The LTAR Cropland Common Experiment at the Texas Gulf.

Texas Gulf is one of the 18 regional sites that is part of the USDA-ARS Long-Term Agroecosystem Research (LTAR) Network and focuses on cropland and integrated grazing land research in Central Texas, addressing challenges posed by soil characteristics, climate variability, and urbanization. This paper provides brief site descriptions of the two Cropland Common Experiments being conducted in the Texas Gulf LTAR region, emphasizing conservation tillage practices and precision agriculture techniques. The plot-scale study is located in Temple, TX, at the USDA-ARS Grassland, Soil and Water Research Laboratory and examines conventional tillage, strip tillage, and no tillage practices. The field-scale study, located in Riesel, TX, at the USDA-ARS Riesel Watersheds, assesses the impact of no tillage, cover crops, fertility management, adaptive management, and precision conservation on crop yield, profitability, and environmental footprint. Key measurements include soil and plant analyses, greenhouse gas fluxes, runoff water quantity and quality, and field operations recorded with precision agriculture equipment. Despite challenges posed by urban encroachment, future research aims to incorporate new technologies, such as unmanned ground vehicles, to enhance sustainability and productivity of the agricultural landscape. These experiments provide valuable insights for stakeholders, contributing to the development of sustainable agricultural practices tailored to the unique challenges within the Texas Gulf LTAR region.

The LTAR-integrated grazing land common experiment at the Texas Gulf.

Extreme weather and climate events have become more frequent and directly affect the ecological structure and function of integrated grazing lands. While the Great Plains have experienced a long history of regular disturbances from drought and floods, grazing, and fires, the increased frequency and magnitude of these disturbances can reduce ecological resilience, largely depending on management practices. Alternative strategies designed to adaptively manage grazing land resources based on the ecology of the system should increase the resistance and resilience to disturbances when compared to prevailing practices. Determining the ecologic and economic value of alternative strategies will require long-term evaluations across large spatial scales. The Long-Term Agroecosystem Research Network has been established to evaluate the differences between alternative and prevailing practices among 18 strategically located sites and across decadal time scales throughout the continental United States. A key integrated grazing land site within this network is the Texas Gulf located at the Riesel Watersheds in the Blackland Prairie of Central Texas. At this study site, the differences between alternative and prevailing grazing management strategies are now being evaluated. The alternative strategy was designed using a combination of knowledge of the site and species ecology with modern-day tools and technologies. Alternatively, the prevailing practice implements a conventional year-round continuous grazing system with heavy reliance on hay and supplemental protein during winter. Results will provide grazing land managers with economically viable adaptive management choices for increasing ecological resilience following extreme and frequent disturbance events.

Myocardial plasma cell tumor with intratumoral amyloidosis in a dog.

A 12-year-old mixed breed dog was evaluated for marked hypercalcemia that was identified during assessment for acute polydipsia and polyuria. Physical examination identified a new grade II/VI left apical systolic murmur. A mass involving the left ventricular posterior wall and left atrium was identified by echocardiography, suggesting neoplastic invasion into the myocardium. The patient was euthanized, and post-mortem cardiac evaluation identified an intramyocardial amyloid-producing plasma cell tumor. Multiple myeloma was suspected but could not be confirmed due to the limited post-mortem evaluation. This case is the first report of myocardial amyloidosis in a dog with a myeloma-related disorder (MRD). Dogs with MRD and myocardial involvement may not exhibit clinical signs that localize to the cardiovascular system; therefore, echocardiography should be considered during the staging process.

A site suitability analysis for castor (Ricinus communis L.) production during Brazil's second harvest incorporating disease prediction.

Castor (Ricinus communis L.) is an important industrial crop with a wide range of industrial and pharmaceutical applications. Brazil is among the largest castor-producing countries. Between 2004 and 2010, castor cultivation was stimulated with an emphasis towards biodiesel production. However, this was not enough to leverage the production of castor in Brazil, mainly due to the lack of structured trade and the competition with other cheaper raw materials for the production of biodiesel. Despite this failure, the species presents itself as an excellent alternative for crop rotation in the second crop among soybean, corn, beans, and cotton cultivation areas as the oil is highly valuable for other products. Moreover, it has drawn the attention of producers and researchers in Brazil for this potential rotation as it is considered a plant tolerant of water-deficiency and is highly susceptible to gray mold, a disease favored by high humidity in the final stages of the crop. For instance, its cultivation in the second crop in Cerrado regions, where rains occur in the early stages of the crop and cease when the plants reach the final stage of production, has been successful and shows great promise. The current study aimed to evaluate the suitability of environments throughout Brazil to grow castor, incorporating variables associated with the incidence of gray mold and confirm these findings based on existing castor trial data obtained from the literature. The site suitability analysis determined that 74.99 million hectares - 8.8% of Brazilian territory - are highly suitable for castor production during second harvest, mostly located in the Northeastern and Midwestern regions. These results are surprising since Brazil currently has around 7.8% (∼66.81 million hectares) of its territory occupied with agriculture (grains, fruits, vegetables, and perennial crops). The findings of this study provide a method to perform site suitability for crops using data associated with agronomic and disease characteristics, as is the case with gray mold that often results in significant losses in castor production. Also, this analysis provides evidence for the great potential of Brazil to increase castor production and meet the world demand for its oil through utilization of second-crop cultivation.

