applications.

Nitrogen pollution is a worldwide problem and has been extensively treated by canonical denitrification (CDN) process. However, the CDN process generates several issues such as intensive greenhouse gas (GHG) emissions. In the past years, a novel biological nitrogen removal (BNR) process of oxygenic denitrification (O2DN) has been proposed as a promising alternative to the CDN process. The classic denitrification four steps are simplified to three steps by O2DN bacteria without producing and releasing the intermediate nitrous oxide (N2O), a potent GHG. In this article, we summarized the findings in previous literatures as well as our results, including involved microorganisms and metabolic mechanisms, functional genes and microbial detection, kinetics and influencing factors and their potential applications in wastewater treatment. Based on our knowledge and experience, the benefits and limitations of the current O2DN process were analyzed. Since O2DN is a new field in wastewater treatment, more research and application is required, especially the development of integrated processes and the quantitative assessment of the contribution of O2DN process in natural habitats and engineered systems.

A comparison of conditional random fields and structured support vector machines for chemical entity recognition in biomedical literature.

BACKGROUND: Chemical compounds and drugs (together called chemical entities) embedded in scientific articles are crucial for many information extraction tasks in the biomedical domain. However, only a very limited number of chemical entity recognition systems are publically available, probably due to the lack of large manually annotated corpora. To accelerate the development of chemical entity recognition systems, the Spanish National Cancer Research Center (CNIO) and The University of Navarra organized a challenge on Chemical and Drug Named Entity Recognition (CHEMDNER). The CHEMDNER challenge contains two individual subtasks: 1) Chemical Entity Mention recognition (CEM); and 2) Chemical Document Indexing (CDI). Our study proposes machine learning-based systems for the CEM task. METHODS: The 2013 CHEMDNER challenge organizers provided a manually annotated 10,000 UTF8-encoded PubMed abstracts according to a predefined annotation guideline: a training set of 3,500 abstracts, a development set of 3,500 abstracts and a test set of 3,000 abstracts. We developed machine learning-based systems, based on conditional random fields (CRF) and structured support vector machines (SSVM) respectively, for the CEM task for this data set. The effects of three types of word representation (WR) features, generated by Brown clustering, random indexing and skip-gram, on both two machine learning-based systems were also investigated. The performance of our system was evaluated on the test set using scripts provided by the CHEMDNER challenge organizers. Primary evaluation measures were micro Precision, Recall, and F-measure. RESULTS: Our best system was among the top ranked systems with an official micro F-measure of 85.05%. Fixing a bug caused by inconsistent features marginally improved the performance (micro F-measure of 85.20%) of the system. CONCLUSIONS: The SSVM-based CEM systems outperformed the CRF-based CEM systems when using the same features. Each type of the WR feature was beneficial to the CEM task. Both the CRF-based and SSVM-based systems using the all three types of WR features showed better performance than the systems using only one type of the WR feature.

Serum cystatin C is an early biomarker for assessment of renal function in burn patients.

BACKGROUND: Early detection of renal dysfunction is important in burn patients. This study evaluated whether serum cystatin C (CysC) is a potentially accurate and sensitive marker for identification of reduced glomerular filtration rate (GFR) and the risk factors of impaired renal function in major burn patients. METHODS: A total of 48 adult patients with major burn injury were enrolled. Renal function was assessed using serum creatinine (sCr), 24-h urinary creatinine clearance (24-hCrCl), sCr-based formulae and CysC-based formulae on the second day and seventh day post-burn. RESULTS: There was a high prevalence (27.1%) of acute impaired renal function in major burn patients in the first week post-burn. CysC-based formulae for estimated GFR (eGFR) are more accurate and sensitive for detection of impaired renal function than sCr-based formulae. Multivariate logistic regression analysis demonstrated that age (OR, 2.08; 95% CI 1.26-4.77) and the percentage of burn area (OR, 3.41; 95% CI 1.64-6.95) were independent risk factors of impaired renal function. CONCLUSIONS: The results of this study suggest that CysC is a more accurate and sensitive marker for identification of acute impaired renal function in major burn patients than sCr. It is important to measure CysC and calculate eGFR to prevent acute renal failure and modify drug doses in burn patients, especially those of older age and with major burn areas.