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1.
Arq. bras. med. vet. zootec. (Online) ; 73(6): 1334-1345, Nov.-Dec. 2021. tab, graf, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1355678

RESUMO

The present work evaluated the immunomodulatory effect of thalidomide (Thal) at different doses on tumor-associated macrophages (TAMs) using a mouse model of human breast cancer. Mice were inoculated with 4T1 cells in the left flank and treated with Thal once a day at concentrations of 50, 100, and 150mg/kg body weight from the 5th day until the 28th day of tumor inoculation. The tumors were sized, proliferation index and TAMs count were evaluated in primary tumors and metastatic lungs. In addition, the metastasis rate was evaluated in the lungs. Thal at 150mg/kg significantly decreased tumor growth, proliferation index, and TAMs infiltration in primary tumors. Conversely, a higher number of TAMs and lower proliferation index were observed in metastatic lungs in mice treated with 150mg/kg of Thal. Furthermore, Thal at 150mg/kg significantly decreased the metastatic nodules in the lungs. Our findings demonstrated that Thal treatment considerably decreased the primary tumor and lung metastasis in mice associated with different TAM infiltration effects in these sites.(AU)


No presente trabalho, foi avaliado o efeito imunomodulador de diferentes doses de talidomida em macrófagos associados ao tumor (TAMs), em um modelo murino de câncer de mama. Camundongos foram inoculados com células 4T1, na região do flanco esquerdo, e tratados com talidomida, uma vez ao dia, nas doses de 50, 100 e 150mg/k, por massa corporal, do quinto dia ao 28º dia de inoculação tumoral. Os tumores foram medidos, o índice de proliferação celular e a contagem de TAMs foram avaliados nos tumores primários e nos pulmões com metástases. Além disso, a taxa de metástases pulmonares também foi avaliada. A talidomida na dose de 150mg/kg diminuiu significativamente o crescimento tumoral, o índice de proliferação celular e a infiltração de TAMs nos tumores primários. Por outro lado, maior número de TAMs e menor índice de proliferação celular foram observados nos pulmões metastáticos, em camundongos tratados com 150mg/kg de talidomida. Ademais, a talidomida na dose de 150mg/kg diminuiu significativamente os nódulos metastáticos nos pulmões. Os resultados demonstraram que o tratamento com talidomida diminuiu o crescimento tumoral e as metástases pulmonares em camundongos, associado com diferentes efeitos na infiltração de TAMs nesses locais.(AU)


Assuntos
Animais , Camundongos , Talidomida/análise , Neoplasias Mamárias Animais/tratamento farmacológico , Macrófagos/efeitos dos fármacos , Imunomodulação , Metástase Neoplásica
2.
Pharmazie ; 74(4): 221-226, 2019 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-30940305

RESUMO

The use of sirolimus and its analogs has been evaluated in studies aimed at combating several types of cancer; however, because of the limited bioavailability of the drug, the search for new forms of administration is required. Biodegradable polymeric implants containing sirolimus were developed and assessed as an alternative method of drug administration. Implants containing 25 % (w/w) sirolimus were prepared employing the polymer matrices chitosan, polycaprolactone and poly(lactic-co-glycolic acid) (PLGA) in two proportions: PLGA 50:50 and PLGA 75:25. Thermal analysis techniques such as thermogravimetry and differential scanning calorimetry, combined with x-ray diffraction were used to characterize and evaluate the compatibility of the constituents of the formulation. No incompatibilities were found between the components, but drug amorphization was observed in all samples. Implants made from the polymers chitosan and PCL may accelerate the degradation of SRL when these polymers are dissolved in methanol at 50 °C. HPLC analysis showed that the implant prepared with PLGA 75:25 did not present degradation products and maintained its appropriate drug content, even when dissolved in methanol and heated to 50 °C. Therefore, it represents the most suitable biodegradable polymer for use in implants developed for the treatment of malignant solid tumors. However, it is still necessary to further study the drug effects after amorphization of the crystal and also to perform stability and solubility analysis.


