[Isolation of a Pasteurella multocida bacteriophage]. / Isolierung eines Pasteurella-multocida-Bakteriophagen.

Lysogenesis testing of 84 Pasteurella multocida strains resulted in detection of one lysogenic strain from which phage B225 was isolated. B225 lysed only one Pasteurella multocida strain isolated from cattle. The Pasteurella phage, when examined by electron microscopy, showed a hexagonal head and a non-contractile, Flexible tail, It could be attached, consequently, to Group B according to Bradley (1967).

[Immunoprophylactic methods of prevention of pneumopathies in young cattle]. / Immunoprophylaktische Methoden zur Vorbeugung der Pneumopathien bei Jungrindern.

The bivalent Pasteurella vaccine used to immunise young cattle is an anaculture adsorbed to aluminium hydroxide and consisting of Pasteurella multocida and Pasteurella haemolytica serotypes which could be isolated; most often from pneumonically changed lungs and nasal mucus. It was administered together with trivalent antiviral vaccine and reduced by 50 per cent the loss rate due to mortality and emergency slaughtering. Good protection was obtained from applying the first vaccination dose on the supplier's farm.

[Identification of Pasteurella serotypes by indirect hemagglutination]. / Identifizierung der Pasteurellaserotypen durch indirekte Hämagglutination.

The indirect haemagglutination test was used for serological type identification of Pasteurella strains. The Pasteurella multocida strains were tested by parallel application of antigen production methods (common method plus hyaluronidase treatment). Thirty-nine (72.2 per cent) of 54 Pasteurella multocida strains, which had been obtained from cattle and swine, were serologically tested by the common technique of antigen production, their types being identified, while 42 (77.7 per cent) were tested and typed by means of hyaluronidase treatment. Depolimerisation of hyaluronic acid by means of hyaluronidase increased the agglutinability of eight strains (14.8 per cent) which could thus be identified by types. Serological identification of 47 (87.1 per cent) Pasteurella multocida strains was achieved by parallel use of the two antigen production methods. Identification was not possible of two strains. Cross-reaction was recorded from five strains. Most of the Pasteurella multocida strains isolated from cattle were A serotype (54 per cent), while those of swine were B strains (50 per cent). Thirty-six of 40 serologically tested Pasteurella haemolytica strains were serotypes A1, A2, T3, T4, A5, and A6, and most of these were A5 and T4.

Pasteurella infections in respiratory diseases of young cattle.

Bacteriological examinations performed on 545 extracts of pneumonia-affected lungs and on nasal secretions collected from pneumopathy diseased young cattle led to the isolation of 176 (32.2%) Pasteurella (p.) multocida strains and of 125 (22.9%) P. haemolytica strains. P. multocida was isolated in 46.2% of the lung lesions and in 22.5% of the nasal secretions, P. haemolytica in 26.6% and 20.3%, respectively. Pathogenicity in mice was detected in 81.9% of the P. multocida strains and in 19% of the P. haemolytica strains. Pasteurella strains were very susceptible to chloramphenicol (82.3%), tetracycline (68%), penicillin (47%), and ampicillin and resistant to oxacillin, streptomycin and kanamycin. P. multocida and P. haemolytica strains in the respiratory tract and lung lesions of young cattle affected by pneumopathies represent the main ethiologic agent accounting for the clinical symptoms, anatomic lung lesions and finally for the death of the animal.