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1.
Electrophoresis ; 26(13): 2511-9, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15948211

RESUMO

Diaminocarboxylic acid carrier ampholytes, such as L-histidine, 2,3-diaminopropionic acid, L-ornithine, and L-lysine, were reacted with glycerol-1,3-diglycidyl ether (GDGE) and poly(vinyl alcohol) (PVA) in the presence of sodium hydroxide to produce hydrolytically and mechanically stable hydrogels, supported on a PVA substrate, for use as buffering membranes in isoelectric trapping (IET) separations. The pH values of the DACAPVA membranes were determined with the help of small-molecule pI markers and proteins and were found to be in the 6 < pH < 8.5 range. The membranes were successfully used to isoelectrically trap small ampholytes, desalt ampholyte solutions in IET mode, and effect the binary separation of chicken egg white proteins.


Assuntos
Focalização Isoelétrica/métodos , Membranas Artificiais , Animais , Galinhas , Proteínas do Ovo/isolamento & purificação , Compostos de Epóxi/química , Éteres de Glicerila/química , Hidrogéis/síntese química , Concentração de Íons de Hidrogênio , Ponto Isoelétrico , Álcool de Polivinil/química
2.
Electrophoresis ; 25(14): 2128-38, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15273996

RESUMO

Alkali-stable, high-pI isoelectric membranes have been synthesized from quaternary ammonium derivatives of cyclodextrins and poly(vinyl alcohol), and bifunctional cross-linkers, such as glycerol-1,3-diglycidyl ether. The new, high-pI isoelectric membranes were successfully applied as cathodic membranes in isoelectric trapping separations in place of the hydrolytically more labile, polyacrylamide-based cathodic isoelectric membranes, and permitted the use of catholytes as alkaline as 1 M NaOH. The new high-pI isoelectric membranes have shown excellent mechanical stability, low electric resistance and long life times, even when subjected to electrophoresis with current densities as high as 80 mA/cm2.


Assuntos
Reagentes de Ligações Cruzadas/química , Ciclodextrinas/química , Eletroforese/instrumentação , Membranas/química , Álcool de Polivinil/química
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