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Tissue Cell ; 44(5): 301-7, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22608000

RESUMO

The aim of this study was to identify and quantify the argyrophil, argentaffin and insulin-immunoreactive cells (IIC) in the small intestine of the opossum Didelphis aurita. Seven adult male specimens of opossums were investigated. The animals were captured, and their blood insulin levels were determined. After euthanasia, fragments of the small intestine were processed for light microscopy and transmission electron microscopy, and submitted to histochemistry and immunohistochemistry for identification of argyrophil and argentaffin endocrine cells, and IIC. Argyrophil and argentaffin cells were identified in the intestinal villi and Liberkühn crypts, whereas IIC were present exclusively in the crypts. Ultrastructure of the IIC revealed cytoplasmic granules of different sizes and electron densities. The numbers of IIC per mm(2) in the duodenum and jejunum were higher than in the ileum (p<0.05). The animals had low levels of blood insulin (2.8 ± 0.78 µIU/ml). There was no correlation between insulin levels and the number of IIC in the small intestine. The IIC presented secretory granules, elongated and variable morphology. It is believed that insulin secretion by the IIC may influence the proliferation of cells in the Liberkühn crypts, and local glucose homeostasis, primarily in animals with low serum insulin levels, such as the opossum.


Assuntos
Didelphis/metabolismo , Células Endócrinas/metabolismo , Células Endócrinas/ultraestrutura , Células Enterocromafins/metabolismo , Insulina/metabolismo , Intestino Delgado/metabolismo , Animais , Proliferação de Células , Grânulos Citoplasmáticos/ultraestrutura , Didelphis/imunologia , Células Endócrinas/citologia , Células Enterocromafins/citologia , Células Enterocromafins/ultraestrutura , Imuno-Histoquímica , Insulina/imunologia , Mucosa Intestinal/citologia , Mucosa Intestinal/ultraestrutura , Intestino Delgado/ultraestrutura , Masculino , Microscopia Eletrônica de Transmissão , Gambás/metabolismo
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