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1.
Int J Dent ; 2022: 2316666, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36571070

RESUMO

Aim: We investigated the long-term effects of exposure to concentrated growth factors (CGFs) on the regenerative properties of dental pulp stem cells (DPSCs) in the presence and absence of lipopolysaccharide (LPS) as a proinflammatory agent. Methods: DPSCs were cultured with CGF at different concentrations of LPS (0.1, 1, and 10 µg/ml) for 21 days. Then, using MTT and scratch assays, the cell viability and migration were examined. Osteogenic stimulation was evaluated by alkaline phosphatase (ALP) staining and Sirius Red staining, which determined the ALP activity and collagen levels, respectively. The expression levels of osteogenic markers were quantified using the qRT-PCR method. One-way analysis of variance (ANOVA) and Tukey's HSD test were used to analyze differences between groups. Results: Long-term treatment of DPSCs with CGFs reduced LPS-induced cell death. Moreover, CGF and LPS (1 µg/ml), either in combination or alone, improved the DPSC migratory ability and caused a significant increase in osteogenic stimulation through the upregulation of collagen levels and ALP activity. Additionally, CGFs significantly upregulated RUNX2, DSPP, OCN, and OPN mRNA levels (as osteogenic markers), while LPS (1 µg/ml) only significantly increased OCN overexpression. Conclusion: Our findings are evidence that CGF could be a promising agent to induce dentin-pulp complex healing in long-term chronic inflammation.

2.
J Family Med Prim Care ; 11(6): 2865-2869, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36119164

RESUMO

Introduction: Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis are the two main bacteria that cause periodontitis which is an inflammatory disease of periodontal tissues. Numerous antibacterial agents have been introduced to fight against these periodontal pathogens, but the antibacterial efficacy of concentrated growth factor has not been studied yet. Therefore, this study is aimed to investigate the antibacterial impact of concentrated growth factor on the growth of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Methods: In this study, concentrated growth factor was obtained from blood samples of healthy people with no systemic disease and no antibiotics used for at least 3 months before the experiments. The concentrated growth factor from each blood sample was divided into two parts, i.e., three samples in P. gingivalis group and three samples in A. actinomycetemcomitans group, and a positive control group to verify the accuracy of the procedure and a negative group to rule out any contamination. The minimum inhibitory concentration and minimum bactericidal concentration of CGF against A. actinomycetemcomitans and P. gingivalis were determined by the broth microdilution method. Statistical analysis was performed using SPSS 22 software, and P value significance was set to 0.05. Results: The antibacterial property of CGF on the studied bacteria in concentrations of MIC and » MIC is the same for both bacteria, but in ½ MIC, its impact on A. actinomycetemcomitans is significantly higher than P. gingivalis. Examining the MBC showed that no MBC dose was obtained. Results of the disk diffusion test indicated the lack of the zones of bacterial growth inhibition. Conclusions: Considering the limitations of our study, it can be concluded that CGF has antibacterial effectiveness against A. actinomycetemcomitans and P. gingivalis with no significant difference between two bacteria.

3.
J Adv Periodontol Implant Dent ; 12(2): 95-99, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-35919751

RESUMO

Reactive lesions of soft tissue are common oral lesions that are usually non-neoplastic growths without pain; however, they can interfere with oral hygiene and plaque control, and if present in the anterior segment, they can cause esthetics problems. In this case, there was a reactive lesion at the gingiva of the left maxillary central incisor area. The particular consideration about this case was the recurrence of a peripheral ossifying fibroma as a pyogenic granuloma, indicating the association between reactive gingival lesions. The history of several recurrences due to incomplete removal shows the importance of complete excision for preventing recurrence. Therefore, the lesion was completely excised, and the mucogingival defect was successfully augmented with periodontal plastic surgery.

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