[Evaluation of substrate specificity of biosensor models based on strains degrading polycyclic aromatic compounds].

Models of microbial biosensors based on 11 strains of degrading surface-active substances (SASs) and polycyclic aromatic hydrocarbons (PAHs) were studied. Substrate specificity, sensitivity, and stability of biosensor models were comparatively evaluated.

[Comamonas testosteroni strain TI as a potential base for a microbial sensor detecting surfactants]. / Shtamm Comamonas testosteroni TI kak potentsial'naia osnova kletochnogo sensora dlia obnaruzheniia poverkhnostno-aktivnykh veshchestv.

Strain Comamonas testosteroni TI, capable of degrading the nonionic surfactant (NIS) nonylphenolethoxylate (OP-10), was used for constructing a pilot cellular biosensor. The lower NIS detection limit for the biosensor was 0.25 mg/l. We studied the substrate specificity of the biosensor with respect to a wide range of organic compounds: surfactants, polyaromatic compounds (PAC), carbohydrates, alcohols, etc. It was shown that the biosensor based on Comamonas testosteroni TI did not respond to glucose, which was an advantage over the formerly described biosensor based on Pseudomonas rathonis T. The amplitude of the sensor response remained stable for 10 days.