Psychosocial mediators and moderators of a school-based physical activity intervention among Brazilian adolescents.

School-based interventions are needed due to the low levels of physical activity (PA) in adolescents. The aim is to examine the mediation effects of psychosocial factors (attitude, self-efficacy, social support from parents, friends, general teachers, and PE teachers, and environment school perception) and moderation by sex, school grade, and socioeconomic level of a school-based PA intervention on the PA practice among adolescents. The Movimente Programme is a randomised controlled trial at schools in southern Brazil (n = 921 adolescents). Strategies included teacher training, educational actions, and environmental changes. Adolescents self-reported their weekly PA. Potential psychosocial mediators and moderators were investigated through validated questionnaires in a Brazilian sample. The product of the coefficients with percentile bootstrapping 95% confidence interval was performed. The Movimente Programme was related to positive changes in adolescents' perception of the school environment and social support from general and physical education teachers. Most psychosocial variables (attitude, self-efficacy, social support from friends, and social support from teachers) were associated with PA, but none mediated the impact of the Movimente Programme on PA. Results varied according to sex and school grade. The Movimente Programme increased the adolescents' perception of the school environment and social support from teachers, but no mediators were confirmed.

The use of a biopsychosocial model in the treatment of patients with chronic.

OBJECTIVE: To explore the available knowledge on the application of biopsychosocial model (BPS) in patients with chronic low back pain (CLBP). METHODS: A scoping review, including 32 papers that used a BPS to manage adult patients with CLBP, published in any language. Six different databases were searched. RESULTS: According to the description of the protocols, most of them use BPS for assessment of the patients. In this first stage the most often evaluated were pain and disability, kinesiophobia and quality of life, and work-related factors. The intervention most used associate psychological and physical domains using pain education or cognitive functional therapy and active exercise. Even though was not the most common, in the social domain occupational, behavioral and family interventions were observed. CONCLUSION: BPS is more often used as combination of physical and psychological aspects, being the social domain restricted to work-related factors. Patien'ts perception of their health status is little explored, and it is suggested that the International Classification of Functioning, Disability and Health be used. PRACTICE IMPLICATION: Patient perception as well as social factors in addition to occupational ones should be included in the clinical approach of the patient with CLBP and should be further explored in research.

Self-rated health among adolescents from vulnerable areas and their sociodemographic, lifestyle and contextual factors: A multilevel analysis.

PURPOSE: Understanding self-rated health in young people can help orient global health actions, especially in regions of social vulnerability. The present study analysed individual and contextual factors associated with self-rated health in a sample of Brazilian adolescents. DESIGN AND METHODS: Cross-sectional data from 1272 adolescents (aged 11-17; 48.5% of girls) in low human development index (HDI) neighbourhoods were analysed (HDI from 0.170 to 0.491). The outcome variable was self-rated health. Independent variables relating to individual factors (biological sex, age and economic class) and lifestyle (physical activity, alcohol, tobacco consumption and nutritional state) were measured using standardised instruments. The socio-environmental variables were measured using neighbourhood registered data where the adolescents studied. Multilevel regression was used to estimate the regression coefficients and their 95% confidence intervals (CI). RESULTS: Good self-rated health prevalence was of 72.2%. Being male (B: -0.165; CI: -0.250 to -0.081), age (B: -0.040; CI: -0.073 to -0.007), weekly duration of moderate to vigorous physical activity (B: 0.074; CI: 0.048-0.099), body mass index (B: -0.025; CI: -0.036 to -0.015), number of family healthcare teams in the neighbourhood (B: 0.019; CI: 0.006-0.033) and dengue incidence (B: -0.001; CI: -0.002; -0.000) were factors associated with self-rated health among students from vulnerable areas. CONCLUSIONS/PRACTICAL IMPLICATIONS: Approximately three in every 10 adolescents in areas of social vulnerability presented poor self-rated health. This fact was associated with biological sex and age (individual factors), physical activity levels and BMI (lifestyle) and the number of family healthcare teams in the neighbourhood (contextual).

Physical Education for Health Among School-Aged Children and Adolescents: A Scoping Review of Reviews.

