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1.
Drug Discov Today ; 28(2): 103463, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36481584

RESUMO

Long-acting injectable (LAI) delivery technologies have enabled the development of several pharmaceutical products that improve patient health by delivering therapeutics from weeks to months. Over the last decade, due to its good biocompatibility, formulation tunability, wide range of degradation rates, and extensive clinical studies, polyester-based LAI technologies including poly(lactic-co-glycolic acid) (PLGA) have made substantial progress. Herein, we discuss PLGA properties with seminal approaches in the development of LAIs, the role of molecular dynamic simulations of polymer-drug interactions, and their effects on quality attributes. We also outline the landscape of various advanced PLGA-based and a few non-PLGA LAI technologies; their design, delivery, and challenges from laboratory scale to preclinical and clinical use; and commercial products incorporating the importance of end-user preferences.


Assuntos
Simulação de Dinâmica Molecular , Poliésteres , Humanos , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Polímeros , Preparações Farmacêuticas
2.
Nat Biomed Eng ; 6(10): 1167-1179, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34980903

RESUMO

Hydrogels that provide mechanical support and sustainably release therapeutics have been used to treat tendon injuries. However, most hydrogels are insufficiently tough, release drugs in bursts, and require cell infiltration or suturing to integrate with surrounding tissue. Here we report that a hydrogel serving as a high-capacity drug depot and combining a dissipative tough matrix on one side and a chitosan adhesive surface on the other side supports tendon gliding and strong adhesion (larger than 1,000 J m-2) to tendon on opposite surfaces of the hydrogel, as we show with porcine and human tendon preparations during cyclic-friction loadings. The hydrogel is biocompatible, strongly adheres to patellar, supraspinatus and Achilles tendons of live rats, boosted healing and reduced scar formation in a rat model of Achilles-tendon rupture, and sustainably released the corticosteroid triamcinolone acetonide in a rat model of patellar tendon injury, reducing inflammation, modulating chemokine secretion, recruiting tendon stem and progenitor cells, and promoting macrophage polarization to the M2 phenotype. Hydrogels with 'Janus' surfaces and sustained-drug-release functionality could be designed for a range of biomedical applications.


Assuntos
Tendão do Calcâneo , Quitosana , Traumatismos dos Tendões , Ratos , Humanos , Suínos , Animais , Hidrogéis , Quitosana/metabolismo , Adesivos/metabolismo , Triancinolona Acetonida/metabolismo , Traumatismos dos Tendões/tratamento farmacológico , Traumatismos dos Tendões/metabolismo , Tendão do Calcâneo/metabolismo , Quimiocinas/metabolismo
3.
Adv Drug Deliv Rev ; 167: 19-46, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33202261

RESUMO

Long acting injectable formulations have been developed to sustain the action of drugs in the body over desired periods of time. These delivery platforms have been utilized for both systemic and local drug delivery applications. This review gives an overview of long acting injectable systems that are currently in clinical use. These products are categorized in three different groups: biodegradable polymeric systems, including microparticles and implants; micro and nanocrystal suspensions and oil-based formulations. Furthermore, the applications of these drug delivery platforms for the management of various chronic diseases are summarized. Finally, this review addresses industrial challenges regarding the development of long acting injectable formulations.


Assuntos
Implantes Absorvíveis , Preparações de Ação Retardada/química , Sistemas de Liberação de Medicamentos/métodos , Implantes de Medicamento/química , Química Farmacêutica , Liberação Controlada de Fármacos , Emulsões/química , Humanos , Microesferas , Nanopartículas/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Suspensões/química
4.
Biomaterials ; 178: 481-495, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29650255

RESUMO

Glucocorticoids are well established anti-inflammatory agents, however, their use to treat chronic inflammatory diseases is limited due to a number of serious side effects. For example, long-term local treatment of chronic wounds with glucocorticoids is prohibited by dysregulation of keratinocyte and fibroblast function, leading to skin thinning. Here, we developed and tested liposome formulations for local delivery of dexamethasone to primary human macrophages, to drive an anti-inflammatory/pro-resolution phenotype appropriate for tissue repair. The liposomes were loaded with the pro-drug dexamethasone-phosphate and surface-modified with either polyethylene glycol or phosphatidylserine. The latter was used to mimic phosphatidylserine-harboring apoptotic cells, which are substrates for efferocytosis, an essential pro-resolution function. Both formulations induced a dexamethasone-like gene expression signature in macrophages, decreased IL6 and TNFα release, increased secretion of thrombospondin 1 and increased efferocytosis activity. Phosphatidylserine-modified liposomes exhibited a faster uptake, a higher potency and a more robust phenotype induction than polyethylene glycol-modified liposomes. Fibroblast and keratinocyte cell cultures as well as a 3D skin equivalent model showed that liposomes applied locally to wounds are preferentially phagocytosed by macrophages. These findings indicate that liposomes, in particular upon shell modification with phosphatidylserine, promote dexamethasone delivery to macrophages and induce a phenotype suitable to support chronic wound healing.


