Evaluation of NSE and S100B in patients with tick-borne encephalitis.

INTRODUCTION: The aim of this study was the assessment of neuron-specific enolase (NSE) and S-100 concentration in serum and cerebrospinal fluid (CSF) in patients with different clinical forms of tick-borne encephalitis (TBE). MATERIAL AND METHODS: The serum and CFS concentrations of S100B and NSE of 43 patients with TBE were measured with ELISA method using commercial kits: NSE and S100B Elisa Kit (DRG, Germany). Subjects were divided into: Group I-patients with meningoencephalitis (n = 17) and Group II-patients with meningitis (n = 26). None of the patients reported any neurodegenerative disorder that could affect the results of the study. The control group (CG) consisted of 13 patients. These patients were admitted to the hospital because of headache, and the CSF examination excluded inflammatory process. Samples were collected on admission (sample 1) and after treatment (sample 2). RESULTS: Neuron-specific enolase concentration in CSF was higher in group I than in group II (p = 0.0002) and controls (p = 0.04). NSE concentration was higher in the second serum and CSF sample in both groups. S100B concentration did not differ between TBE patients and controls. NSE concentration in serum after 14 days was higher in the sequelae group (34.3 ± 9.7 vs. 16.7 ± 15, p = 0.04). Also, NSE serum sample 2/serum sample 1 ratio was significantly higher in the sequelae group (3.57 ± 0.92 vs. 1.53 ± 1.99, p = 0.04). Receiver Operating Characteristic curve analysis indicated that NSE concentration in serum II differentiates sequelae group from other meningoencephalitis patients (p = 0.0001). S100B serum sample 2/CSF sample 2 ratio was lower in the sequelae group (0.05 ± 0.1 vs. 0.37 ± 0.28, p = 0.02). CONCLUSIONS: (a) Neurodegeneration process is present in TBE encephalitis. (b) NSE concentration correlates with inflammatory parameters in CSF in TBE. (c) Neurodegeneration is present even after clinical recovery of TBE. (d) NSE could be used in the prediction of TBE course. (e) S-100 did not differ between TBE patients and controls.

[Occurrence of erythema migrans in Hajnowka district in years 2004-2009]. / Wystepowanie rumienia wedrujacego w powiecie hajnowskim w latach 2004-2009.

The aim of the study was the analysis of epidemiology and clinical picture of early Lyme disease-erythema migrans (EM). There was analysed the medical documentation of 307 (126 men and 181 women) patients, who came to Outpatient Clinic of Infectious Diseases with EM in Hajn6wka in years 2004-2009. Most people (54%) lived in Hajnówka district. 51,8% of patients remembered the contact with a tick. The highest incidence of EM cases was observed in July. The average size of erythema was 10,5 cm2. In 59 cases skin lesion was accompanied by such symptoms as: itching, pain and inflammatory infiltration in the skin lesion, pain in muscles and joints, headaches, fever and cold syndroms. The most common localisation of EM were lower limbs (135 people), trunk (68) and upper limbs (29). Time of patients' visit in our Outpatient Clinic from EM notice lasted about 2 months. 6 people from analysed group were reinfected. There was used the month treatment by doxycycline in prevailing cases.