Effects of aqueous extracts of Caryocar brasiliense in mice / Efeitos de extratos aquosos de Caryocar brasiliense em camundongos

Background: Caryocar brasiliense Cambess (Caryocaraceae) is an arboreal plant native of Brasilian Cerrado and its fruit is an important source of food and income for the human population of this region. In addition to using as food, different parts of this tree have great medicinal potential as antimicrobial, anti-inflammatory, antioxidant and antihelmintic. However, the active metabolites those are likely to confer these pharmacological properties healing may also be toxic dependent upon the dose and route of administration. In this quest, the aim of this study was to assess the acute toxicity of aqueous extracts of fruit peels and leaves of C. brasiliense in mice of both genders. Materials, Methods & Results: The aqueous extracts were obtained by decoction, diluted in water and filtered through membrane. The toxicity of extract of fruit peels (62.5 at 500 mg/kg of body weight (BW) and leaf extract (18.75 mg/kg bw) were evaluated in males and females mice (Mus musculus) Swiss by intraperitoneal route. For 2 control groups were administered injection water. The clinical signs and deaths were recorded up to 14 days after administration. The lethal doses for 10 (LD10) or 50 (LD50) % of population were estimated with Probit regression analysis. The Chi-square test was used to analyze differences of mortality frequencies between males and females. The groups treated with the [...]

Effects of aqueous extracts of Caryocar brasiliense in mice / Efeitos de extratos aquosos de Caryocar brasiliense em camundongos

Background: Caryocar brasiliense Cambess (Caryocaraceae) is an arboreal plant native of Brasilian Cerrado and its fruit is an important source of food and income for the human population of this region. In addition to using as food, different parts of this tree have great medicinal potential as antimicrobial, anti-inflammatory, antioxidant and antihelmintic. However, the active metabolites those are likely to confer these pharmacological properties healing may also be toxic dependent upon the dose and route of administration. In this quest, the aim of this study was to assess the acute toxicity of aqueous extracts of fruit peels and leaves of C. brasiliense in mice of both genders. Materials, Methods & Results: The aqueous extracts were obtained by decoction, diluted in water and filtered through membrane. The toxicity of extract of fruit peels (62.5 at 500 mg/kg of body weight (BW) and leaf extract (18.75 mg/kg bw) were evaluated in males and females mice (Mus musculus) Swiss by intraperitoneal route. For 2 control groups were administered injection water. The clinical signs and deaths were recorded up to 14 days after administration. The lethal doses for 10 (LD10) or 50 (LD50) % of population were estimated with Probit regression analysis. The Chi-square test was used to analyze differences of mortality frequencies between males and females. The groups treated with the [...](AU)

In vitro efficacy of aqueous plants extracts in the control of gastrointestinal nematodes of cattle / Eficácia in vitro de extratos aquosos de plantas no controle de nematódeos gastrintestinais de bovinos

Background: Gastrointestinal helminthes in cattle had prejudge performance and productivity of animals. Affect beef cattleand dairy, generating huge economic losses. Commercial anthelmintics have been administered improperly, promotingthe selection of resistant populations, in addition to increasing production costs and waste accumulate in animal productsand the environment. It is therefore of great importance to find new alternatives as control. In this quest, was aimed withthis study to evaluate the in vitro efficacy of plant aqueous extracts in the inhibition of larval development assay (ILDA)and inhibition of hatchability assay (IHA) of gastrointestinal nematodes of cattle.Materials, Methods & Results: The ILDA was performed by quantitative coproculture , with aqueous extracts of the leavesof Acrocomia aculeata, Anacardium occidentale, Caryocar brasiliense, Cymbopogon citratus, Mangifera indica var. Ubáand Musa spp. cv. Prata-anã on the concentration of 100 mg/mL. In the IHA was evaluated the aqueous extracts of leavesand fruit peels of C. brasiliense, at the concentrations 15.00, 7.50, 3.75 and 1.88 mg/mL. The controls of assays wereivermectin (16 μg/mL) and levamisole phosphate solution (15 mg/ml), respectively, and water in both tests. Efficiencieswere calculated and count data submitted to analysis of variance and the means were compared by Tukey test (P < 0.01).With the probit regression analysis, we determined the lethal concentration for inhibition of 50 and 90% hatchability (LC50and LC90). Still, the levels of condensed tannins from aqueous extracts of the leaves and fruit peels of C. brasiliense werequantified. In ILDA, the extract of C. brasiliense showed efficacy of 91.29% and did not differ from ivermectin (P < 0.01).The other extracts did not obtain satisfactory efficacy and were similar to water (P < 0.01). In IHA the efficacy was over...

In vitro efficacy of aqueous plants extracts in the control of gastrointestinal nematodes of cattle / Eficácia in vitro de extratos aquosos de plantas no controle de nematódeos gastrintestinais de bovinos

Background: Gastrointestinal helminthes in cattle had prejudge performance and productivity of animals. Affect beef cattleand dairy, generating huge economic losses. Commercial anthelmintics have been administered improperly, promotingthe selection of resistant populations, in addition to increasing production costs and waste accumulate in animal productsand the environment. It is therefore of great importance to find new alternatives as control. In this quest, was aimed withthis study to evaluate the in vitro efficacy of plant aqueous extracts in the inhibition of larval development assay (ILDA)and inhibition of hatchability assay (IHA) of gastrointestinal nematodes of cattle.Materials, Methods & Results: The ILDA was performed by quantitative coproculture , with aqueous extracts of the leavesof Acrocomia aculeata, Anacardium occidentale, Caryocar brasiliense, Cymbopogon citratus, Mangifera indica var. Ubáand Musa spp. cv. Prata-anã on the concentration of 100 mg/mL. In the IHA was evaluated the aqueous extracts of leavesand fruit peels of C. brasiliense, at the concentrations 15.00, 7.50, 3.75 and 1.88 mg/mL. The controls of assays wereivermectin (16 μg/mL) and levamisole phosphate solution (15 mg/ml), respectively, and water in both tests. Efficiencieswere calculated and count data submitted to analysis of variance and the means were compared by Tukey test (P < 0.01).With the probit regression analysis, we determined the lethal concentration for inhibition of 50 and 90% hatchability (LC50and LC90). Still, the levels of condensed tannins from aqueous extracts of the leaves and fruit peels of C. brasiliense werequantified. In ILDA, the extract of C. brasiliense showed efficacy of 91.29% and did not differ from ivermectin (P < 0.01).The other extracts did not obtain satisfactory efficacy and were similar to water (P < 0.01). In IHA the efficacy was over...(AU)