RESUMO
AdC6gag37, an E1-deleted adenovirus recombinant derived from the chimpanzee adenovirus serotype 6 expressing a codon-optimized truncated form of gag of HIV-1, was tested for induction of transgene-specific CD8+ T cell responses upon intranasal or intravaginal immunization of mice. Administration of AdC6gag37 induced gag-specific CD8+ T cells at systemic and mucosal sites. Frequencies of gag-specific CD8+ T cells elicited in the genital tract by intravaginal or intranasal immunizations were substantially increased by intranasal priming followed by intravaginal boosting with the same vector. Additionally, intravaginal immunization with AdC6gag37 increased the amount of gammadelta T cells that could be detected in genital tract.
Assuntos
Vacinas contra a AIDS/administração & dosagem , Adenovirus dos Símios/genética , Linfócitos T CD8-Positivos/imunologia , Produtos do Gene gag/imunologia , Vetores Genéticos , Genitália Feminina/imunologia , Vacinas contra a AIDS/genética , Vacinas contra a AIDS/imunologia , Adenovirus dos Símios/imunologia , Administração Intravaginal , Animais , Feminino , Produtos do Gene gag/administração & dosagem , Produtos do Gene gag/genética , Infecções por HIV/imunologia , Infecções por HIV/prevenção & controle , HIV-1/imunologia , Imunidade nas Mucosas , Camundongos , Camundongos Endogâmicos BALB C , Receptores de Antígenos de Linfócitos T gama-delta/metabolismo , Recombinação GenéticaRESUMO
The aim of our study was to evaluate the benefits of supplementation with 800 mg/day of alpha-tocopherol with regard to cellular viability in HIV-1 seropositive patients undergoing anti-retroviral therapy. A total of 29 patients participated in the study, of whom 14 were given the supplement and 15 a placebo. The analyses were carried out before treatment commenced and after 60, 120 and 180 days. The plasma levels of HIV-1 RNA showed a significant decrease as a consequence of treatment time in the groups studied (p = 0.0001), although the difference between the treatments over time was not verified (p = 0.7343). The percentage of viable lymphocytes showed a significant increase as a consequence of treatment time in both groups studied (p = 0.0002) and a significant difference between the treatments over time (p = 0.0472). The percentage of lymphocytes in apoptosis showed a significant reduction over time (p = 0.0003), as well as a significant difference between the treatments over time (p = 0.0321). The significant increase in cellular viability indicates that supplementation with alpha-tocopherol offers an additional positive effect on cellular preservation in HIV-1 individuals undergoing anti-retroviral therapy; however, it represents an additional risk of anti-retroviral therapeutic failure, possibly due to drug-drug interaction involving up-regulation of metabolic clearance.
Assuntos
Fármacos Anti-HIV/uso terapêutico , Antioxidantes/uso terapêutico , Infecções por HIV/tratamento farmacológico , HIV-1/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , alfa-Tocoferol/uso terapêutico , Adulto , Fármacos Anti-HIV/sangue , Relação CD4-CD8 , Sobrevivência Celular/efeitos dos fármacos , Suplementos Nutricionais , Método Duplo-Cego , Quimioterapia Combinada , Feminino , HIV-1/genética , Humanos , Masculino , RNA Viral/sangue , alfa-Tocoferol/sangueRESUMO
O número absoluto de células CD34+ em sangue periférico permite predizer o momento ideal de coleta das células-tronco periféricas oferecendo segurança para a realização do transplante autólogo de medula óssea. A citometria de fluxo, através de um fluxo laminar, permite a análise e quantificação destas células baseada numa plataforma única. No presente trabalho foram considerados 62 pacientes submetidos a terapia de mobilização e quantificação de células CD34+, internados na Unidade de Transplante Medula Óssea do Hospital Governador Celso Ramos em Florianópolis. As análises foram feitas no Centro de Hematologia e Hemoterapia de Santa Catarina (HEMOSC).
