Secondary analysis of one State's Youth Risk Behavior Surveillance System (YRBSS) data by Individualized Education Program (IEP) status.

BACKGROUND: Children with disabilities may be at increased risk for engaging in health risk behaviors compared to their peers without disabilities. OBJECTIVE: This secondary analysis aims to assess if Individualized Education Program (IEP) status, a proxy for having a disability, is a risk factor for youth to engage in health risk behaviors such as alcohol use, marijuana use, other substance use, bullying or cyberbullying victimization, and sexual activity. METHODS: Data from Connecticut Youth Risk Behavior Surveillance System (YRBSS) 2013, 2015, 2017, and 2019 were combined for a representative sample of 9243 students, 850 reporting having an IEP. Having a disability was measured by an item that asked if participants received special education services as part of an IEP. Logistic regression that accounted for the YRBSS sample design was used to assess main effects. RESULTS: Having an IEP significantly predicted the likelihood of being bullied (OR = 1.81), cyberbullied (OR = 1.49), and other drug use (OR = 1.65), but did not predict engaging in sexual activity. CONCLUSIONS: Students with disabilities in CT, as defined by the receipt of special education services as specified on an IEP, are at increased risk to engage in health risk behaviors than are students without disabilities during their high school years. Further analyses and comparisons across sites, years, and type of disability are limited as there is no current item on the national YRBSS questionnaire that measures a participant's special education status or disability status.

Assessment of TSPAN Expression Profile and Their Role in the VSCC Prognosis.

The role and prognostic value of tetraspanins (TSPANs) in vulvar squamous cell carcinoma (VSCC) remain poorly understood. We sought to primarily determine, at both the molecular and tissue level, the expression profile of the TSPANs CD9, CD63, CD81, and CD82 in archived VSCC samples (n = 117) and further investigate their clinical relevance as prognostic markers. Our studies led us to identify CD63 as the most highly expressed TSPAN, at the gene and protein levels. Multicomparison studies also revealed that the expression of CD9 was associated with tumor size, whereas CD63 upregulation was associated with histological diagnosis and vascular invasion. Moreover, low expression of CD81 and CD82 was associated with worse prognosis. To determine the role of TSPANs in VSCC at the cellular level, we assessed the mRNA levels of CD63 and CD82 in established metastatic (SW962) and non-metastatic (SW954) VSCC human cell lines. CD82 was found to be downregulated in SW962 cells, thus supporting its metastasis suppressor role. However, CD63 was significantly upregulated in both cell lines. Silencing of CD63 by siRNA led to a significant decrease in proliferation of both SW954 and SW962. Furthermore, in SW962 particularly, CD63-siRNA also remarkably inhibited cell migration. Altogether, our data suggest that the differential expression of TSPANs represents an important feature for prognosis of VSCC patients and indicates that CD63 and CD82 are likely potential therapeutic targets in VSCC.

O uso de plantas medicinais para o tratamento do Diabetes Mellitus Tipo II ­ Revisão de literatura / The use of medicinal plants for treatment of type II Diabetes Mellitus ­ Literature review

O Diabetes mellitus tipo II (DM II) é uma síndrome metabólica com uma das maiores prevalências mundiais. A Organização Mundial da Saúde (OMS, 2019) estima que até 2045 teremos aproximadamente 629 milhões de diabéticos adultos no planeta. Além disso, a Federação Internacional de Diabetes (IDF, 2019) estima que o gasto anual global com tratamentos para diabetes adultos em 2017 foi de U$850 bilhões de dólares. Com números tão expressivos buscam-se tratamentos naturais efetivo, eficaz e baixo custo para tal distúrbio. Inúmeras plantas já estão sendo utilizadas como coadjuvante no tratamento do DM II por possuírem ação antidiabética. Porém, há uma incidência na procura de ampliar o acervo de plantas medicinais com compostos hipoglicemiantes. Portanto, este presente trabalho destaca plantas como Croton cajucara Benth, Licania rígida Benth e Morus nigra L. e seus compostos hipoglicemiantes como alternativa em potencial para tratamento desta síndrome metabólica. Os resultados demonstraram a importância das mesmas para a terapêutica do DM II com estudos que comprovem a ação, garantia da eficácia e segurança em sua utilização.

