RESUMO
Cultures of Agaricus bisporus, the most extensively cultivated mushroom, can be infected severely by Pseudomonas tolaasii. This pathogen is characterized by the so-called white line reaction, a precipitate formed on agar plates between its colonies and those of P. reactans, both belonging to the collective species P. fluorescens. A recent study has shown that a group of P. tolaasii isolates can be subdivided into two groups or 'siderovars', based on the pyoverdines they produce (Munsch et al. 2000). One group of strains is characterized by the pyoverdine described by Demange et al. (1990). A representative of the second group (strain Ps3a) was found to produce the same pyoverdine as a strain which had been classified before as P. aureofaciens. However, based mainly on 16S rRNA gene sequence comparisons and REP-PCR generated fingerprints, the two strains are not identical. They are also distinguishable from the P. tolaasii type strain.
Assuntos
Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/química , Oligopeptídeos , Pigmentos Biológicos/biossíntese , Pigmentos Biológicos/química , Pseudomonas/metabolismo , Sideróforos/biossíntese , Sideróforos/química , Agaricales , Sequência de Aminoácidos , Microbiologia de Alimentos , Estrutura Molecular , Pseudomonas/genética , Pseudomonas/isolamento & purificação , Pseudomonas/patogenicidade , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Especificidade da EspécieRESUMO
From a Pseudomonas aeruginosa hospital isolate a new pyoverdin was isolated. It is identical with that of Pseudomonas aeruginosa strain R except that in the peptide chain L-Gln is missing.
