RESUMO
Two new indolocarbazole alkaloids, 4'-N-methyl-5'-hydroxystaurosporine (2) and 5'-hydroxystaurosporine (3), were isolated together with the known staurosporine (1) from the culture broth of a marine Micromonospora sp. (strain L-31-CLCO-002). The fermentation, structural data and cytotoxic activities of these compounds against various tumor cell lines are given.
Assuntos
Antineoplásicos/isolamento & purificação , Carbazóis/isolamento & purificação , Indóis/isolamento & purificação , Micromonospora/química , Alcaloides/química , Alcaloides/isolamento & purificação , Alcaloides/farmacologia , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Carbazóis/química , Carbazóis/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Células HT29 , Humanos , Indóis/química , Indóis/farmacologia , Concentração Inibidora 50 , Espectroscopia de Ressonância Magnética , Camundongos , Micromonospora/crescimento & desenvolvimento , Micromonospora/metabolismo , Estaurosporina/análogos & derivados , Células Tumorais Cultivadas , Microbiologia da ÁguaRESUMO
A novel bioactive macrolide, IB-96212 has been isolated from the fermentation broth of a marine actinomycete, L-25-ES25-008. The strain belongs to the genus Micromonospora. The macrolide showed a very strong cytotoxic activity against P-388, and lower but significant activity against A-549, HT-29, and MEL-28 cell lines. We describe the isolation, taxonomy and fermentation of the producing strain as well as the isolation of IB-96212.