Diabetes mellitus type II (DM II) is a metabolic syndrome with one of the highest prevalence worldwide. The World Health Organization (WHO, 2019) estimates that by 2045 we will have approximately 629 milion adult diabetics on the planet. In addition, the International Diabetes Federation (IDF, 2019) estimates that the annual global expenditure on treatments for adult diabetes in 2017 was $ 850 bilion dollars. With such expressive numbers, effective, efficient and lowcost natural treatments are sought for such disorder. Numerous plants are already being used as an adjuvant in the treatment of DM II because they have an anti-diabetc action. However, there is an incidence in the search to expand the collection of medicinal plants with hypoglicemic compounds. Therefore, this present work highligths plants such as Croton cajucara Benth, Licania rigida Benth and Morus nigra L., and their hypoglycemic compounds as a potential alternative for the treatment of this metabolic syndrome. The results demonstrated the importance of the same for the treatment of DM II with studies that prove the action, guarantee of the efficacy and safety in its use.

Suicide Postvention for the United States Military: Literature Review, Conceptual Model, and Recommendations.

Suicide continues to be a significant public health problem in the United States and the Department of Defense (DoD). Timely and systematic postvention efforts can play an instrumental role in helping family members, peers, and military command to best manage the aftermath of a suicide. To date, several postvention efforts have been implemented in the military. However, there continues to be an overall lack of understanding of the specific short- and long-term effects of exposure to military suicide. In addition, more emphasis needs to be placed on empirically driven approaches to postvention and program evaluation. The purpose of this article is threefold: (1) to provide a summary of the postvention literature with special emphasis placed on the military organization; (2) to propose a conceptual model as a framework for understanding Military-Unit Suicide Survivorship; and (3) to briefly highlight postvention strategies within the DoD in the context of a number of research, clinical, and policy recommendations.

Effects of tannery wastewater exposure on adult Drosophila melanogaster.

Our aim was to evaluate the effects of exposure to tannery wastewater on mortality and/or antioxidant enzyme system in adult wild-type Canton-S Drosophila melanogaster. Exposure to tannery wastewater induced a concentration-dependent lethality in adult Canton-S flies. Tannery wastewater was able to alter antioxidant enzyme activities, specifically glutathione peroxidase-like and glutathione S-transferase, in adult Canton-S D. melanogaster. We conclude that D. melanogaster is a reliable model to evaluate the toxicity induced by tannery wastewater.

Experimental Post-traumatic Stress Disorder Decreases Astrocyte Density and Changes Astrocytic Polarity in the CA1 Hippocampus of Male Rats.

Post-traumatic stress disorder (PTSD) is a psychiatric condition resulting from exposure to a traumatic event. It is characterized by several debilitating symptoms including re-experiencing the past trauma, avoidance behavior, increased fear, and hyperarousal. Key roles in the neuropathology of PTSD and its symptomatology have been attributed to the hippocampus and amygdala. These regions are involved in explicit memory processes and context encoding during fear conditioning. The aim of our study was to investigate whether PTSD is capable of altering the morphology, density and expression of glial fibrillary acidic protein (GFAP) in astrocytes from the CA1 region of the hippocampus and the medial amygdala and correlate the data obtained with the orientation index of the polarity of astrocytes. Thirty male rats were divided in two groups: control (n = 15) and PTSD (n = 15). The inescapable shock protocol, in which the animals are exposed to a single episode of footshock, was used to induce PTSD. Our results show that, in the hippocampus, PTSD is capable of decreasing the density of GFAP+ astrocytes as well as altering astrocytic morphology, as shown by the reductions observed in the total number of primary processes, in the number of primary processes in the lateral quadrants, and the degree of branching in the lateral quadrants. The analysis of the orientation index indicates that PTSD alters the polarity of hippocampal astrocytes. No alterations were observed in the amygdala astrocytes. Therefore, this study demonstrates notable changes in hippocampal astrocytes, supporting the concept that these cells play an important role in PTSD symptomatology.

