Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Mar Pollut Bull ; 113(1-2): 380-386, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-27751574

RESUMO

To investigate the long-term weathering of oil from the Deepwater Horizon (DWH) incident, oil-soaked sand patties were collected from Gulf of Mexico beaches from Florida to Alabama over a three-year period from 2012 to 2014. Analysis of oil residues by gas chromatography with flame ionization detection (GC-FID), thin-layer chromatography with flame ionization detection (TLC-FID), and Fourier transform infrared spectroscopy (FT-IR) indicated uniformity in their chemical composition. Some variability within and between samples was observed, arising from differences in exposure to light and water, which increase the amount of weathering. Oxygenated hydrocarbons (OxHC) produced by weathering processes dominate the majority of oil residues. These OxHC have continued recalcitrance in the environment, and increase in relative abundance over time. Analyses of the bulk characteristics of oil residues via TLC-FID and FT-IR should be continued as these techniques provide important insight into the weathering state of oil residues.


Assuntos
Monitoramento Ambiental/métodos , Óleos Combustíveis/análise , Poluição por Petróleo/análise , Poluentes Químicos da Água/análise , Alabama , Biodegradação Ambiental , Cromatografia em Camada Fina , Florida , Golfo do México , Hidrocarbonetos/análise , Hidrocarbonetos/química , México , Oxirredução , Espectroscopia de Infravermelho com Transformada de Fourier , Poluentes Químicos da Água/química , Tempo (Meteorologia)
2.
Fungal Genet Biol ; 89: 18-28, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26808821

RESUMO

Microorganisms produce a wide range of natural products (NPs) with clinically and agriculturally relevant biological activities. In bacteria and fungi, genes encoding successive steps in a biosynthetic pathway tend to be clustered on the chromosome as biosynthetic gene clusters (BGCs). Historically, "activity-guided" approaches to NP discovery have focused on bioactivity screening of NPs produced by culturable microbes. In contrast, recent "genome mining" approaches first identify candidate BGCs, express these biosynthetic genes using synthetic biology methods, and finally test for the production of NPs. Fungal genome mining efforts and the exploration of novel sequence and NP space are limited, however, by the lack of a comprehensive catalog of BGCs encoding experimentally-validated products. In this study, we generated a comprehensive reference set of fungal NPs whose biosynthetic gene clusters are described in the published literature. To generate this dataset, we first identified NCBI records that included both a peer-reviewed article and an associated nucleotide record. We filtered these records by text and homology criteria to identify putative NP-related articles and BGCs. Next, we manually curated the resulting articles, chemical structures, and protein sequences. The resulting catalog contains 197 unique NP compounds covering several major classes of fungal NPs, including polyketides, non-ribosomal peptides, terpenoids, and alkaloids. The distribution of articles published per compound shows a bias toward the study of certain popular compounds, such as the aflatoxins. Phylogenetic analysis of biosynthetic genes suggests that much chemical and enzymatic diversity remains to be discovered in fungi. Our catalog was incorporated into the recently launched Minimum Information about Biosynthetic Gene cluster (MIBiG) repository to create the largest known set of fungal BGCs and associated NPs, a resource that we anticipate will guide future genome mining and synthetic biology efforts toward discovering novel fungal enzymes and metabolites.


Assuntos
Produtos Biológicos , Vias Biossintéticas/genética , Genes Fúngicos , Genoma Fúngico , Família Multigênica , Alcaloides , Sequência de Aminoácidos , Biologia Computacional , Curadoria de Dados , Fungos/genética , Filogenia , Policetídeos , Terpenos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...