[Impairments of rats' behavioral reactions, concentration of corticosterone and expression of cytokine's gene under experimental postviral fatigue].

The aim of the main research was investigated of interaction of neural, endocrine and immune systems under experimental postviral fatigue, behavioral reactions, level of corticosterone, changes of IL-3 and IL-10 gene expression in rats' hypothalamus and INF-α in hypothalamus and spleen were analyzed. It has been shown the decrease of physical activity, increasing of corticosterone's level and gene expression of cytokines after injection of Poly I:C as a model of postviral fatigue. After remedication of Poly I:C increasing of physical activity was shown.

Stress-induced changes in the functional activity of the neuroendocrine system: the modulatory activity of derinat.

Changes in the functional activity of the hypothalamo-hypophyseal-adrenocortical system (HHACS) and hypothalamo-hypophyseal-gonadal system (HHGS) in conditions of different levels of stress and the possibility of correcting them using the native DNA formulation Derinat, which has immunomodulatory properties, were studied. The vectors of changes in hormonal reactions in both systems were found to be independent of the intensity of stress: there were increases in corticosterone levels and decreases in testosterone concentrations in different stress models. Intraperitoneal administration of Derinat at doses of 10 and 50 mg/kg increased HHACS and HHGS activity and reversed the stress-induced reduction in the blood testosterone concentration, which may be evidence that Derinat has stress-protecting effects. Administration of Derinat promoted normalization of stress-induced changes in the production of immunomodulatory cytokines among lymphocyte-activating factors with regulatory influences not only on the functioning of the immune system, but also on the HHACS and HHGS functions.

Activity in the hypothalamo-hypophyseal-adrenocortical system on experimental induction of chronic fatigue syndrome.

Changes in the activity of the hypothalamo-hypophyseal-adrenocortical system (HHACS) were studied in an experimental model of chronic fatigue syndrome induced by i.p. administration of synthetic doublestranded RNA (polyriboinosinic:polyribocytidylic acid, Poly I:C) at a dose of 3 mg/kg. Functional changes in the different components of the HHACS were detected using standard tests with i.p. ACTH or hydrocortisone on the background of cold stress and injections of Poly I:C. Single doses of Poly I:C were followed by the development of impairments to HHACS function, with decreases in the ACTH sensitivity of adrenal cells and suppression of the negative feedback mechanism, resulting in significant decreases in corticosterone concentrations in standard tests with administration of ACTH and hydrocortisone.

[Stress-induced changes in functional activity of the neuro-endocrine system: modulatory action of derinat].

Changes in functional activity of HPA and HPG axes under stress influences of different intensity, and possible ways for their correction by native DNA preparation: derinat, possessing immune modulator effects, were studied. It was shown that the vector of changes in hormone's reactions of both axes did not depend on the intensity of stress influences: different models of stress increased corticosterone level and decreased testosterone level in rats' blood. Intraperitoneal injection of 10 and 50 mg/kg BW doses of Derinat to rats enhanced HPA and HPG axes activity, reversed stress-induced decrease of testosterone concentration in blood, that may indicate a stress-protective effect of derinat. Injection of derinat caused normalizing of stress-induced changes in immunomodulatory cytokines production within Lymphocyte Activating Factors, which regulate not only the immune system functions but also the functions of HPA and HPG axes.

[Activity of hypotnalamic-pituitary-adrenal axis by induction of experimental chronic fatigue syndrom].

Changes in the activity of hypothalamic-pituitary adrenal (HPA) axis were investigated in experimental model of chronic fatigue syndrome (CFS) induced by intraperitoneal administration of synthetic double-stranded RNA (polyriboinosinic: polyribocytidylic acid, Poly I : C) to rats in the dose of 3 mg/kg body weight. In order to reveal functional changes in different links of the HPA axis, standard probes with intraperitoneal administration of ACTH and hydrocortisone against the background of cold stress application and Poly I : C injections were performed. A single injection of Poly I : C led to disordered HPA axis functions which was manifested by decreased sensitivity of the cells in the adrenal gland in response to ACTH, and suppression of the mechanism of negative feedback resulting in significant fall of cortisocterone concentration in standard assays with ACTH and hydrocortisone administration.