BACKGROUND: Physical education (PE) classes in schools are considered relevant to implement interventions, especially focused on physical activity. However, evidence overviews on how PE classes contribute to general health (physical, social, affective, and cognitive domains) are still needed. Thus, we summarized evidence synthesis (eg, systematic reviews) that addressed the contribution of PE classes to the health of school-aged children and adolescents. METHODS: We performed a scoping review with searches in 8 databases and institutional websites to find systematic reviews or meta-analyses that answered this review's research question. Data charting form included the identification of the study, health outcomes, and PE classes' strategies (policies and environment, curriculum, appropriate instructions, and evaluation). An interactive process was performed to build the evidence summary. RESULTS: An initial search yielded 2264 titles, and 49 systematic reviews (including 11 with meta-analysis) were included in this review. Most documents reported the main benefits of PE classes on physical domain outcomes (eg, physical activity, cardiorespiratory fitness, body mass index, and fundamental motor skills). However, evidence on the benefits of PE classes in affective (eg, enjoyment, motivation, and autonomy); social (eg, cooperation, problem-solving, and making friends); and cognitive (eg, memory, attention, concentration, and decision making) domains were found. Strategies on PE classes for health benefits were highlighted. CONCLUSIONS: These elements were detailed in the evidence summary, which may be considered to guide researchers, teachers, and practitioners to define research and practice priorities on PE class interventions for health in the school context.

National policies on physical activity from 64 countries with different economies: a scoping review with thematic analysis.

In 2018, the World Health Organization (WHO) launched a Global Action Plan on Physical Activity (PA), which included 20 policy actions for creating active societies, environments, people and systems. The objective of this scoping review was to summarize the themes/contents of national PA policies/plans conforming to the WHO's proposals and the country's economy. This review followed Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines for scoping reviews. A systematic search of electronic databases (Web of Science, Medline/PubMed, LILACS, PsycINFO, Scopus and SPORTDiscus) and 441 government documents/websites from 215 countries/territories was conducted (February 2021). Documents on national-level policies, published in English, Spanish and Portuguese since 2000, were eligible. The information on content and structure was systematically extracted and summarized into dimensions proposed by the WHO: active societies, environments, people and systems. The search identified 888 article references and 586 potentially relevant documents. After the screening, 84 policy documents from 64 countries were eligible. Most documents (n = 46) provided detailed PA policies/plans amid other health topics (e.g. non-communicable diseases, named 'general documents'), and 38 were PA-specific. The content analysis merged 54 visions, 65 missions, 108 principles, 119 objectives, 53 priorities, 105 targets, 126 indicators and 1780 actions/strategies from 38 PA-specific and 46 general documents. Among the PA-specific documents, the active system's dimension was the most contemplated in the principles (n = 43), priorities (n = 51) and action/strategies (n = 530) elements. At the same time, the objectives (n = 39), targets (n = 52) and indicators (n = 58) presented contents more frequently related to the active people dimension. For the general documents, all principles (n = 4), objectives (n = 14) and priorities (n = 7) were related to the dimension of active people, while target (n = 51), indicator (n = 53) and actions/strategies (n = 292) elements presented content related to all dimensions. The increase in countries with national PA policies/plans should be followed by improving the current ones because important dimensions seem to be not considered in these documents. This will facilitate a global PA agenda that considers the complexity and multidimensionality of PA promotion.

Inter-Examiner and Intra-Examiner Reliability of Quantitative and Qualitative Ultrasonography Assessment of Peripheral and Respiratory Muscles in Critically Ill Patients.

ICU patients are exposed to several factors that can lead to muscle structural and functional changes, and ultrasonography can identify them. Although several studies have analyzed the reliability of muscle ultrasonography assessment, a protocol with more muscle assessments becomes a challenge. The aim of this study was to analyze the inter and intra-examiner reliability of peripheral and respiratory muscle ultrasonography assessment in critically ill patients. The sample size was 10 individuals aged ≥ 18 years who were admitted to the ICU. Practical training of four health professionals from different backgrounds was performed. After training, each examiner acquired three images to assess the thickness and echogenicity of the muscle groups: biceps brachii, forearm flexor group, quadriceps femoris, tibialis anterior and diaphragm. For the reliability analysis, an intraclass correlation coefficient was performed. Six hundred US images were analyzed for muscle thickness and 150 for echogenicity. Excellent intra-examiner reliability for echogenicity (ICC: 0.867-0.973) and inter-examiner reliability for thickness were found in all muscle groups (ICC: 0.778-0.942). For muscle thickness intra-examiner reliability, excellent results were found (ICC: 0.798-0.988), with a "good" correlation in one diaphragm assessment (ICC: 0.718). Excellent inter- and intra-examiner reliability of the thickness assessment and intra-examiner echogenicity of all muscles analyzed were found.

