RESUMO
Ultra-high molecular weight polyethylene (UHMWPE) is a prevailing bearing material applied in joint arthroplasty. Despite not being a novel biomaterial, its debris as consequence of long application and surface properties usually still lead to short lifespan. Many of the drawbacks are associated with sterilization methods that degrade the surface properties of UHMWPE. This work aims at improving the sterilizing treatment and also increasing material wettability, without losing bulk properties, which are essential for an orthopedic bearing. Cold plasma in hollow cathode setting was used for the material surface functionalization. Samples were characterized through contact angle (WCA), x-ray diffraction (XRD), optical microscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and profilometry. Optimal points based on immediate surface wettability, shelf time and sterilization efficacy were chosen for biocompatibility evaluation. When comparing cell viability through MTT among treated samples (OP1, OP2 and UV), a slight reduction in OP2 viability could be seen after 7â¯days incubation, which is also observed in Giemsa staining and SEM images. In late incubation, OP1 loses its hydrophilic character and displays higher cell adhesion than its counterparts UV and OP2. At the end, OP2 showed less cells growing over the biomaterial after 7â¯days exposition compared to OP1 and UV. OP1 presented a more hydrophobic surface and improved cell adhesion, differently from OP2 and UV, which maintained their wettability conditions in late incubation. Cell analysis results indicate that surface wetting influences cell morphology and consequent cell adhesion, in which more hydrophobic surfaces are shown to favor fibroblast adhesion properties.
