Is walking in daily life a moderate intensity activity in patients with chronic obstructive pulmonary disease?

BACKGROUND: So far there are no studies investigating whether the time spent walking in daily life by patients with COPD corresponds to an activity of at least moderate intensity according to the recommendations of the American College of Sports Medicine (ACSM). AIM: To quantify in patients with COPD the time spent/day in physical activity of at least moderate intensity according to the ACSM (TPA>moderate); and to compare their TPA>moderate with the total time spent walking/day in daily life (TW). DESIGN: Cross-sectional study. SETTING: Patients entering in a pulmonary rehabilitation program at a University Hospital in Brazil. POPULATION: Seventy-three patients with COPD (FEV1 40±15% pred; 65±9 years). METHODS: TW and TPA>moderate were respectively assessed with the DynaPort and SenseWear activity monitors for 12 hours/day during 2 days. RESULTS: There was significant difference between TW and TPA>moderate (53 [36-80] versus 12 [3-33] minutes/day, respectively; P<0.0001). Although 84% of patients reached more than 30 minutes/day of TW, only 29% reached more than 30 minutes/day of TPA>moderate. TPA>moderate correlated modestly with TW (r= 0.52; P<0.0001). CONCLUSION: The majority of patients with moderate-very severe COPD walk more than 30 minutes/day; however this does not mean that they are physically active, since less than 1/4 of their time spent walking can be considered as moderate-intensity activity. CLINICAL REHABILITATION IMPACT: Moderate-intensity physical activity in patients with COPD is more reduced than previously known. There is a clear need to increase their daily physical activity and lead a higher proportion of this population to comply with the minimum recommendations.

Development of a standard set of microsatellite reference alleles for identification of grape cultivars.

In order to investigate the comparability of microsatellite profiles obtained in different laboratories, ten partners in seven countries analyzed 46 grape cultivars at six loci (VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79). No effort was made to standardize equipment or protocols. Although some partners obtained very similar results, in other cases different absolute allele sizes and, sometimes, different relative allele sizes were obtained. A strategy for data comparison by means of reference to the alleles detected in well-known cultivars was proposed. For each marker, each allele was designated by a code based on the name of the reference cultivar carrying that allele. Thirty-three cultivars, representing from 13 to 23 alleles per marker, were chosen as references. After the raw data obtained by the different partners were coded, more than 97% of the data were in agreement. Minor discrepancies were attributed to errors, suboptimal amplification and visualization, and misscoring of heterozygous versus homozygous allele pairs. We have shown that coded microsatellite data produced in different laboratories with different protocols and conditions can be compared, and that it is suitable for the identification and SSR allele characterization of cultivars. It is proposed that the six markers employed here, already widely used, be adopted as a minimal standard marker set for future grapevine cultivar analyses, and that additional cultivars be characterized by means of the coded reference alleles presented here. The complete database is available at http://www.genres.de/eccdb/vitis/ Cuttings of the 33 reference cultivars are available on request from the Institut National de la Recherche Agronomique Vassal collection (didier.vares@ensam.inra.fr).