Effect of Entomopathogenic Fungi to Eggs and Nymphs Survival of Halyomorpha halys (Hemiptera: Pentatomidae) Under Laboratory Conditions.

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is becoming a major pest of Greece's agricultural production, including tree fruit. Until now, the control of this pest has been based on conventional methods, with the application of chemical insecticides being the primary option. However, the wide distribution of H. halys, in combination with the large area of tree fruit production in Greece, raise the need for alternative control methods. In this study, we investigated the possibility of implementing microbial control agents for managing H. halys. Eggs and nymphs of H. halys were treated with 15 native entomopathogenic fungal isolates and their virulence was evaluated in the laboratory. After treatment, egg hatching time was recorded for 7 days and ranged from 4.5 to 7.0 days. Nymphal survival was recorded daily for 7 days following application and ranged from 2.1 to 6.6 days for second instar nymphs and 3.7 to 6.8 days for fourth instar ones. Beauveria varroae isolate displayed the highest toxicity to all H. halys stages that were tested and could be considered a promising biocontrol agent of this insect. Please check and confirm that the authors and their respective affiliations have been correctly identified and amend if necessary.οκ.

Age- and Density-Dependent Parasitism Rate and Development Time of the Generalist Egg-Parasitoid Ooencyrtus telenomicida (Hymenoptera: Encyrtidae) on Eggs of the Brown Marmorated Stink Bug, Halyomorpha halys.

Halyomorpha halys (Hemiptera: Pentatomidae) is an invasive pest species that was imported into Greece in 2011 and since then, has caused severe qualitative and quantitative damage to economically important crops. Its management relies mainly on the use of broad-spectrum insecticides, with little to no information available concerning the potential use of native parasitoids in terms of classical biological control. Our study aimed to assess the parasitism rate and development time of the gregarious egg parasitoid Ooencyrtus telenomicida (Hymenoptera: Encyrtidae) on H. halys egg-masses, depending on several factors such as: (i) age of parasitoids, (ii) density of parasitoids, (iii) age of host eggs, and (iv) oviposition experience of parasitoids. According to our results, the younger the host eggs and the more parasitoids, the higher the parasitism rate achieved by adults of O. telenomicida, with the maximum mean value of the parasitism rate observed with 1-day-old host eggs and 4 parasitoid pairs (57.3%). On the contrary, the lowest mean value of the parasitism rate was observed with 4-day-old host eggs and 1 parasitoid pair (6.5%). Similarly, the age of parasitoids significantly affected parasitisation. The older the parasitoids were, the higher the parasitism rate achieved by adults of O. telenomicida, with a three-fold higher parasitism rate observed at 3-4 and 5-6-day-old O. telenomicida, compared to 1-2-day-old (31.8, 32.4, and 12.1%, respectively). Individuals that developed in younger host eggs displayed a shorter development time, and the shortest development time was observed for O. telenomicida laid by 2 parasitoid pairs. Parasitoid age did not affect the development time of O. telenomicida, although there was a tendency for individuals laid by younger female parasitoids to exhibit a shorter development time. Our findings provide valuable information on the potential use of O. telenomicida as a biocontrol agent of H. halys.

First Report of Native Parasitoids of Halyomorpha halys (Hemiptera: Pentatomidae) in Greece.

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an endemic species of East Asia; it was introduced into Europe in 2007. It has a wide range of hosts as it feeds on over 170 host plant species and significantly impacts crop production. In Greece, H. halys causes significant losses in the production of kiwi, peaches, and green beans; thus, control of this species (including biological control) is essential. Here, we focus on the potential impact of native natural enemies of H. halys in Greece. From June to October 2020, we sampled naturally field-laid H. halys egg masses to recover native parasitoids. A total of 20 egg masses of H. halys were collected from infested fields from different locations in northern Greece. Out of 529 eggs, 45 parasitoids managed to hatch successfully. The overall parasitism rate was 8.5%. We found two species of Hymenopteran egg parasitoids attacking H. halys eggs-Anastatus bifasciatus (Geoffrey) (Hymenoptera: Eupelmidae) and Ooencyrtus telenomicida (Vassiliev) (Hymenoptera: Encyrtidae), with the former comprising 58% of all parasitoids that were recovered. These results contribute to the knowledge about the natural enemy community that attacks H. halys in Greece, and the use of these native egg parasitoids in biological control programs may be a viable H. halys management strategy.