Mulinum crassifolium Phil; Two New Mulinanes, Gastroprotective Activity and Metabolomic Analysis by UHPLC-Orbitrap Mass Spectrometry.

Mulinum crassifolium Phil. (Apiaceae) is an endemic shrub from Chile commonly used as infusion in traditional medicine to treat diabetes, bronchial and intestinal disorders and stomach ailments, including ulcers. From the EtOAc extract of this plant, the new mulinane-type diterpenoids 3 and 5 were isolated along with three known diterpenoids. The gastroprotective effect of the infusion of the plant was assayed to support the traditional use and a fast HPLC analysis using high resolution techniques was performed to identify the bioactive constituents. The EtOAc extract and the edible infusion showed gastroprotective effect at 100 mg/kg in the HCl/EtOH induced gastric ulcer model in mice, reducing lesions by 33% and 74%, respectively. Finally, a metabolomic profiling based on UHPLC-ESI-MS/HRMS of the edible infusion was performed and thirty-five compounds were tentatively identified including quercetin, caffeic acid, apigenine glucoside, p-coumaric acid, chlorogenic acids, and caffeoylquinic acids, which have been associated previously with gastroprotective and antiulcer properties. This scientific evidence can support the contribution of polyphenols in the gastroprotective activity of the edible infusion of this plant, and can validate at least in part, its ethnopharmacological use.

Biological activity and chemical characterization of Pouteria lucuma seeds: A possible use of an agricultural waste.

Pouteria lucuma fruit is widely used to prepare cakes, ice creams and juice or also commercialized as pulp and flour. As result of this business thousands of tons of seeds are generated as an agricultural waste. This study presents the antioxidant and antiulcer activities, and the identification of secondary metabolites by UHPLC/ESI/MS/MS of an agroindustrial waste of Pouteria lucuma seeds. Fifty-nine compounds were tentatively identified including eight aminoacids, five organic acids, one nucleoside, five phenolic acids, five phenolic alcohols, nineteen flavonoids, six lipids, and seven unknowns in the methanol extract of P. lucuma seeds. The total phenolic content of the seeds was 52.82 ±â€¯0.09 µmol GAE/g dry weight, while total flavonoid content was 5.99 ±â€¯0.01 µmol Q/g dry weight. The antioxidant activity was 58.14 ±â€¯0.05, 66.97 ±â€¯0.00, 272.50 ±â€¯0.00, and 67.02 ±â€¯2.23 for the DPPH, ABTS, FRAP, and superoxide anion assays, respectively. The highest gastroprotective activity was obtained at 100 mg/kg (78%), which as higher than the positive control lansoprazole (75%). Our findings showed that P. lucuma seed extracts have moderate to high antioxidant activity and gastroprotective properties. Therefore, it was demostrated that lucuma seeds commonly eliminated as an agricultural industry waste, could be useful for the preparation of nutritional supplements.