RESUMO
Acute respiratory infections (ARIs) constitute one of the leading causes of antibiotic administration, hospitalization and death among children <5 years old. The upper respiratory tract microbiota has been suggested to explain differential susceptibility to ARIs and modulate ARI severity. The aim of the present study was to investigate the relation of nasopharyngeal microbiota and other microbiological parameters with respiratory health and disease, and to assess nasopharyngeal microbiota diagnostic utility for discriminating between different respiratory health statuses. We conducted a prospective case-control study at Hospital Sant Joan de Deu (Barcelona, Spain) from 2014 to 2018. This study included three groups of children <18 years with gradual decrease of ARI severity: cases with invasive pneumococcal disease (IPD) (representative of lower respiratory tract infections and systemic infections), symptomatic controls with mild viral upper respiratory tract infections (URTI), and healthy/asymptomatic controls according to an approximate case-control ratio 1:2. Nasopharyngeal samples were collected from participants for detection, quantification and serotyping of pneumococcal DNA, viral DNA/RNA detection and 16S rRNA gene sequencing. Microbiological parameters were included on case-control classification models. A total of 140 subjects were recruited (IPD=27, URTI=48, healthy/asymptomatic control=65). Children's nasopharyngeal microbiota composition varied according to respiratory health status and infection severity. The IPD group was characterized by overrepresentation of Streptococcus pneumoniae, higher frequency of invasive pneumococcal serotypes, increased rate of viral infection and underrepresentation of potential protective bacterial species such as Dolosigranulum pigrum and Moraxella lincolnii. Microbiota-based classification models differentiated cases from controls with moderately high accuracy. These results demonstrate the close relationship existing between a child's nasopharyngeal microbiota and respiratory health, and provide initial evidence of the potential of microbiota-based diagnostics for differential diagnosis of severe ARIs using non-invasive samples.
Assuntos
Nível de Saúde , Microbiota , Nasofaringe/microbiologia , Sistema Respiratório/microbiologia , Adolescente , Bactérias/genética , Carnobacteriaceae , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Microbiota/genética , Moraxella , Infecções Pneumocócicas/microbiologia , Estudos Prospectivos , RNA Ribossômico 16S/genética , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/microbiologia , Streptococcus pneumoniae/genéticaRESUMO
Se efectúa un recorrido histórico hasta llegar al momento en que comienza a utilizarse la propia sangre para transfundir. Se analizan los diferentes tipos de transfusión autóloga, para pasar a detallar específicamente el sistema de transfusión autóloga rápida de Baylor. Se describen sus aplicaciones clínicas y las ventajas que presenta en cuanto a recuperación intraoperatoria, en disminución de riesgos de transmisión de infecciones y la mejor aceptación del paciente (AU)
