Frequency of occurrence of metabolic syndrome risk factors in children and adolescents from the city of Wroclaw and surroundings

Background: A recently growing number of children and adolescents with overweight or obesity is indicative of the need for diagnosing their complications that may appear in the early childhood. For this reason, diagnostic criteria were developed for components of the metabolic syndrome (MS) also for these groups of the population. Objective: This study was aimed at evaluating the frequency of metabolic syndrome risk factors occurrence in children and adolescents from the city of Wroclaw and surroundings depending on gender, age and physical activity. Material and methods: Investigations on the frequency of occurrence of dietary and non-dietary risk factors of the metabolic syndrome were carried out in the years 2010-2017 among 771 children and adolescents aged 10-18 year, attending to primary schools, gymnasiums and secondary schools in Wroclaw. Results: The lack of any components of the metabolic syndrome was demonstrated in 14.78% of the children aged 10-12 years as well as in 17.38% of both adolescents aged 13-15 and 17-18 years. One risk factor was most frequently diagnosed in children aged 10-12 years (17.89%) and it was arterial hypertension (16.08% of the whole surveyed population). Three MS components were demonstrated in 15 persons (1.95% of the whole surveyed population), including in 7 girls and 8 boys. The persons with three MS risk factors from the age category 13-15 years constituted 0.26% whereas these from the age category 17-18 years constituted 1.69% of the whole surveyed group. Conclusions: It shall be concluded that the incidence of the fully symptomatic MS depended significantly on the age of the surveyed, but not on their gender. Among the three adopted components of MS, the most frequently demonstrated disorders included: arterial hypertension, abdominal obesity and increased concentration of triglycerides in blood serum.

Effect of addition of dried potato pulp on selected quality characteristics of shortcrust pastry cookies.

Potato pulp is a nuisance waste from the production of potato starch, containing large amounts of dietary fiber; therefore, addition of the pulp to food may have a positive effect on the nutritional value of food products. To increase the amount of dietary fiber, shortcrust pastry cookies were baked by replacing some of the flour (20-100%) with an equivalent amount of dried potato pulp. In all the tested variants, correct confectionery products were obtained. The color of finished product (measured using Konica-Minolta CM-5 spectrophotometer) and mechanical properties of raw dough and baked cookies (subjected to a tensile test, using INSTRON 5544 Tensile Tester) were determined. Furthermore, the samples were subjected to sensory consumer assessment (evaluated on a 7-point hedonic scale). Colorimetric tests of the cookies showed a slight shift in their color from yellow toward green but at the same time lowering its saturation. Strength tests show that only the replacement of more than 40% of flour by the dried pulp resulted in a significant increase in the hardness of baked products. Regarding the tests with consumers, cookies containing up to 40% potato pulp were acceptable. Such a large addition of potato pulp to shortcrust pastry cookies creates new opportunities for this waste management.

Influence of arsenic on selected biochemical blood parameters in rats fed diet with different fat and protein content.

BACKGROUND: Arsenic is widely distributed in the environment. The main routes of absorption of inorganic arsenic compounds are the lungs and the gastrointestinal tract. Arsenates both (III) and (V) are absorbed from the gastrointestinal tract in 55-95%, while the organic arsenic compounds in 75-85%. Arsenic poisoning leads to damage the activities and morphological changes in the stomach and intestines, causing the occurrence of nausea, vomiting and diarrhoea. Arsenic compounds may also be the cause of the development of certain cancers (lung, skin and liver). The first changes caused by arsenic poisoning usually remain unnoticed. Arsenic affects haematological and both lipid and carbohydrate metabolism. It also causes changes in the organs involved in metabolism, so biochemical parameters or enzymes activity are therefore a good indicator of poisoning changes. OBJECTIVE: The aim of this study was to examine the influence of protein and fat content in diet on selected biochemical blood parameters in rats. MATERIAL AND METHODS: Rats (11 groups n = 88) were fed with 5 types of diet: control, low-protein, high-protein, low-fat and high-fat. Animals received water without arsenic (control group) or water with 10 or 20 µg As/mL. RESULTS: In animals fed a low protein diets, regardless of the dose of arsenic, it was a decreasing of total cholesterol, triglycerides, and glucose in serum observed, compared to the control group. In the groups fed with low-protein diet revealed a significantly less damage in the liver as compared to the control group. In animals fed high-protein diets and with varying addition of arsenic a significant higher concentration of various biochemical parameters were found, in comparison to the respective control groups. In animals fed the high protein diet and poisoned with 20 µg As/mL of the arsenic significantly higher liver damage were found, compared to control group. CONCLUSIONS: Symptoms of arsenic hepatotoxicity measured with enzyme activity were highest in the groups of animals fed with low-protein diet. The parameters of lipid and carbohydrate metabolism depended mostly on diet than the dose of arsenic.

Arsenic contents in rats' fur as an indicator of exposure to arsenic. Preliminary studies.