Physical activity, sedentary behavior and sleep measured by smartwatch: a scoping review protocol / Atividade física, comportamento sedentário e sono medidos por smartwatch: protocolo de revisão de escopo

The health impacts of physical activity (PA), sedentary behavior (SB), and sleep are well established; thereby, the identification of instruments to assess and monitor these behaviors at a populational lev-el is relevant. In this context, smartwatches, which are wristwatch-shaped devices equipped with sen-sors, have been identified as alternatives for objectively measuring PA, SB, and sleep. Therefore, this protocol aimed at describing the goals and methods of a scoping review to map the literature on the use of smartwatches to objectively measure PA, SB, and/or sleep across the lifespan (e.g., children, adolescents, adults, and elderly) and in different contexts. Studies will be included if they use smart-watches to objectively measure at least one of the behaviors (PA, SB, and sleep) in children, adoles-cents, adults, and older adults, published after 2013. No language filter will be applied. Searches will be carried out in six databases (Pubmed/Medline, Scopus, Web of Science, LILACS, IEEE Xplore Digital Library, Scielo, Health Technology Assessment Database, and Cochrane clinical trials) and two clinical trial repositories. The screening and data extraction will be performed independently by two authors who had previous experience in reviews and technologies. The synthesis of the results will follow the Joanna Briggs Institute framework for extracting the results in scoping reviews. The results can contribute to scientific progress by identifying gaps and research trends, guiding future studies, and informing companies, healthcare professionals, and the general public who use smart-watch as a measurement tool for physical activity, sedentary behavior, and sleep

Os impactos da atividade física (AF), comportamento sedentário (CS) e sono na saúde são bem estabelecidos, tornando-se relevante identificar instrumentos que permitam avaliar e monitorar esses comportamentos em nível populacional. Nesse contexto, os smartwatches, que são dispositivos em formato de relógio de pulso, com-postos por sensores, tem sido apontado como alternativa para mensurar objetivamente AF, CS e sono. Portanto, o objetivo deste protocolo foi descrever os objetivos e métodos de uma revisão de escopo para mapear a literatura científica sobre o uso de smartwatches para medir objetivamente AF, CS e/ou sono em diferentes populações e contextos. Os estudos serão incluídos se usarem smartwatches para medir objetivamente pelo menos um dos comportamentos (AF, CS e sono) em crianças, adolescentes, adultos e idosos, publicados após 2013. Nenhum filtro de idioma será aplicado. As buscas serão realizadas em sete bases de dados (Pubmed/Medline, Scopus, Web of Science, IEEE Xplore Digital Library, Scielo, LILACS, Health Technology Assessment Database e Cochrane Clinical Trials) e dois repositórios de ensaios clínicos. A triagem e extração dos dados serão realizadas de forma independente por dois autores com experiência prévia em revisões e tecnologias. A síntese dos resultados seguirá o framework do Joanna Briggs Institute para extração dos resultados nas revisões de escopo. Os resultados podem contribuir para o progresso científico, identificando lacunas e tendências de pesquisa, orientando futuros estudos, empresas que atuam neste mercado, profissionais de saúde e o público em geral que utilizam smartwatch como um instrumento de medição para atividade física, comportamento sedentário e sono

A systematic review of the clustering and correlates of physical activity and sedentary behavior among boys and girls.

Identifying the clustering and correlates of physical activity (PA) and sedentary behavior (SB) is very important for developing appropriate lifestyle interventions for children and adolescents. This systematic review (Prospero CRD42018094826) aimed to identify PA and SB cluster patterns and their correlates among boys and girls (0-19 years). The search was carried out in five electronic databases. Cluster characteristics were extracted in accordance with authors' descriptions by two independent reviewers and a third resolved any disagreements. Seventeen studies met the inclusion criteria and the population age ranged from six to 18 years old. Nine, twelve, and ten cluster types were identified for mixed-sex samples, boys, and girls, respectively. While girls were in clusters characterized by "Low PA Low SB" and "Low PA High SB", the majority of boys were in clusters defined by "High PA High SB" and "High PA Low SB". Few associations were found between sociodemographic variables and all cluster types. Boys and girls in "High PA High SB" clusters had higher BMI and obesity in most of the tested associations. In contrast, those in the "High PA Low SB" clusters presented lower BMI, waist circumference, and overweight and obesity. Different cluster patterns of PA and SB were observed in boys and girls. However, in both sexes, a better adiposity profile was found among children and adolescents in "High PA Low SB" clusters. Our results suggest that it is not enough to increase PA to manage the adiposity correlates, it is also necessary to reduce SB in this population.

Home Based Rehabilitation in Children and Adolescents with Cystic Fibrosis: A Systematic Review with Meta-Analysis and Grade Recommendations.