Assuntos
Glucocorticoides/farmacologia , Macrófagos/patologia , Cicatrização/efeitos dos fármacos , Células Cultivadas , Microambiente Celular/efeitos dos fármacos , Dexametasona/análogos & derivados , Dexametasona/farmacologia , Endocitose/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Fluorescência , Humanos , Inflamação/patologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/patologia , Cinética , Lipossomos , Macrófagos/efeitos dos fármacos , Fagocitose/efeitos dos fármacos , Fenótipo , Fosfatidilserinas/química , Polietilenoglicóis/química
5.
Mol Pharm ; 10(1): 319-28, 2013 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-23259920

RESUMO

We present an in situ hydrophobic salt forming technique for the encapsulation of weakly hydrophobic, ionizable active pharmaceutical ingredients (API) into stable nanocarriers (NCs) formed via a rapid precipitation process. Traditionally, NC formation via rapid precipitation has been difficult with APIs in this class because their intermediate solubility makes achieving high supersaturation difficult during the precipitation process and the intermediate solubility causes rapid Ostwald ripening or recrystallization after precipitation. By forming a hydrophobic salt in situ, the API solubility and crystallinity can be tuned to allow for NC formation. Unlike covalent API modification, the hydrophobic salt formation modifies properties via ionic interactions, thus circumventing the need for full FDA reapproval. This technique greatly expands the types of APIs that can be successfully encapsulated in NC form. Three model APIs were investigated and successfully incorporated into NCs by forming salts with hydrophobic counterions: cinnarizine, an antihistamine, clozapine, an antipsychotic, and α-lipoic acid, a common food supplement. We focus on cinnarizine to develop the rules for the in situ nanoprecipitation of salt NCs. These rules include the pK(a)s and solubilities of the API and counterion, the effect of the salt former-to-API ratio on particle stability and encapsulation efficiency, and the control of NC size. Finally, we present results on the release rates of these ion pair APIs from the NCs.


Assuntos
Portadores de Fármacos/química , Nanopartículas/química , Polímeros/química , Precipitação Química , Cinarizina/química , Clozapina/química , Estabilidade de Medicamentos , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Íons/química , Naftóis/química , Tamanho da Partícula , Sais/química , Solubilidade , Ácido Tióctico/química
6.
Anesthesiology ; 107(3): 442-51, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17721247

RESUMO

BACKGROUND: High-molecular-weight, low-substituted hydroxyethyl starch (HES) may not affect blood coagulation more than low-molecular-weight, low-substituted HES. The authors assessed in vivo the effect of a lowered C2/C6 ratio on pharmacokinetic characteristics and the impact on blood coagulation of high-molecular-weight, low-substituted HES. METHODS: A prospective, randomized, parallel study in 30 pigs compared HES 650/0.42/2.8 with HES 650/0.42/5.6. Before, during, and after infusion of 30 ml/kg body weight HES, blood samples were collected over 630 min to measure HES concentrations and plasmatic coagulation and to assess blood coagulation in whole blood by Thrombelastography (TEG; Haemoscope Corporation, Niles, IL). Pharmacokinetic parameters were estimated using a two-compartment model. RESULTS: The elimination constant was 0.009 +/- 0.001 min(-1) for HES 650/0.42/2.8 and 0.007 +/- 0.001 min(-1) for HES 650/0.42/5.6 (P < 0.001); the area under the plasma concentration-time curve was 1,374 +/- 340 min x g/l for HES 650/0.42/2.8 and 1,697 +/- 411 min x g/l for HES 650/0.42/5.6 (P = 0.026). The measured plasma HES concentrations were not different between HES 650/0.42/2.8 and HES 650/0.42/5.6. Both HES solutions equally affected blood coagulation: Thrombelastographic coagulation index decreased similarly at the end of infusion of HES 650/0.42/2.8 and at the end of infusion of HES 650/0.42/5.6 (P = 0.293). Also, activated partial thromboplastin and prothrombin times increased similarly for HES 650/0.42/2.8 and HES 650/0.42/5.6 (P = 0.831). CONCLUSION: Reducing the C2/C6 ratio in high-molecular, low-substituted HES solutions results in a slightly faster HES elimination. However, the blood coagulation compromising effect was unaffected.


Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Derivados de Hidroxietil Amido/farmacocinética , Substitutos do Plasma/farmacocinética , Albuminas/efeitos dos fármacos , Animais , Área Sob a Curva , Testes de Coagulação Sanguínea , Hemoglobinas/efeitos dos fármacos , Derivados de Hidroxietil Amido/sangue , Derivados de Hidroxietil Amido/química , Peso Molecular , Substitutos do Plasma/química , Substitutos do Plasma/metabolismo , Estudos Prospectivos , Distribuição Aleatória , Relação Estrutura-Atividade , Suínos , Tromboelastografia/métodos , Fatores de Tempo
7.
Anesthesiology ; 106(1): 132-43, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17197855

RESUMO

BACKGROUND: Carboxymethyl starch (CMS) and carboxymethylated hydroxyethyl starch (CM-HES) might offer advantages over hydroxyethyl starch (HES) with regard to their volume expansion effect and their pharmacokinetic characteristics. The goal of the current study was to determine the pharmacokinetics of CMS and CM-HES and to investigate their influence on blood coagulation in comparison with the standard low-molecular, low-substituted HES (130/0.42) used in Europe. METHODS: The study was conducted as a randomized, blinded, parallel three-group study in 30 pigs. Twenty ml/kg of 6% HES (control), 6% CMS, or 6% CM-HES was infused as a single dose, and serial blood sampling was performed over 20 h to measure plasma concentration and molecular weight and to assess blood coagulation. Concentration-effect relations were assessed by pharmacokinetic-pharmacodynamic analysis. RESULTS: CMS and CM-HES showed significantly higher plasma concentrations and molecular weights over 20 h (P for both<0.001) with smaller volumes of distribution and longer elimination rates during the terminal phase (P for both<0.01) when compared with HES. CMS and CM-HES impaired whole blood coagulation more than HES as assessed by Thrombelastograph analysis (Haemoscope Corporation, Niles, IL). However, similar effects of all three starch preparations on blood coagulation were found when related to the plasma concentrations in mass units. CONCLUSIONS: Carboxymethylation of starch results in an increased intravascular persistence and a slower fragmentation compared with HES. The greater impairment of blood coagulation by CMS and CM-HES seems to be caused by the higher plasma concentrations.


Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Substitutos Sanguíneos/farmacocinética , Derivados de Hidroxietil Amido/farmacocinética , Amido/análogos & derivados , Animais , Coloides , Hemodiluição , Hemoglobinas/análise , Derivados de Hidroxietil Amido/farmacologia , Pressão Osmótica , Amido/farmacocinética , Amido/farmacologia , Suínos
8.
Anesthesiology ; 105(6): 1228-37, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17122586

RESUMO

BACKGROUND: Hydroxyethyl starches (HES) with lower impact on blood coagulation but longer intravascular persistence are of clinical interest. The current study aimed to investigate in vivo the isolated effect of molecular weight on blood coagulation during progressive acute normovolemic hemodilution. METHODS: Twenty-four pigs were normovolemically hemodiluted up to a total exchange of 50 ml . kg . body weight of HES 650/0.42 or HES 130/0.42. Serial blood sampling was performed to measure HES plasma concentration and to assess blood coagulation. Concentration-effect relations were analyzed by linear regression, followed by the Student t test on regression parameters. RESULTS: Blood coagulation was increasingly compromised toward hypocoagulability by acute normovolemic hemodilution with both treatments (P < 0.01). Significantly greater impact on activated partial thromboplastin time (P = 0.04) and significantly stronger decrease of maximal amplitude (P = 0.04), angle alpha (P = 0.02), and coagulation index (P = 0.02) was seen after acute normovolemic hemodilution with HES 650/0.42 as compared with HES 130/0.42. Except for factor VIII (P = 0.04), no significant differences between both treatments were observed when relating antihemostatic effects to HES plasma concentrations (P > 0.05). A significantly lesser decrease of hemoglobin concentration has been found with HES 650/0.42 as compared with HES 130/0.42 (P < 0.01) in relation to HES plasma concentrations. CONCLUSION: High-molecular-weight HES (650/0.42) shows a moderately greater antihemostatic effect than low-molecular-weight HES (130/0.42) during acute normovolemic hemodilution. However, similar effects on hemostasis were observed with both treatments when observed antihemostatic effects were related to measured HES plasma concentrations. In addition, HES 650/0.42 may have a lower efficacy in immediately restoring plasma volume.


Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Hemodiluição , Derivados de Hidroxietil Amido/química , Derivados de Hidroxietil Amido/farmacologia , Substitutos do Plasma/química , Substitutos do Plasma/farmacologia , Animais , Viscosidade Sanguínea , Derivados de Hidroxietil Amido/farmacocinética , Peso Molecular , Tempo de Tromboplastina Parcial , Substitutos do Plasma/farmacocinética , Tempo de Protrombina , Respiração Artificial , Suínos , Tromboelastografia
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