RESUMO
Lymphoid cells obtained from spleens of patients with lymphomas or leukemias were studied for the presence of heterophile (Paul-Bunnell (P-B)) antigen. A mixed agglutination (MA) test was established utilizing monolayers of cells attached to poly-L-lysine-coated wells of plastic U plates. After incubation of the monolayers with infectious mononeucleosis (IM) sera, indicator cells, sheep, or trypsinized bovine erythrocytes were added. The results were assessed according to sedimentation patterns of the indicator cells on the monolayers. Positive MA reactions were shown to be due to specific binding of P-B antibodies to the corresponding antigens on the spleen cells. Positive results were obtained with 15 of 37 spleens from patients with Hodgkin's disease, 5 of 8 lymphoma spleens, 4 of 15 chronic myelocytic leukemia spleens and 2 of 4 chronic lymphocytic leukemia spleens. Only 2 of 25 spleens from patients with various other diseases and 1 of 26 apparently normal thymus specimens gave positive results. This study confirmed demonstration of P-B antigen in lymphoma and leukemia by means of absorption experiments, which was reported previously.
Assuntos
Antígenos , Mononucleose Infecciosa/imunologia , Leucemia/imunologia , Linfoma/imunologia , Baço/imunologia , Testes de Aglutinação , Especificidade de Anticorpos , Doença de Hodgkin/imunologia , Humanos , Soros Imunes/farmacologia , Leucemia Mieloide/imunologiaAssuntos
Anticorpos Antineoplásicos/análise , Antígenos de Neoplasias/análise , Neoplasias/imunologia , Imunologia de Transplantes , Animais , Anticorpos Heterófilos , Especificidade de Anticorpos , Bovinos/imunologia , Epitopos , Eritrócitos/imunologia , Cavalos/imunologia , Humanos , Mononucleose Infecciosa/imunologia , Leucemia/imunologia , Linfoma/imunologia , Transplante de Neoplasias , Ovinos/imunologia , Neoplasias Esplênicas/imunologiaAssuntos
Antígenos Heterófilos/análise , Soros Imunes , Mononucleose Infecciosa/imunologia , Leucemia/imunologia , Linfoma/imunologia , Baço/imunologia , Absorção , Animais , Anticorpos Heterófilos/análise , Bovinos/imunologia , Eritrócitos/imunologia , Técnica de Placa Hemolítica , Linfócitos/imunologia , Baço/citologiaRESUMO
Extracts of cultured human leukemic tissues increased the spleen focus-forming activity of Friend leukemia virus preparations in BALB/c and other partially resistant mice. Such mice carry the Fv-1(b) gene, which inhibits the expression of helper virus indigenous to the Friend virus complex, and allows co-infecting leukemia viruses of mice, cats, or chickens to substitute for the inhibited helper virus and increase the expression of the spleen focus-forming virus. The helper activity of extracts from human leukemic tissues shared several important properties with that associated with known leukemogenic viruses of animals.