OBJECTIVE: The objective of this study is to elucidate the effectiveness of home based rehabilitation (HBR) and compare its results with those obtained in conventional rehabilitation (CR) programs, carried out in clinics and/or outpatient clinics. METHODS: Searches were conducted in five databases of randomized clinical trials. Study selection, data extraction, and assessment of the methodological quality of included studies were conducted independently by two reviewers, with discrepancies resolved by a third reviewer. RESULTS: The results demonstrate post-intervention values favorable to the use of HBR when compared to control group in the outcomes of forced expiratory volume in 1 second (FEV1) (MD = 14% CI: 5.42 to 22.58, p = 0.001), forced vital capacity (FVC) (MD = 8.00% CI: 0.83 to 15.17, p = 0.03) and quality of life by the Cystic Fibrosis Questionnaire - revised in the categories (Child version score" (MD= 0.71%CI: 0.15 to 1.27, p = 0.01) and "Parent version score" (MD= 0.67%CI: 0.11 to 1.23, p = 0.02). Furthermore, we noticed an increase in the distance covered in the 6-minute walk test (MD= 34.75%CI: -8.00 to 77.50, p = 0.14), in favor of HBR. CONCLUSIONS: We found that supervised or partially supervised HBR promotes improvements in FEV1, FVC and related quality of life in children and/or adolescents with cystic fibrosis.

Comparison between exercise therapy and non-hydrolyzed collagen (UC-II) in functionality and quality of life in women with knee osteoarthritis : A randomized controlled clinical trial.

BACKGROUND: Knee osteoarthritis (OA) is characterized by a progressive degeneration of cartilage and menisci, leading to pain and locomotor disability. Here, we aimed to assess the effect of an exercise protocol and the oral use of non-hydrolyzed collagen (UC-II) on the functionality and quality of life of women with knee OA. MATERIAL AND METHODS: Individuals were divided into three groups (CG [control group]; MG [medication group]; EG [exercise group]). In the CG there was no intervention, while MG received an oral dose (1 capsule/day) of UC-II and the EG held 12 sessions of an exercise protocol. RESULTS: In the functionality tests (6-min walk test, 6MWT and timed up and go test [TUG]) the EG (p < 0.001/p = 0.020) and MG (p = 0.010/p = 0.010) revealed a significant improvement when compared to the CG. In the analysis of quality of life by WOMAC, a significant improvement was found only in the EG (p = 0.030) when compared to the CG; the same happened in the stiffness domain (EG, p = 0.010), despite in the pain domain, both the EG (p < 0.001) and the MG (p = 0.060) were better than the CG. CONCLUSION: Data obtained here reveal that an exercise protocol and UC-II have similar effects for functionality, despite exercise being superior in promoting the quality of life score.

Lack of molecular evidence of fecal-borne viruses in capybaras from São Paulo state, Brazil, 2018-2020: a minor public health issue.

Capybara (Hydrochoerus hydrochaeris) is the world's largest rodent species distributed throughout South America. These animals are incredibly tolerant to anthropogenic environments and are occupying large urban centers. Capybaras are known to carry potentially zoonotic agents, including R. rickettsia, Leishmania spp., Leptospira spp., Trypanosoma spp., Salmonella spp., Toxoplasma gondii, and rabies virus. Focusing on the importance of monitoring potential sources of emerging zoonotic viruses and new viral reservoirs, the aim of the present study was to assess the presence of fecal-borne viruses in the feces of capybaras living in urban parks in São Paulo state, Brazil. A total of 337 fecal samples were collected between 2018 and 2020 and screened for the following: (i) Rotavirus group A (RVA) by ELISA; (ii) non-RVA species and Picobirnavirus (PBV) using PAGE; (iii) Human Bocaparvovirus (HBoV), Bufavirus (BuV), Tusavirus (TuV), and Cutavirus (CuV) qPCR; (iv) Human Enterovirus (EV), Norovirus GII (NoV), and Hantavirus by in houses RT-qPCR; (v) SARS-CoV-2 via commercial RT-qPCR kit assay; and (vi) Astrovirus (AstV) and Adenovirus (AdV) using conventional nested (RT)-PCRs. All fecal samples tested were negative for fecal-borne viruses. This study adds further evidence that the fecal-borne viruses is a minor public health issue in Brazilian capybaras, at least during the surveillance period and surveyed areas. Continuous monitoring of sylvatic animals is essential to prevent and control the emergence or re-emergence of newly discovered virus as well as viruses with known zoonotic potential.

