RESUMO
The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.
Assuntos
Coleta de Amostras Sanguíneas/métodos , Lipídeos/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise Química do Sangue , Preservação de Sangue , Coleta de Amostras Sanguíneas/instrumentação , Coleta de Amostras Sanguíneas/normas , Colesterol/sangue , Feminino , Humanos , Laboratórios/normas , Lipoproteínas/sangue , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Temperatura , Fatores de Tempo , Triglicerídeos/sangue , Adulto JovemAssuntos
Aterosclerose/tratamento farmacológico , Azetidinas/administração & dosagem , Biomarcadores/sangue , Endotélio Vascular/metabolismo , Inibidores de Hidroximetilglutaril-CoA Redutases/administração & dosagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticolesterolemiantes/administração & dosagem , Aterosclerose/sangue , Relação Dose-Resposta a Droga , Quimioterapia Combinada , Ezetimiba , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados Aleatórios como AssuntoRESUMO
We reported a case of septicemia by Vibrio cholerae O1, in São Paulo, Brazil. A 70-year-old male patient, living in an urban area, entered the emergency service having sepsis, dying 12 hours later. Blood culture was positive for Vibrio cholerae O1. This is the first case of bacteremia by Vibrio cholerae O1 reported in South America.
Assuntos
Bacteriemia/microbiologia , Vibrioses/microbiologia , Vibrio cholerae O1/isolamento & purificação , Idoso , Humanos , Masculino , Reação em Cadeia da Polimerase , Vibrioses/diagnóstico , Vibrio cholerae O1/genéticaRESUMO
We reported a case of septicemia by Vibrio cholerae O1, in São Paulo, Brazil. A 70-year-old male patient, living in an urban area, entered the emergency service having sepsis, dying 12 hours later. Blood culture was positive for Vibrio cholerae O1. This is the first case of bacteremia by Vibrio cholerae O1 reported in South America.
