Detection in a murine T lymphoma of a lipid-like factor that inhibits cell growth.

In this paper we show that MCG3 cells, a murine T lymphoma, contain a factor(s) that inhibits the proliferation of cells of different histological origin. The lack of sensitivity of this cell proliferation-inhibiting factor (CPIF) to the treatment with proteolytic enzymes and its solubility in organic solvent demonstrated that it is a lipid-like substance. Separation by thin-layer chromatography showed it migrates before the prostaglandins with activity on cell proliferation. CPIF activity was reversible and more intense on bone marrow cells than on tumor cells, suggesting that it can play a role in cell growth regulation.

[Study and characterization of lipid factors that inhibit cellular proliferation in MCG3 rat T lymphoma]. / Estudio y caracterización de factores lipídicos inhibidores de la proliferación celular (FLIPC) en el linfoma T de ratón MCG3.

Lipid factors that inhibit cellular proliferation have been detected in MCG3 cells, a T lymphoma of B10 mouse. The inhibiting effect is cytostatic, non cytotoxic, reversible and lacks species and tissue-specificity. These lipids have been isolated by Thin Layer Chromatography (chloroform:methanol:water, 60:38:8) showing an Rf100 between 36 and 42, similar to that shown by gangliosides and phosphatidylinositol.