School Nursing Guide for student health promotion: construction and validity / Guía de Enfermería Escolar para la promoción de la salud de jóvenes estudiantes: construcción y validación / Guia de Enfermagem Escolar para promoção da saúde de jovens estudantes: construção e validação

ABSTRACT Objectives: to describe the process of construction and validity of a School Nursing Guide for student health promotion. Methods: a methodological study, carried out from February to December 2021, composed of Convergent Care Research based on Pender's Health Promotion Model. Based on the literature and dialogue with 11 nurses in the seven online focus groups, actions were constructed. Subsequently, 24 judges assessed content and appearance. Results: the guide proposes strategies for developing school nursing practices focusing on health promotion. The Appearance Validity Index ranged from 0.63 to 1.0, and the total was 0.84. The Content Validity Index ranged from 0.95 to 1.0, and the total was 0.997. Conclusions: the guide incorporated the needs of young people recognized by professionals, and the assessment phase confirms its validity, and can be used in the context of practice with young people.

RESUMEN Objetivos: describir el proceso de construcción y validación de la Guía Escolar de Enfermería para la promoción de la salud de los jóvenes estudiantes. Métodos: estudio metodológico, realizado de febrero a diciembre de 2021, compuesto por Investigación de Atención Convergente basada en el Modelo de Promoción de la Salud de Pender. Con base en la literatura y el diálogo con 11 enfermeras en los siete grupos focales en línea, se construyeron acciones. Posteriormente, 24 jueces evaluaron el contenido y la apariencia. Resultados: la guía propone estrategias para el desarrollo de prácticas escolares de enfermería con foco en la promoción de la salud. El índice de validez de apariencia osciló entre 0,63 y 1,0 y el total fue de 0,84. El índice de validez de contenido osciló entre 0,95 y 1,0 y el total fue de 0,997. Conclusiones: la guía incorporó las necesidades de los jóvenes reconocidas por los profesionales, y la fase de evaluación confirma su validación, pudiendo ser utilizada en el contexto de la práctica con jóvenes.

RESUMO Objetivos: descrever o processo de construção e validação do Guia de Enfermagem Escolar para promoção da saúde de jovens estudantes. Métodos: estudo metodológico, realizado de fevereiro a dezembro de 2021, composto por Pesquisa Convergente-Assistencial fundamentada no Modelo de Promoção da Saúde de Pender. A partir da literatura e diálogo com 11 enfermeiros nos sete grupos focais online, foram construídas ações. Posteriormente, 24 juízes avaliaram o conteúdo e a aparência. Resultados: o guia propõe estratégias para o desenvolvimento das práticas de enfermagem escolar enfocando a promoção da saúde. O Índice de Validade de Aparência variou de 0,63 a 1,0, e o total foi de 0,84. O Índice de Validade de Conteúdo variou de 0,95 a 1,0, e o total foi de 0,997. Conclusões: o guia incorporou as necessidades dos jovens reconhecidas pelos profissionais, e a fase de avaliação confirma sua validação, podendo ser utilizado no contexto da prática com jovens.

Desigualdades de cor/raça na disponibilidade de instalações para a prática de atividades físicas e esportes nas escolas públicas de educação básica do Brasil / Color/race inequalities in the availability of facilities for physical activities and sports in public elementary schools in Brazil / Desigualdades de color/raza en la disponibilidad de instalaciones para la práctica de actividades físicas y deportivas en escuelas públicas de educación básica en Brasil

RESUMO O objetivo deste estudo ecológico foi estimar desigualdades nas instalações para atividades físicas e esportes (AF) nas escolas públicas de Educação Básica brasileiras (n=70.276), conforme a proporção de matrículas de estudantes autodeclarados negros. Os resultados mostraram que escolas urbanas e rurais com maior proporção de matrículas de estudantes negros (≥60%) apresentaram menos pátio descoberto, quadra coberta e descoberta, parque infantil, estúdio de dança e materiais para a prática desportiva e recreação. Essas desigualdades são traços do racismo, que é estruturante na sociedade brasileira e é institucionalizado na oferta de condições desfavoráveis ao engajamento em AF nas escolas públicas que atendem um maior número de alunos pretos e pardos.

ABSTRACT The aim of this study was to estimate inequality in physical activity and sports facilities at Brazilian public elementary schools, according to percent of self-reported black students. Our results showed that schools from urban or rural areas with a higher percentage of enrollments of self-reported black students (≥60%) presented fewer outdoor courtyards, sport court indoor and outdoor, playgrounds, dance studios, and materials for sports and recreation. These inequalities are a feature of racism, which is structural in Brazilian society and institutionalized in unfavorable conditions for engaging in physical activity and sports at public elementary schools where there is a higher number of black students.

