RESUMO
The effect of triton X-100 and glutaraldehyde on the changes in Ca(2+)- and Mg(2+)-ATPase activity of erythrocyte plasma membrane under irradiation of rat erythrocytes has been studied. It was found that irradiation of erythrocyte ghosts in a dose of 1000 Gy leads to the inhibition of the both enzymes. Triton X-100 enhanced the radiation effect, while glutaraldehyde decreased Ca(2+)-ATPase sensitivity to the action of radiation. The mechanisms of the effects observed are discussed.
Assuntos
ATPase de Ca(2+) e Mg(2+)/efeitos dos fármacos , ATPases Transportadoras de Cálcio/efeitos dos fármacos , Membrana Eritrocítica/efeitos dos fármacos , Glutaral/farmacologia , Octoxinol/farmacologia , Animais , ATPase de Ca(2+) e Mg(2+)/sangue , ATPases Transportadoras de Cálcio/sangue , Detergentes , Membrana Eritrocítica/enzimologia , Membrana Eritrocítica/efeitos da radiação , Membrana Eritrocítica/ultraestrutura , RatosRESUMO
Effect of electrons with the energy 5MeV on the erythrocyte ghosts structure has been investigated using fluorescent probes 1.8-ANS and DSP-12. The number of ANS binding sites was shown to decrease as affected by irradiation. It was found that radiation caused reduction of the erythrocyte membrane surface area.
Assuntos
Naftalenossulfonato de Anilina/metabolismo , Elétrons , Membrana Eritrocítica/efeitos da radiação , Corantes Fluorescentes/metabolismo , Compostos de Piridínio/metabolismo , Animais , Humanos , Radiação Ionizante , Ratos , Propriedades de SuperfícieRESUMO
Ca2+, Mg(2+)-ATP-ase of plasmatic membranes of thymocites were studied as affected by Mg ions. A complex character of the reaction rate dependence on Mg2+ concentration in the range of 0.002-25. OmM was revealed. The data obtained indicate the existence of Mg(2+)-binding centre of ATP-ase which regulates the enzyme catalytic activity.
Assuntos
ATPase de Ca(2+) e Mg(2+)/efeitos dos fármacos , ATPases Transportadoras de Cálcio/efeitos dos fármacos , Magnésio/farmacologia , Timo/efeitos dos fármacos , Animais , Catálise , Bovinos , Membrana Celular/efeitos dos fármacos , Cinética , Timo/citologiaRESUMO
Effects of non-enzymic peroxidation of lipids of thymocytes plasmic membranes on the activity of Ca(2+)-ATPase was studied. Calculations were made of kinetic and thermodynamic parameters of enzymic reaction and changes of the membrane potential during peroxidation of lipids.
Assuntos
ATPases Transportadoras de Cálcio/metabolismo , Peroxidação de Lipídeos , Animais , Bovinos , Membrana Celular/enzimologia , Membrana Celular/metabolismo , Membrana Celular/fisiologia , Cinética , Malondialdeído/metabolismo , Potenciais da MembranaRESUMO
It has been established that sorption of bromthymol blue by membranes is due to interaction with integral proteins. The value of the bond is ascribed both to the structural state and electrochemical property of membranes.