BACKGROUND: Since arsenic compounds have an affinity to thiol groups their greatest amounts can then be found in the tissues containing sulphur-rich proteins, like beta-keratin in skin, hair and nails. Accumulation of arsenic also depends on the macronutrient content in daily food ration. The deficiency and excess of both the protein and fat may contribute to a higher content of arsenic in the organism, including hair in human or fur in animals. OBJECTIVE: Hair and fur is a good indicator of population exposure to many toxic substances, including arsenic. The degree of arsenic accumulation may depend on the diet and nutritional status. The aim of this study was to determine the effect of protein and fat in diet on the accumulation of arsenic in rats' fur. MATERIALS AND METHODS: A total number of 70 male Buffalo rats (body weight 200 - 220 g, age - 6 weeks) were divided into 10 groups. Rats were housed in plastic cages (4 per cage) in a 12h light/dark cycle for 6 weeks. The diets of different protein and fat contents ware administered to the animals. Five of ten groups of rats received throughout the whole period 10 ppm sodium arsenite dissolved in distilled drinking water (about 250 µg As/animal/day). The arsenic were determined with the method of atomic adsorption spectrometry in conjunction with a graphite-furnace atomize using a Varian AA240FS apparatus. RESULTS: The highest arsenic concentrations were found in fur of rats which were given low protein diet and water with arsenic. The lowest arsenic contents were found in fur of rats, which were given control diet and high protein diet with arsenic in water. CONCLUSIONS: Balanced control diet or high protein diet protected organism from arsenic accumulation, only small increase of arsenic content in rats' fur, compared to the control group, was observed. KEY WORDS: arsenic, rats' fur, protein and fat in diet, exposure to arsenic.

[Nutrition evaluation of 13 years old adolescents from a little town]. / Sposób zywienia 13-letniej mlodziezy z malego miasta.

Food intake and Body Mass Index (BMI) as basic indicator of nourishment of adolescents from a little town in Wielkopolska were evaluated. Triple 24 hour recall was conducted in group of 30 girls and 30 boys 13 years old. Quality and quantity of food rations consumed was evaluated. Energy and 20 nutrient in ratios were assessed. Mean food rations were compared with dietary standards for such adolescents in Poland. It was found that investigated group of adolescents has improper eating habits resulting unsatisfactory low energy, macronutrients, vitamins and minerals intake. Results of anthropometric measurements have shown right body mass in 40% of adolescents, malnutrition in 37%, and tendency to overweight and obesity in 20% of boys and girls.

Evaluation of plant products from the Legnicko-Glogowski region for their contamination with arsenic.

Contents of arsenic were determined in plant products originating from the region of two copperworks, Glogów and Legnica. Analyses were carried out by means of atomic absorption spectrometry, using an MHS-10 unit for hydride generation (acetylene/argon), after wet mineralisation of samples. The maximum permissible level of arsenic was not exceeded in any of the examined samples of cereals, potatoes, carrots, beetroots, cabbages, tomatoes, apples and pears, originating from the regions under scrutiny.

[The influence of RS4 resistant starch on wistar rats metabolism. Biochemical and lipid indices]. / Wplyw skrobi opornej RS4 na metabolizm szczurów rasy Wistar. Wskazniki biochemiczne i lipidowe.

The influence of resistant starch RS4 on total cholesterol (TC), HDL cholesterol, triacyloglycerols (TAG) composition in blood serum and liver of rats was determined. 4 week experiment involved 32 males and 40 females laboratory Wistar rats allotted in 4 groups in each sex. Control rats were feed ad libitum with standarised synthetic diet AIN-93. In experimental groups animals were given modified feed enriched with 5% of resistant starch. monophosphate of potato starch, monophosphate of soluble potato starch and monophosphate of potato starch heated with glycne and microwaved were examined. Diet enrichment with resistant starch decerased triacyloglycerol level (TAG) while the total cholesterol (TC) level, in serum was not affected.

[Effects of resistant starch RS4 on magnesium and iron absorption in Wistar rats]. / Wplyw skrobi opornej RS4 naabsorpcje magnezu i zelaza u szczurów rasy Wistar.

The effect of resistant starch RS4 on apparent absorption of magnesium and iron was studied in Wistar rats. The rats (4 groups male n=12 and 4 groups female n=12) were fed for 4 weeks diets: control with wheat starch (K) and 3 diets with modified resistant starches (S1, S2, S3). After an adaptation period (14 d), rats were transferred to metabolic cages. Dietary intake and faeces were monitored for 3 days. Mg and Fe levels were assessed in diets and feces by atomic absorption spectrometry. Apparent absorption of minerals was calculated as mineral intake minus fecal excretion and expressed as persentage of intake. Our results confirmed that Mg apparent absorption in female rats fed diet with resistant starch S2 was significantly increased (+37%) compared with the control group.