Assuntos
Antineoplásicos/administração & dosagem , Portadores de Fármacos/química , Polímeros/química , Sirolimo/administração & dosagem , Varredura Diferencial de Calorimetria , Química Farmacêutica/métodos , Quitosana/química , Cromatografia Líquida de Alta Pressão/métodos , Implantes de Medicamento , Poliésteres/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Sirolimo/química , Temperatura , Termogravimetria , Difração de Raios X
3.
J Fr Ophtalmol ; 40(3): 202-208, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28256268

RESUMO

PURPOSE: To evaluate the antiangiogenic activity of bevacizumab-loaded polyurethane using two animal models of neovascularization. METHODS: The percentage of blood vessels was evaluated in a chicken chorioallantoic membrane model (n=42) and in the rabbit cornea (n=24) with neovascularization induced by alkali injury. In each model, the animals were randomly divided into the groups treated with the bevacizumab-loaded polyurethane device, phosphate-buffered-saline (negative control) and bevacizumab commercial solution (positive control). Clinical examination, as well as histopathological and immunohistochemical evaluation, were performed in the rabbit eyes. Microvascular density in hot spot areas was determined in semi-thin sections of corneal tissue by hematoxylin-eosin staining and factor VIII immunohistochemistry. Immunohistochemical analysis was also performed to evaluate VEGF expression. RESULTS: In the evaluated models, the use of bevacizumab (Avastin®) and the bevacizumab-loaded polyurethane device led to similar results with regard to inhibition of neovascularization. In the chorioallantoic membrane model, the bevacizumab-loaded polyurethane device reduced angiogenesis by 50.27% when compared to the negative control group. In the rabbit model of corneal neovascularization, the mean density of vessels/field was reduced by 46.87% on analysis of factor VIII immunohistochemistry photos in the bevacizumab-loaded polyurethane device group as compared to the negative control (PBS) sections. In both models, no significant difference could be identified between the bevacizumab-loaded polyurethane device and the positive control group, leading to similar results with regard to inhibition of neovascularization. CONCLUSIONS: The present study shows that the bevacizumab-loaded polyurethane device may release bevacizumab and inhibit neovascularization similarly to commercial bevacizumab solution in the short-term.


Assuntos
Inibidores da Angiogênese/administração & dosagem , Bevacizumab/administração & dosagem , Neovascularização da Córnea/tratamento farmacológico , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos/métodos , Poliuretanos , Animais , Embrião de Galinha , Neovascularização da Córnea/patologia , Modelos Animais de Doenças , Composição de Medicamentos , Sistemas de Liberação de Medicamentos/instrumentação , Avaliação Pré-Clínica de Medicamentos , Implantes de Medicamento/química , Feminino , Poliuretanos/química , Coelhos
4.
Pharmazie ; 71(8): 439-446, 2016 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-29442030

RESUMO

Post-operative endophthalmitis is an infection and an inflammation of the eye following a surgical procedure. Its treatment is based on drug injections into the eye. However, this treatment can lead to ocular complications. Intraocular implants could substitute the conventional therapy. Poly(lactic-co-glycolic acid) (PLGA) implants comprising on vancomycin and dexamethasone were evaluated as drug delivery system to treat endophthalmitis after cataract surgery. Implants were characterized by drug content uniformity, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Wide Angle X-ray Scattering (WAXS), Scanning Electron Microscopy (SEM) and in vitro drug release. The bactericidal effect of vancomycin, eluted from the implants, was demonstrated against Staphylococcus aureus and Staphylococcus epidermidis. The drugs were uniformly distributed in the polymer. The analytical techniques revealed the chemical integrity of the drugs incorporated into the polymer and the modification of dexamethasone semi-crystalline nature. Drugs were controlled released from implants; and the eluted vancomycin showed bactericidal effects. In conclusion, PLGA implants containing vancomycin and dexamethasone may represent a therapeutic alternative to treat post-operative endophthalmitis.


