Clinostating effects on Apogee wheat resistance to wheat streak mosaic virus.

The effects of vertical and horizontal clinostating with container velocity 2 rpm and platform velocity 1 rpm on Apogee wheat resistance to wheat streak mosaic virus (WSMV) were studied. For the first time the yield of grain was obtained from both, healthy and the WSMV-infected wheat plants under conditions of simulated microgravity (clinorotation) which is accounted for by the inclusion of various mechanisms in the process of plant adaptation to the factors of stresses. For the first time the stages of viral infection development were elucidated under the conditions of prolonged clinostating and the presence of the WSMV was detected in wheat plants on the 18th day after inoculation employing the method of polymerase chain reaction (PCR). In the test variant with vertical clinostating (R=1,0) the least favorable conditions for viral reproduction were identified. In the same variant the increases of the total nitrogen content in the ears were observed which may be an evidence of interferon-like protein synthesis in plant similar to the interferon system functioning in animals.

[Effect of temperature on the infectivity of the iridovirus and densonucleosis virus of blood-sucking mosquitoes]. / Vliianie temperatury na infektsionnost' iridovirusa i virusa densonukleoza krovososushchikh komarov.

Mosquito densonucleosis virus and iridovirus were used to study the influence of temperature on their infectivity. Biological assays of mosquito densonucleosis virus were carried out in larvae of I-II instar of Aedes aegypti mosquitoes, those of mosquito iridovirus in larvae of Galleria mellonella. The rate of inactivation was found to be directly dependent on the dose of the heat treatment. Treatment at 60 degrees C led to complete loss of iridovirus infectivity; the virus activity declined considerably after treatment at 50 degrees and 40 degrees C for 60 min. Mosquito densonucleosis virus infectivity was eliminated completely at 65 degrees C. The decline of infectivity of both viruses was intermittent.

[Effect of the mosquito iridovirus on the calmodulin content of the larval tissues of the honeycomb moth]. / Vliianie iridovirusa komarov na soderzhanie kal'modulina v tkaniakh lichinok bol'shoi voshchinnoi moli.

The influence of iridovirus infection on the content of calmodulin in cells of honeycomb moth was studied, and a significant increase in the content of calmodulin in the infected cells was established which may indicate increased transcription of this protein gene.