RESUMEN Este estudio ecológico tuvo como objetivo estimar las desigualdades en las instalaciones para actividades físicas y deportivas (AF) en las escuelas públicas brasileñas de Educación Básica (n=70.276), según la proporción de matrículas de alumnos que se declararon negros. Los resultados mostraron que las escuelas urbanas y rurales con mayor proporción de matrículas de estudiantes negros (≥60%) tenían menos patio al aire libre, canchas interiores y exteriores, parques infantiles, estudios de danza y materiales para deportes y recreación. Esas desigualdades son rasgos del racismo, que es estructural en la sociedad brasileña y está institucionalizado al ofrecer condiciones desfavorables para la práctica de AF en las escuelas públicas que atienden a un mayor número de alumnos negros y pardos.

Terapia genética de injeção intravítrea de rAAV2-ND4 para neuropatia óptica hereditária de Leber: uma revisão sistemática / rAAV2-ND4 intravitreal injection gene therapy for Leber's hereditary optic neuropathy: a systematic review

RESUMO A neuropatia óptica hereditária de Leber é uma doença mitocondrial hereditária neurodegenerativa. A taxa potencial de recuperação espontânea é controversa na literatura. A terapia genética tem sido estudada como suporte aos pacientes. O objetivo desta revisão foi avaliar qualitativamente a segurança, os efeitos adversos e a eficácia da terapia gênica disponível. Trata-se de uma revisão sistemática de artigos indexados nas bases de dados PubMed®, Biblioteca Virtual em Saúde, SciELO, Cochrane, ScienceDirect, Scopus e Lilacs no primeiro semestre de 2021. Os critérios de inclusão e filtros foram: artigos relacionados ao tema, estudos randomizados, ensaios clínicos, trabalhos em humanos, últimos 5 anos, nas línguas portuguesa, inglesa e espanhola e texto completo disponível gratuitamente. Os parâmetros de exclusão foram: artigos duplicados, fuga ao tema, artigos de revisão, trabalhos não disponíveis e que fugiam aos critérios de inclusão. O coeficiente de kappa foi 0,812. A terapia não apresentou efeitos adversos sérios em nenhum dos artigos selecionados, e os efeitos menores sofreram 100% de remissão espontânea após o tratamento. Apesar de NAbs terem sido encontrados no soro de alguns pacientes, não houve associação entre a resposta imune adaptativa e a injeção do vetor viral. O tratamento foi eficaz na melhora da acuidade e campo visual. Vários estudos confirmaram a eficácia da terapia gênica, em doses baixas e médias, na melhora da acuidade visual e também sobre os efeitos adversos comuns relacionados à altas doses. A resposta imune humoral e a variação dos NAbs no soro foi citada em mais de um artigo. A terapia foi eficaz na melhora da acuidade visual e os efeitos adversos que surgiram foram tratados facilmente. No entanto, a resposta imune humoral ainda precisa ser estudada.

ABSTRACT Leber's Hereditary Optic Neuropathy (LHON) is an inherited neurodegenerative mitochondrial disease. The potential rate of spontaneous recovery is controversial in the literature. Gene therapy has been studied to support patients. The objective of this review was to qualitatively assess the safety, adverse effects, and efficacy of available gene therapy. This is a systematic review of articles indexed in PubMed®, VHL, SciELO, Cochrane, ScienceDirect, Scopus, and Lilacs databases, in the first half of 2021. Inclusion criteria and filters were: articles related to the topic, randomized studies, clinical trials, work in humans, last 5 years, in Portuguese, English, and Spanish and full text available for free. The exclusion parameters were: duplicate articles, not related to the topic, review articles, not available works, and works that did not meet the inclusion criteria. The kappa coefficient was 0.812. The therapy had no serious adverse effects in any of the selected articles, and minor effects experienced 100% spontaneous remission after treatment. Although NAbs were found in the serum of some patients, there was no association between the adaptive immune response and the injection of the viral vector. The treatment was effective in improving acuity and visual field. Several studies have confirmed the effectiveness of gene therapy, at low and medium doses, in improving visual acuity and also on common adverse effects related to high doses. The humoral immune response and the variation in serum NAbs was cited in more than one article. The therapy was effective in improving visual acuity and the adverse effects that arose were easily treated. However, the humoral immune response still needs to be studied.

School Nursing Guide for student health promotion: construction and validity.