Assuntos
Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/uso terapêutico , Bactérias/efeitos dos fármacos , Dexametasona/administração & dosagem , Dexametasona/uso terapêutico , Portadores de Fármacos , Ácido Láctico , Ácido Poliglicólico , Infecção da Ferida Cirúrgica/prevenção & controle , Vancomicina/administração & dosagem , Vancomicina/uso terapêutico , Antibacterianos/farmacologia , Implantes de Medicamento , Liberação Controlada de Fármacos , Endoftalmite/microbiologia , Endoftalmite/prevenção & controle , Humanos , Testes de Sensibilidade Microbiana , Procedimentos Cirúrgicos Oftalmológicos , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus epidermidis/efeitos dos fármacos , Vancomicina/farmacologia
5.
Pharmazie ; 68(4): 235-9, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23700787

RESUMO

An HPLC-UV method was developed and validated for the determination of acyclovir in vitreous humor. The method was carried out in isocratic mode using 0.02 mol/L acetic acid/methanol (95:5) as mobile phase, a C18 column at 25 degrees C and UV detection at 254 nm. The method was linear (r2> 0.99) over the range of 35-700 microg/mL, precise (RSD <5%), accurate (recovery ranged from 98.18 to 99.64%), robust, selective regarding of the vitreous humor, and robust remaining unaffected by deliberate variations in relevant parameters. The validated HPLC-UV method can be successfully applied to determine acyclovir directly injected into the vitreous cavity of rabbits' eye.


Assuntos
Aciclovir/análise , Antivirais/análise , Corpo Vítreo/química , Animais , Cromatografia Líquida de Alta Pressão , Indicadores e Reagentes , Limite de Detecção , Coelhos , Padrões de Referência , Reprodutibilidade dos Testes , Síndrome de Necrose Retiniana Aguda/tratamento farmacológico , Soluções , Espectrofotometria Ultravioleta
6.
Int J Pharm ; 372(1-2): 17-23, 2009 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-19162152

RESUMO

Thalidomide presents polymorphism and is a problematic drug due to its poor solubility and difficult tablet processability, which is the dosage form available in Brazil. In most cases, the pharmacopoeias specify do not address solid state characterization of drugs precisely. In this work, different thalidomide commercial samples were characterized by infrared spectroscopy, particle size analysis, scanning electron microscopy, and X-ray diffraction. In addition, the polymorphic forms were quantified for Rietveld analysis and their intrinsic dissolution rates were evaluated. The results demonstrated the market availability of different raw materials which lack of homogeneity due to differences related to crystalline constitution, crystal habit and intrinsic dissolution rate.


Assuntos
Talidomida/química , Avaliação Pré-Clínica de Medicamentos/métodos , Tamanho da Partícula , Solubilidade , Talidomida/análise , Difração de Raios X/métodos
7.
Curr Eye Res ; 31(6): 525-34, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16769612

RESUMO

The treatment of vitreoretinal diseases is limited and, nowadays, new drug delivery approaches have been reported in order to increase drug bioavailability. The objective of the current study was to determine the pharmacokinetic profile of a biodegradable dexamethasone acetate implant inserted into the vitreous of rabbits and to evaluate its potential signs of toxicity to the rabbits' eyes. The results showed that the intravitreous drug concentration remained within the therapeutic range along the 8-week period of evaluation. The system under study was not toxic to the normal rabbit retina, and no significant increase in intraocular pressure was observed.


Assuntos
Dexametasona/análogos & derivados , Glucocorticoides/farmacocinética , Retina/metabolismo , Corpo Vítreo/metabolismo , Implantes Absorvíveis , Animais , Disponibilidade Biológica , Dexametasona/farmacocinética , Dexametasona/toxicidade , Implantes de Medicamento , Eletrorretinografia/efeitos dos fármacos , Glucocorticoides/toxicidade , Masculino , Coelhos , Retina/efeitos dos fármacos , Corpo Vítreo/efeitos dos fármacos
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