New technologies in molecular allergodiagnostics.

The article presents the characteristics of the ALEX2 (MacroArrayDX, Wien, Austria). It is designed for simultaneous detection of IgE total and specific IgE-aB to 120 extracts and 180 molecules by solid-phase enzyme immunoassay. Extracts and allergen molecules combined with nano-particles are sorbed on a solid-phase substrate, forming a macroscopic multiplex matrix - the immune allergy chip. The Institute of Clinical and Laboratory Standards (CLSI) conducted research on the verification and validation of the ALEX2 in relation to the ImmunoCAP macroarray test system (ThermoFisher Scientific, Uppsala, Sweden), which is often used in allergodiagnostics. The results obtained on the two test systems were comparable. One of the most important features of the ALEX2 test system is that unique allergen molecules and allergenic extracts are included in its composition, and a method has been found to inhibit cross-reactive hydrocarbon determinants (CCDs), which cause frequent non-specific binding of IgE-aT. The use of this test system makes it possible to carry out component allergy diagnostics with the determine of the dominant sensitizing factor in cases of mono- and polyvalent sensitization. The test results affect the determination of indications and the effectiveness of ASIT, allow assessing the risk of anaphylaxis and predicting further treatment tactics for the patient.

[Morphometric features of the temporomandibular joints according to MR studies in adult patients with dislocations of the articular discs and malocclusion of the anterior teeth]. / Morfometricheskie osobennosti visochno-nizhnechelyustnykh sustavov po dannym magnitno-rezonansnykh issledovanii u vzroslykh patsientov s dislokatsiyami sustavnykh diskov i narusheniyami okklyuzii frontal'nykh zubov.

THE PURPOSE OF THE STUDY: To study the morphological features of the temporomandibular joints (TMJ) according to MR studies in adult patients with impaired occlusion of the anterior teeth and dislocations of the articular discs. MATERIAL AND METHODS: The clinical study included 51 patients aged 17 to 47 years (mean age 28.12±7.44 years): 14 men and 37 women. On the basis of a clinical examination and MR findings of TMJ in 30 patients (mean age 26.77±7.48 years; 8 men and 22 women), TMJ dysfunction in the form of dislocation of articular discs was confirmed. In 21 patients (mean age 30.05±7.12; 15 women, 6 men), TMJ dysfunction without dislocation of articular discs was confirmed. The patients measured the value of vertical incisive overlap (overbite). Analyzing data from MR studies of TMJ, we measured the following values of both TMJs of each patient: the depth and transverse size of the articular fossa, the height of the articular tubercles and the transverse size of the head of the lower jaw. RESULTS: The examined patients with joint dislocations of TMJ had morphological features: statistically significantly deeper and narrower articular fossae, higher articular tubercles and narrower in the sagittal plane of the head of the lower jaw compared to TMJ of patients with dysfunction without dislocation of articular disks. In addition, in patients with articular disk dislocations, a statistically significant (<0.05) increase in vertical incisal overlap was noted. CONCLUSION: .TMJ in patients with dysfunction in the form of dislocations of articular discs had morphological features that allowed them to be classified as type 3.