OBJECTIVES: to describe the process of construction and validity of a School Nursing Guide for student health promotion. METHODS: a methodological study, carried out from February to December 2021, composed of Convergent Care Research based on Pender's Health Promotion Model. Based on the literature and dialogue with 11 nurses in the seven online focus groups, actions were constructed. Subsequently, 24 judges assessed content and appearance. RESULTS: the guide proposes strategies for developing school nursing practices focusing on health promotion. The Appearance Validity Index ranged from 0.63 to 1.0, and the total was 0.84. The Content Validity Index ranged from 0.95 to 1.0, and the total was 0.997. CONCLUSIONS: the guide incorporated the needs of young people recognized by professionals, and the assessment phase confirms its validity, and can be used in the context of practice with young people.

Is oral microbiota associated with overweight and obesity in children and adolescents? A systematic review.

This article aims to verify the relationship between the composition and diversity of oral microbiota with overweight and obese children and adolescents. This systematic review was registered in PROSPERO, followed PRISMA 2020, and included an electronic search until March 2022, in PubMed/MEDLINE, Web of Science, Scopus, and The Cochrane Library databases. Studies were eligible if they compared the oral microbiota according to nutrition status among children and adolescents. Independent peers using JBI Critical Appraisal Checklists assessed the quality of studies. Eleven studies were eligible to be included in this review, with a total of 1,695 children and adolescents, 224 were obese, 190 were overweight, 1,154 were eutrophics and 127 were underweight. The most frequent phyla in overweight and obese children and adolescents, in comparison to their counterparts were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria. It was identified that nine of the eleven articles selected showed an association between oral microbiota and overweight and obesity in children and adolescents. We observed that there is an important association between oral bacterial composition diversity and overweight and obesity. This finding indicates the relevance of the evaluation and surveillance in oral health to control cases of overweight and obesity in children and adolescents.

Uso de redes neurais para a predição de diagnóstico de AVE: uma revisão sistemática / Use of neural networks for stroke diagnosis prediction: a systematic review neural networks and stroke prediction

Fundamentos: O Acidente Vascular Encefálico (AVE) é uma síndrome de déficit neurológico agudo atribuído à lesão vascular do Sistema Nervoso (SN). As técnicas de Inteligência Artificial (IA) na Medicina ­ como algoritmos de Redes Neurais Artificiais (RNAs) ­ têm ajudado na tomada de decisões clínicas voltadas para essa condição. Objetivo: o objetivo desta revisão será avaliar como as redes neurais artificiais estão sendo utilizadas para a predição de diagnóstico de AVE. Métodos: Trata-se de uma revisão sistemática de artigos indexados nas bases de dados PubMed, BVS, SciELO, Cochrane e SpringerLink, entre janeiro e fevereiro de 2022. Os critérios de inclusão e filtros para esse trabalho foram: artigos relacionados ao tema, estudos randomizados, coorte e ensaios clínicos, trabalhos em humanos, realizados nos últimos 5 anos, apenas nos idiomas Português, Inglês e Espanhol e com texto completo disponível gratuitamente. Os parâmetros de exclusão foram: artigos duplicados, fuga ao tema, artigos de revisão e trabalhos que não preenchiam todos os critérios de inclusão. Resultados: As RNAs estão sendo utilizadas, principalmente, para avaliação de áreas de lesões isquêmicas e hemorrágicas por métodos de segmentação e os exames mais utilizados para a modelagem dos programas têm sido Ressonância Magnética (RM) e Tomografia Computadorizada (TC). Além da TC e RM, a angiorressonância e angiotomografia também estão sendo utilizadas para o modelamento do algoritmo e são úteis por apresentarem maior sensibilidade para detecção de infartos. Conclusão: Algoritmos de segmentação e classificação aplicados nas RNAs fazem parte da medicina personalizada e servem de base para médicos na prática clínica.

Background: Stroke is an acute neurological deficit syndrome attributed to vascular injury to the Nervous System (NS). Artificial Intelligence (AI) techniques in Medicine ­ such as Artificial Neural Networks (ANNs) algorithms ­ have helped in making clinical decisions aimed at this condition. Objective: the objective of this review will be to evaluate how artificial neural networks are being used to predict the diagnosis of stroke. Methods: This is a systematic review of articles indexed in PubMed, VHL, SciELO, Cochrane and SpringerLink databases, between January and February 2022. The inclusion criteria and filters for this work were: articles related to the topic, studies randomized, cohort and clinical trials, studies in humans, carried out in the last 5 years, only in Portuguese, English and Spanish and with full text available free of charge. The exclusion parameters were: duplicate articles, escape from the topic, review articles and works that did not meet all the inclusion criteria. Results: ANNs are being used mainly for the evaluation of areas of ischemic and hemorrhagic lesions by segmentation methods and the most used exams for modeling the programs have been Magnetic Resonance Imaging (MRI) and Computed Tomography (CT). In addition to CT and MRI, magnetic resonance angiography and tomography angiography are also being used to model the algorithm and are useful because they have greater sensitivity for detecting infarctions. Conclusion: Segmentation and classification algorithms applied in ANNs are part of personalized medicine and serve as a basis for physicians in clinical practice.

