RESUMO
The present study aimed to examine the phenolic content and evaluate the antimicrobial and antioxidant potential of ethanol extracts from the moss species Phyllogonium viride Brid. on the pathogenic bacteria Salmonella enterica serovar enteritidis, Staphylococcus aureus, Listeria monocytogenes and Escherichia coli, and the pathogenic fungi Candida albicans and Cryptococcus neoformans. The antimicrobial activity was determined from Minimum Inhibitory Concentration (MIC) Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC). Antioxidant activity was determined by the DPPH method. Folin-Denis reagent was used for the content of total phenolics and flavonoids and HPLC-DAD for identification of phenolic compounds. The results showed that bacteriostatic and bactericidal activities occurred at concentrations ranging from 9.76â µg/mL-78.13â µg/mL among all evaluated microorganisms. These values, considering the criteria used, suggest the P. viride extract as a potent antimicrobial. For antioxidant activity, P. viride extract was considered weak. Analysis of the phenolic content showed a wide range of compounds, with Kaempferol (0.41â mg/g) being the major compound, followed by t-cinnamic acid and caffeic acid (0.17â mg/g). Although P. viride is a species of moss not yet referenced in scientific publications of biotechnological interest, it has shown promising potential for further studies and possible application as an antimicrobial of natural origin.
Assuntos
Briófitas , Etanol , Antioxidantes/farmacologia , Candida albicans , Cromatografia Líquida de Alta Pressão , Escherichia coli , Extratos Vegetais/farmacologiaRESUMO
Opuntia monacantha is a Cactaceae found in regions with few nutrients and water. This study aimed to identify bioactive compounds in the fruit of O. monacantha in the southern region of Brazil. In the fruit of O. monacantha, five phenolic compounds were identified: protocatechuic acid, ferulic acid, p-coumaric acid, catechin and quercetin. The ferulic acid and catechin were found in higher concentration in the fruit. The terpenes α-amyrin, ß-amyrin, oleanolic acid and cycloartenol were also identified in the hexane fractions of the fruit. Unsaturated fatty acids were found in the fruit seed, with the highest concentration of linoleic acid (69.0%).
Assuntos
Opuntia , Opuntia/química , Frutas/química , Fenóis/química , Fenóis/farmacologiaRESUMO
The orange-fleshed sweet potato is a vegetable-rich in carotenoids. The thermic treatment for sweet potato processing can decrease the content of these constituents in the foods, lowering their bioactive properties. Raman spectroscopy has been growing as a fast tool to food analysis, especially for detection of low concentrations of carotenoids and to the monitoring of its degradation profile over time. Therefore, in this work were evaluated two methods of drying, hot air and microwaving with rotary drum, combined with quantitative Raman spectroscopy. The results showed carotenoids degradation around 50% for both types of drying processes studied. PCA plot proved the potential of reproducibility of analyses for microwave drying samples. For samples heated with hot air, the best linear correlation achieved was R2â¯=â¯0.90 and by microwave was R2â¯=â¯0.88. Also, partial least squares (PLS) regression models were constructed obtaining a satisfactory coefficient of determination.