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1.
Lab Chip ; 23(24): 5120-5130, 2023 12 05.
Artigo em Inglês | MEDLINE | ID: mdl-37937378

RESUMO

In recent decades, the rise of ß-lactamases has substantially led to the emergence and wide spread of antibiotic resistance posing a serious global health threat. There is growing need for the development of rapid, cost-effective and user-friendly diagnostic assays for the accurate detection of ß-lactamases to optimize patient outcomes and prevent the spread of multidrug-resistances. In this article, we present a poly-dimethylacrylamide (PDMA)-based surface functionalization to immobilize ß-lactam antibiotics and ß-lactamase inhibitors of different subclasses. Immobilization was induced via UV-crosslinking through C,H-insertion reactions. The functional coatings were successfully applied in a highly efficient assay for the determination of recombinant ß-lactamases as well as ß-lactamases isolated from clinically relevant bacterial strains. Thus, this method describes an innovative approach with several significant benefits for diagnostic applications: the creation of specific detection platforms tailored for ß-lactamase activity, the development of high-throughput diagnostic assays and benefits regarding stability and shelf-life. Furthermore, this method is highly adaptable to other surfaces, antibiotics, and analytes, offering far-reaching implications for various biomedical, environmental, and antimicrobial applications.


Assuntos
Inibidores de beta-Lactamases , beta-Lactamases , Humanos , Inibidores de beta-Lactamases/farmacologia , Antibacterianos/farmacologia , Monobactamas , Penicilinas
2.
Sci Data ; 10(1): 603, 2023 09 09.
Artigo em Inglês | MEDLINE | ID: mdl-37689692

RESUMO

Mantamonads were long considered to represent an "orphan" lineage in the tree of eukaryotes, likely branching near the most frequently assumed position for the root of eukaryotes. Recent phylogenomic analyses have placed them as part of the "CRuMs" supergroup, along with collodictyonids and rigifilids. This supergroup appears to branch at the base of Amorphea, making it of special importance for understanding the deep evolutionary history of eukaryotes. However, the lack of representative species and complete genomic data associated with them has hampered the investigation of their biology and evolution. Here, we isolated and described two new species of mantamonads, Mantamonas vickermani sp. nov. and Mantamonas sphyraenae sp. nov., for each of which we generated transcriptomic sequence data, as well as a high-quality genome for the latter. The estimated size of the M. sphyraenae genome is 25 Mb; our de novo assembly appears to be highly contiguous and complete with 9,416 predicted protein-coding genes. This near-chromosome-scale genome assembly is the first described for the CRuMs supergroup.


Assuntos
Eucariotos , Genoma , Transcriptoma , Eucariotos/genética , Perfilação da Expressão Gênica , Genômica , Filogenia
3.
Sci Rep ; 10(1): 18115, 2020 10 22.
Artigo em Inglês | MEDLINE | ID: mdl-33093545

RESUMO

Calsequestrin is among the most abundant proteins in muscle sarcoplasmic reticulum and displays a high capacity but a low affinity for Ca2+ binding. In mammals, calsequestrin is encoded by two genes, CASQ1 and CASQ2, which are expressed almost exclusively in skeletal and cardiac muscles, respectively. Phylogenetic analysis indicates that calsequestrin is an ancient gene in metazoans, and that the duplication of the ancestral calsequestrin gene took place after the emergence of the lancelet. CASQ2 gene variants associated with catecholaminergic polymorphic ventricular tachycardia (CPVT) in humans are positively correlated with a high degree of evolutionary conservation across all calsequestrin homologues. The mutations are distributed in diverse locations of the calsequestrin protein and impart functional diversity but remarkably manifest in a similar phenotype in humans.


Assuntos
Cálcio/metabolismo , Calsequestrina/genética , Calsequestrina/metabolismo , Cardiopatias/patologia , Mutação , Filogenia , Sequência de Aminoácidos , Animais , Sinalização do Cálcio , Calsequestrina/química , Cardiopatias/genética , Cardiopatias/metabolismo , Humanos , Fenótipo , Conformação Proteica , Homologia de Sequência de Aminoácidos
4.
Sensors (Basel) ; 17(1)2016 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-28036081

RESUMO

This manuscript describes the design considerations, implementation, and laboratory validation of an odor sensing module whose purpose is to support people that suffer from incontinence. Because of the requirements expressed by the affected end-users the odor sensing unit is realized as a portable accessory that may be connected to any pre-existing smart device. We have opted for a low-cost, low-power consuming metal oxide based gas detection approach to highlight the viability of developing an inexpensive yet helpful odor recognition technology. The system consists of a hotplate employing, inkjet-printed p-type semiconducting layers of copper(II) oxide, and chromium titanium oxide. Both functional layers are characterized with respect to their gas-sensitive behavior towards humidity, ammonia, methylmercaptan, and dimethylsulfide and we demonstrate detection limits in the parts-per-billion range for the two latter gases. Employing a temperature variation scheme that reads out the layer's resistivity in a steady-state, we use each sensor chip as a virtual array. With this setup, we demonstrate the feasibility of detecting odors associated with incontinence.


Assuntos
Tecnologia sem Fio/instrumentação , Amônia/química , Cobre/química , Desenho de Equipamento , Odorantes/análise , Titânio/química
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