Is Participation in Physical Education Classes Related to Physical Activity and Sedentary Behavior? A Systematic Review.

BACKGROUND: The aim of this systematic review was to summarize the results and assess the methodological quality of studies that analyzed the relation between physical education participation, physical activity, and sedentary behavior in schoolchildren. METHODS: Searches were conducted for original cross-sectional and longitudinal observational studies published in Portuguese, English, and Spanish between January 2007 and August 2020, on the PubMed, Web of Science, Scientific Electronic Library Online, Education Resources Information Center, and Scopus databases. RESULTS: A total of 60 articles (68 independent samples) were included in the revision (58 cross-sectional and 2 longitudinal observational studies). With regard to methodological quality, 27%, 52%, and 21% of the studies were classified as high, moderate, and low methodological quality, respectively. Physical activity was analyzed in 93% of the studies (n = 56) and sedentary behavior in 33% (n = 20). The higher frequency of physical education participation was associated with higher physical activity levels (56 of 68 results - 54/65 cross-sectional and 2/3 longitudinal studies) and less sedentary behavior (14 of 24 results), even after stratifying analyses by type and methodological quality. CONCLUSION: Physical education class participation may contribute to students being physically more active and less likely to engage in sedentary behavior.

Results from Brazil's 2022 Report Card on Physical Activity for Children and Adolescents.

This research aims to summarize the process and results of the 2022 Report Card on Physical Activity for Brazilian children and adolescents. A group of experts led by 10 PhD researchers gathered the best possible evidence on physical activity indicators. The Report Card Brazil 2022 included the top 10 indicators of physical activity and sleep, obesity, and poor mental health variables, which made up four dimensions: (I) Daily Behaviors; (II) Settings and Sources of Influence; (III) Government Strategies and Investments; and (IV) Health Outcomes. Comprehensive searches, including peer-reviewed and gray literature searches, were performed for each indicator. Data were considered from systematic reviews, local and national surveys, websites, and official information from the Brazilian Federal Government. Grades from the indicators ranged from F (Active Play) to B (School). In addition, the results found for each indicator were Overall Physical Activity (D), Organized Sport Participation (C-), Active Transportation (C), Sedentary Behaviors (D), Sleep (C), Family and Peers (C-), Community and Environment (C), Government (D+), Physical Fitness (D+), Obesity (11.7%), and Poor Mental Health (37.8%). Successfully strategies for increasing physical activity among Brazilian children and adolescents should look at the different indicators presented in this report.

Malignancy Diseases in Kidney Transplantation, Clinical Outcomes, Patient, and Allograft Survival: A Case-Control Study.

BACKGROUND: Malignancy is a well-known complication in patients after kidney transplantation (KT), but its effect on posttransplant outcomes, allograft, and patient survival remains unexplored. The aim of this study is to report the impact of the comorbidity on clinical outcome, function, and failure of an allograft kidney. METHODS: This case-control study included 101 KT patients. Twenty-six patients who developed cancer (CA) were assigned to the case group and 75 to the control group. Statistical analysis was performed using logistic regression models, and graft survival was analyzed using the Kaplan-Meier curve. RESULTS: Non-melanoma skin CA was the most common malignancy, accounting for almost 60% of cases, followed by stomach CA, prostate CA, and lymphoproliferative diseases (7.70% each). Difference in graft and patient survival was not significant between the two groups (P > .05). A tumor in nonfunctioning in the first nonfunctioning KT was identified in 1 KT patient with a second allograft and by anatomopathological was detect Fuhrman grade II renal cell carcinoma. This KT patient was in good clinical condition with serum creatinine level of 1.5 mg/dL. CONCLUSIONS: No association was observed between CA development and risk factors, including family history and smoking habit, and no differences in allograft and patient survival were found. Nevertheless, in our data, CA in KT patients occurred early after transplantation. Renal cell carcinoma in allograft failure was identified in a patient; that suggested that nephrectomy of kidney failure must be performed to avoid patient allosensitization and neoplasia. Thus, we suggest continuous screening of malignancy diseases for KT patients.