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2.
Bioanalysis ; 7(21): 2731-4, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26563751

RESUMO

This Land O'Lakes Conference is presented each year by the Division of Pharmacy Professional Development within the School of Pharmacy at the University of Wisconsin-Madison (USA). The purpose of this 3-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis. To increase the interactive nature of the conference, the program is a mixture of lectures, interactive discussions and a poster session. This report summarized the presentations at the 16th Annual Conference. 6th Annual Land O'Lakes Bioanalytical Conference, Fluno Center Madison, WI, USA, 13-16 July 2015.


Assuntos
Produtos Biológicos/análise , Biomarcadores/análise , Preparações Farmacêuticas/análise , Animais , Congressos como Assunto , Humanos , Espectrometria de Massas/normas
3.
Bioanalysis ; 6(21): 2915-8, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25486236

RESUMO

This Land O'Lakes Conference is presented each year by the Division of Pharmacy Professional Development within the School of Pharmacy at the University of Wisconsin-Madison (USA). The purpose of this 3-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis. To increase the interactive nature of the conference, the program is a mixture of lectures, interactive discussions and a poster session. This report summarized the presentations at the Fifteenth Annual Conference.


Assuntos
Biomarcadores/análise , Animais , Produtos Biológicos/análise , Cromatografia Líquida de Alta Pressão/normas , Regulamentação Governamental , Humanos , Espectrometria de Massas/normas , Xenobióticos/análise
4.
Bioanalysis ; 5(21): 2601-5, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24180501

RESUMO

The University of Wisconsin bioanalytical conference is presented each year by the Extension Services in Pharmacy, the professional development department within the School of Pharmacy. The purpose of this 3-day conference was to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference was designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis. To increase the interactive nature of the conference the program was composed of a mixture of lectures, interactive discussions, poster sessions and roundtables. This paper summarizes the presentations at the Fourteenth Annual Conference, offered in a new venue.


Assuntos
Produtos Biológicos/análise , Xenobióticos/análise , Biomarcadores/análise , Química Analítica , Humanos
5.
Bioanalysis ; 4(21): 2557-61, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23173791

RESUMO

This University of Wisconsin School of Pharmacy bioanalytical conference is presented each year by the Extension Services in Pharmacy, the professional development department within the School. The purpose of this 4-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis. To increase the interactive nature of the conference, the program was a mixture of lectures, poster sessions, round table discussions and workshops. This article summarizes the presentations at the 13th Annual Conference.


Assuntos
Biomarcadores/análise , Técnicas de Química Analítica , Xenobióticos/análise , Animais , Humanos
6.
Bioanalysis ; 3(19): 2171-5, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21985412

RESUMO

This University of Wisconsin School of Pharmacy bioanalytical conference is presented each year by the Extension Services in Pharmacy, the professional development department within the school. The purpose of this 4-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis. To increase the interactive nature of the conference, the program was a mixture of lectures, poster sessions, round table discussions and workshops. This article summarizes the presentations at the 12th Annual Conference.


Assuntos
Biomarcadores/análise , Técnicas de Química Analítica , Química Analítica/tendências , Humanos , Xenobióticos/análise
7.
J Pharm Biomed Anal ; 48(3): 866-75, 2008 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-18692977

RESUMO

Tigecycline (Tygacil,Wyeth) is a first-in-class, broad spectrum antibiotic with activity against multiple-resistant organisms. In order to address the unexpectedly low tigecycline concentrations in human bone samples analyzed using a LC/MS/MS method developed elsewhere, we have developed and validated a new and sensitive human bone assay for the quantitation of tigecycline using LC/MS/MS. The new method utilizes the addition of a stabilizing agent to the human bone sample, homogenization of human bone in a strong acidic-methanol extraction solvent, centrifugation of the bone suspension, separation by liquid chromatography, and detection of tigecycline by mass spectrometry. Linearity was demonstrated over the concentration range from 50 to 20,000 ng/g using a 0.1g human bone sample. The intra- and inter-day accuracy of the assay was within 100+/-15%, and the corresponding precision (CV) was <15%. The stability of tigecycline was evaluated and shown to be acceptable under the assay conditions. The extraction recovery of tigecycline with the current method was 79% when using radio-labeled rat bone samples as a substitute for human bone samples. Twenty-four human bone samples collected previously from volunteers without infections who had elective orthopedic surgery after receiving a single dose of tigecycline were re-analyzed using the current validated method. Tigecycline concentrations in these samples ranged from 238 to 794 ng/g with a mean value 9 times higher than the mean concentration previously reported. The data demonstrated that the current method has significantly higher extraction efficiency than the previously reported method.


Assuntos
Antibacterianos/análise , Osso e Ossos/química , Cromatografia Líquida/métodos , Minociclina/análogos & derivados , Espectrometria de Massas em Tandem/métodos , Animais , Antibacterianos/química , Bioensaio , Calibragem , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Estabilidade de Medicamentos , Humanos , Concentração de Íons de Hidrogênio , Minociclina/análise , Minociclina/química , Estrutura Molecular , Controle de Qualidade , Ensaios Clínicos Controlados Aleatórios como Assunto , Ratos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Solventes/química , Tigeciclina
8.
J Pharm Biomed Anal ; 44(4): 970-9, 2007 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-17537611

RESUMO

Tigecycline (Tygacil) is a first-in-class, broad spectrum antibiotic with activity against antibiotic-resistant organisms. In rats and humans, tigecycline readily distributes to bone tissue but its accuracy of quantitation via liquid chromatography/mass spectrometry (LC/MS/MS) is hindered by a low extraction recovery when using a conventional plasma extraction method. To overcome this issue, we have identified an effective extraction solvent for quantitation of tigecycline in rat bone. The current LC/MS/MS bone assay is novel, simple, and sensitive, and has a wide linear range of 50-10,000 ng/g. The assay requires homogenization of the rat bone in a strong acidic-methanol extraction solvent, centrifugation of the bone suspension, separation of the supernatant with liquid chromatography, and detection of tigecycline with tandem mass spectrometry. The incurred pooled rat bone samples obtained from rats given 3mg/kg/day of [(14)C]-tigecycline and non-radio-labeled tigecycline were analyzed with the current method. The absolute extraction recovery of the bone assay for tigecycline was 77.1%. The intra-day accuracy ranged from 91.7 to 106% with precision (CV) of 1.9-10.7%, and inter-day accuracy ranged from 96.1 to 100% with a precision of 6.3-8.7%. In addition, biological activity was demonstrated for the tigecycline extracted from incurred rat bone. This bone assay provides an important analytical tool for the determination of drug concentrations (especially, antimicrobials) in rodent bone tissues and has served as the foundation of development and validation of a similar bone assay for tigecycline in human bone tissues.


Assuntos
Antibacterianos/análise , Osso e Ossos/química , Minociclina/análogos & derivados , Animais , Bacillus cereus/efeitos dos fármacos , Bioensaio , Calibragem , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Interpretação Estatística de Dados , Masculino , Minociclina/análise , Controle de Qualidade , Ratos , Ratos Sprague-Dawley , Soluções , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem , Tigeciclina
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