RESUMO
Combinations of Virazole plus arabinofuranosylhypoxanthine (ara-Hx) and Virazole plus arabinofuranosyladenine (ara-A) were investigated in KB or BHK cells infected with types 1 or 2 herpes viruses. Combinations of Virazole and ara-Hx exhibited significant synergy as evaluated graphically (isobolograms) or by fractional inhibitory concentration (FIC) indices. Optimal ratios for the combination were 1:1 to 1:10 for Virazole to ara-Hx. At these ratios, FIC indices in the range of 0.5-0.2 were commonly observed. Combinations of Virazole and ara-A were antagonistic when observed in the presence of pentostatin, an adenosine deaminase inhibitor. In the absence of pentostatin, the minimum inhibitory concentration (MIC) of ara-A and degree of synergy with Virazole were variable.
Assuntos
Arabinonucleosídeos/farmacologia , Ribavirina/farmacologia , Ribonucleosídeos/farmacologia , Simplexvirus/efeitos dos fármacos , Vidarabina/farmacologia , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Coformicina/análogos & derivados , Coformicina/farmacologia , Cricetinae , Relação Dose-Resposta a Droga , Antagonismo de Drogas , Avaliação Pré-Clínica de Medicamentos , Sinergismo Farmacológico , Humanos , PentostatinaRESUMO
Exposure (10 passages) of type 1 herpesvirus to ribavirin or idoxuridine in Vero cells results in resistance to idoxuridine, but not to ribavirin.