Protein metabolism in the synovial membrane in the hip osteoarthritis.

BACKGROUND: Osteoarthritis (OA), and particularly osteoarthritis of the hip (coxarthrosis), together with hypertension and diabetes, belong to the most common lifestyle diseases. Usually the determination of a clear reason for the degenerative changes is impossible. However, the role of reactive oxygen species (ROS) that can affect the protein metabolism and thus cause the degradation of the joint connective tissue structures has been recently frequently pointed out. The aim of this study was to compare the activity of selected enzymes and albumin and protein level in the synovial membrane obtained during hip replacement surgery due to coxarthrosis. MATERIAL/METHODS: The reference group consisted of patients after traumatic femoral neck fracture, without osteoarthritis in the medical record. Collected sections of the synovial membrane were prepared for biochemical tests according to the authors' methodology. RESULTS: In the synovial membrane of patients with hip osteoarthritis significantly higher activity of superoxide dismutase (SOD) and glutamate dehydrogenase (GLDH) was observed, as well as increased activity of prolidase and lower activity of acid phosphatase activity compared with the reference group. The concentration of total protein and albumin in the synovial membrane was similar in both groups. CONCLUSIONS: Increased SOD activity indirectly indicates ROS participation in OA pathogenesis. Coexisting protein metabolism disorders of the synovial membrane may contribute to the degradation of the articular cartilage.

Metabolism and protein transformations in synovial membrane of a knee joint in the course of rheumatoid arthritis and degenerative arthritis.

BACKGROUND: In the course of musculoskeletal system diseases, such as rheumatoid arthritis and degenerative arthritis a chronic inflammatory process develops, which deteriorates all the joint elements and leads to the movement insufficiency of a patient. In case of both of theses diseases, etiology is multi-factor and still not known thoroughly. It is suggested that in the process of degradation of a joint cartilage, active form of oxygen take part. Their excessive production contributes to oxidation imbalances in cells and an oxidative stress. Under the activity of fee radicals, among others, activation of proteolytic enzymes participating the collagen degradation starts. The aim of this work is to compare parameters characteristic a cell metabolism and protein transformations taking place in the course of the aforementioned musculoskeletal system diseases. MATERIAL/METHODS: The material tested consisted of fragments of synovial membrane of a knee joint taken from 36 women suffering from rheumatoid arthritis and 24 women suffering from osteoarthritis during the procedure of knee-joint endoprothesoplastic surgery. Then the material was subject to the author's methodology of preparations of synovial membrane for biochemical markings. RESULTS: In the group of patients suffering from rheumatoid arthritis significantly higher protein and sulfhydryl groups concentrations were achieved. Moreover, an increase of activity of manganese isoenzyme of superoxide dismutase, glutamate dehydrogenase and enzymes participating in the process of collagen degradation--prolidase and acid phosphatase was observed. CONCLUSIONS: In the course of rheumatoid arthritis a speed of cell metabolism increase, which leads to a higher intensity of protein turnover in cells.