Antidepressant Effects of Ketamine Are Not Related to ¹8F-FDG Metabolism or Tyrosine Hydroxylase Immunoreactivity in the Ventral Tegmental Area of Wistar Rats.

Major depressive disorder (MDD) is an important health problem that is often associated to stress. One of the main brain regions related to MDD is the ventral tegmental area (VTA), a dopaminergic center, part of the reward and motivation circuitry. Recent studies show that changes to VTA dopaminergic neurons are associated with depression and treatment. Ketamine has recently shown a fast, potent antidepressant effect in acute, sub-anesthetic doses. Thus, our aims were to elucidate if ketamine would be able to revert depression-like behaviors induced by a chronic unpredictable stress (CUS) protocol and if it could cause alterations to metabolism and tyrosine hydroxylase (TH)-immunoreactivity in VTA. For this, 48 Wistar rats were divided into four groups: control + saline (CTRL + SAL), control + ketamine (CTRL + KET), CUS + saline (CUS + SAL), CUS + ketamine (CUS + KET). The CUS groups underwent 28 days of CUS protocol. Saline or ketamine (10 mg/kg) was administered intraperitonially once on day 28. The behavior was assessed by the sucrose preference test, the open field test, and the forced swim test. Glucose brain metabolism was assessed and quantified with microPET. TH-immunoreactivity was assessed by estimating neuronal density and regional and cellular optical densities. A decrease in sucrose intake in the CUS groups and an increase in immobility was rapidly reverted by ketamine (p < 0.05). No difference was observed in the open field test. There was no alteration to VTA metabolism and TH-immunoreaction. These results suggest that the depressive-like behavior induced by CUS and the antidepressant effects of ketamine are unrelated to changes in neuronal metabolism or dopamine production in VTA.

Astrocyte morphology after ischemic and hemorrhagic experimental stroke has no influence on the different recovery patterns.

Stroke, broadly subdivided into ischemic and hemorrhagic subtypes, is a serious health-care problem worldwide. Previous studies have suggested ischemic and hemorrhagic stroke could present different functional recovery patterns. However, little attention has been given to this neurobiological finding. Coincidently, astrocyte morphology could be related to improved sensorimotor recovery after skilled reaching training and modulated by physical exercise and environmental enrichment. Therefore, it is possible that astrocyte morphology might be linked to differential recovery patterns between ischemic and hemorrhagic stroke. Thus, we decided to compare long-term GFAP-positive astrocyte morphology after ischemic (IS, n=5), hemorrhagic (HS, n=5) and sham (S, n=5) stroke groups (induced by endothelin-1, collagenase type IV-S and salina, respectively). Our results showed ischemic and hemorrhagic stroke subtypes induced similar long-term GFAP-positive astrocyte plasticity (P>0.05) for all evaluated measures (regional and cellular optical density; astrocytic primary processes ramification and length; density of GFAP positive astrocytes) in perilesional sensorimotor cortex and striatum. These interesting negative results discourage similar studies focused on long-term plasticity of GFAP-positive astrocyte morphology and recovery comparison of stroke subtypes.

Body weight and percent body fat increase during the transition from high school to university in females.

A critical period for weight gain may occur during the transition from high school to university. This descriptive, noncontrolled cohort study of 116 healthy females examined the effect of this transition over three study visits in first year university. The main outcome measure was body weight; others were height, body composition, waist circumference, dietary intake, and participation in physical and sedentary activities. Difference among study visits was determined by repeated measures analysis of variance; multiple regression examined changes in energy intake and physical and sedentary activities as predictors of final weight. Weight increased (P<0.001) by 2.4 kg (61.4 to 63.8 kg) during the entire course of the study. Other increases (P<0.001) included: body mass index (calculated as kg/m(2)), 22.3 to 23.1; percent body fat, 23.8% to 25.6%; and waist circumference, 76.9 to 79.4 cm. Dietary energy intake did not increase; vigorous physical, but not strength building, activities increased; television use decreased; and computer use increased (P<0.03 for all): however, these changes were not predictive of final weight. A change (decrease) in moderate physical activity was, however, an important predictor of final weight. Females making the transition to university gained 2.4 kg; weight gain during this formative period may be modified by lifestyle activities.