A spatially continuous diffusion model of visual working memory.

We present results from five visual working memory (VWM) experiments in which participants were briefly shown between 2 and 6 colored squares. They were then cued to recall the color of one of the squares and they responded by choosing the color on a continuous color wheel. The experiments provided response proportions and response time (RT) measures as a function of angle for the choices. Current VWM models for this task include discrete models that assume an item is either within working memory or not and resource models that assume that memory strength varies as a function of the number of items. Because these models do not include processes that allow them to account for RT data, we implemented them within the spatially continuous diffusion model (SCDM, Ratcliff, 2018) and use the experimental data to evaluate these combined models. In the SCDM, evidence retrieved from memory is represented as a spatially continuous normal distribution and this drives the decision process until a criterion (represented as a 1-D line) is reached, which produces a decision. Noise in the accumulation process is represented by continuous Gaussian process noise over spatial position. The models that fit best from the discrete and resource-based classes converged on a common model that had a guessing component and that allowed the height of the normal memory-strength distribution to vary with number of items. The guessing component was implemented as a regular decision process driven by a flat evidence distribution, a zero-drift process. The combination of choice and RT data allows models that were not identifiable based on choice data alone to be discriminated.

Does response modality influence conflict? Modelling vocal and manual response Stroop interference.

In the Stroop task, color words are presented in colored fonts and the task of the subject is to either name the word or name the color. If the word and font color are in agreement, then the stimulus is said to be congruent (e.g., RED in red font color); however, if the word and font color are not in agreement, the stimulus is said to be incongruent (e.g., RED in blue font color). Conflict in the Stroop task is measured by both RT and accuracy. In prior research, the amount of conflict differs depending on the response modality, vocal versus manual. We applied a model for multichoice decision-making (and confidence), the RTCON2 model (Ratcliff & Starns, 2013) to the data from 4 experiments, 2 with 2-choice manual responses, 1 with 4-choice manual touch screen responses, 1 with both 4-choice vocal responses, and 4-choice manual keyboard responses. Changes in the rate of information accumulation captured conflict effects for the manual-response versions, but not for the vocal-response version. Adding an extra nondecision time parameter allowed RTCON2 to account for the data patterns in the vocal-response version. However, to fully understand conflict in the vocal-response Stroop task, a model of conflict processing in the vocal word production system must be developed that would explain the additional processing time in the nondecision time parameter. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

The telomere bouquet regulates meiotic centromere assembly.

The role of the conserved meiotic telomere bouquet has been enigmatic for over a century. We showed previously that disruption of the fission yeast bouquet impairs spindle formation in approximately half of meiotic cells. Surprisingly, bouquet-deficient meiocytes with functional spindles harbour chromosomes that fail to achieve spindle attachment. Kinetochore proteins and the centromeric histone H3 variant Cnp1 fail to localize to those centromeres that exhibit spindle attachment defects in the bouquet's absence. The HP1 orthologue Swi6 also fails to bind these centromeres, suggesting that compromised pericentromeric heterochromatin underlies the kinetochore defects. We find that centromeres are prone to disassembly during meiosis, but this is reversed by localization of centromeres to the telomere-proximal microenvironment, which is conducive to heterochromatin formation and centromere reassembly. Accordingly, artificially tethering a centromere to a telomere rescues the tethered centromere but not other centromeres. These results reveal an unanticipated level of control of centromeres by telomeres.

Telomeres and centromeres have interchangeable roles in promoting meiotic spindle formation.

Telomeres and centromeres have traditionally been considered to perform distinct roles. During meiotic prophase, in a conserved chromosomal configuration called the bouquet, telomeres gather to the nuclear membrane (NM), often near centrosomes. We found previously that upon disruption of the fission yeast bouquet, centrosomes failed to insert into the NM at meiosis I and nucleate bipolar spindles. Hence, the trans-NM association of telomeres with centrosomes during prophase is crucial for efficient spindle formation. Nonetheless, in approximately half of bouquet-deficient meiocytes, spindles form properly. Here, we show that bouquet-deficient cells can successfully undergo meiosis using centromere-centrosome contact instead of telomere-centrosome contact to generate spindle formation. Accordingly, forced association between centromeres and centrosomes fully rescued the spindle defects incurred by bouquet disruption. Telomeres and centromeres both stimulate focal accumulation of the SUN domain protein Sad1 beneath the centrosome, suggesting a molecular underpinning for their shared spindle-generating ability. Our observations demonstrate an unanticipated level of interchangeability between the two most prominent chromosomal landmarks.

A single internal telomere tract ensures meiotic spindle formation.

Contact between telomeres and the fission yeast spindle pole body during meiotic prophase is crucial for subsequent spindle assembly, but the feature of telomeres that confers their ability to promote spindle formation remains mysterious. Here we show that while strains harbouring circular chromosomes devoid of telomere repeat tracts undergo aberrant meiosis with defective spindles, the insertion of a single internal telomere repeat stretch rescues the spindle defects. Moreover, the telomeric overhang-binding protein Pot1 is dispensable for rescue of spindle formation. Hence, an inherent feature of the double-strand telomeric region endows telomeres with the capacity to promote spindle formation.