Associations Between Primary Residence and Mental Health in Global Marginalized Populations.

Scholars suggest that marginalized people in non-urban areas experience higher distress levels and fewer psychosocial resources than in urban areas. Researchers have yet to test whether precise proximity to urban centers is associated with mental health for marginalized populations. We recruited 1733 people who reported living in 45 different countries. Participants entered their home locations and completed measures of anxiety, depression, social support, and resilience. Regression and thematic analyses were used to determine what role distance from legislative and urban centers may play in mental health when marginalized people were disaggregated. Greater distance from legislative center predicted higher anxiety and resilience. Greater distance from urban center also predicted more resilience. Thematic analyses yielded five categories (e.g., safety, connection) that further illustrated the impact of geographic location on health. Implications for community mental health are discussed including the need to better understand and further expand resilience in rural areas.

Air Pollution Levels Outside the Capital's Maternity Hospitals.

Introduction The World Health Organisation has identified air pollution as the single biggest environmental threat to human health. There is growing evidence in the literature that air pollution is associated with negative outcomes in pregnancy. The purpose of this study was to measure pollution levels in the immediate surroundings of the three Dublin maternity hospitals by measuring fine particulate matter <2.5 micrometres (PM2.5). Methods Data pertaining to levels of PM2.5 at the three Dublin maternity hospitals were obtained from Pollutrack's records for the time period 25/6/2021-2/12/2021. Results were compared to the 2021 WHO Air Quality Guidelines. Results Average PM2.5 levels were 9µg/m³ around the National Maternity Hospital, 10µg/m³ around the Coombe Hospital and 13µg/m³ around the Rotunda Hospital. Levels were higher during the day, weekdays and in December. No matter when the PM2.5 levels were measured, results were higher than those recommended by the World Health Organisation's Air Quality Guideline. Discussion Air pollution levels across Ireland's capital city are higher than recommended by the WHO. This is concerning for the public and in particular for the pregnant population. Going forward, further research is required on the relationship between levels of air pollutants and adverse pregnancy outcomes in Dublin.

Opportunities to implement manureshed management in the Iowa, North Carolina, and Pennsylvania swine industry.

The U.S. swine industry is diverse, but opportunities exist to strategically improve manure management, especially given much of the industry's vertical integration. We investigate opportunities for improving manureshed management, using swine production examples in Iowa, North Carolina, and Pennsylvania as a lens into historical trends and the current range of management conditions. Manure management reflects regional differences and the specialized nature of hog farms, resulting in a large range of land bases required to assimilate manure generated by these operations. Selected representative farm scenarios were evaluated on an annual basis; farm-level manuresheds were largest for Pennsylvania sow farms and smallest for North Carolina nursery farms. Compared with nitrogen-based manuresheds, phosphorus-based manuresheds were up to 12.5 times larger. Technology advancements are needed to promote export of concentrated nutrients, especially phosphorus, from existing "source" manuresheds to suitable croplands. The industry is dynamic, as revealed by historical analysis of the siting of hog barns in Pennsylvania, which are currently trending toward the north and west where there is greater isolation to prevent the spread of disease and a larger land base to assimilate manure. Industry expansion should focus on locating animals in nutrient "sink" areas.

Challenges and opportunities for manureshed management across U.S. dairy systems: Case studies from four regions.

The manureshed represents cropland needed to safely assimilate manure nutrients from an animal feeding operation. Dairy manuresheds can be contained on-farm but may need to involve additional farms that can assimilate excess nutrients. We present case studies reviewing challenges and opportunities to manureshed management in four major dairy-producing states using available information on local manuresheds. Additionally, geographic information system software was used with data from regulated Minnesota dairies to assess cropland assimilative capacities and transport needs surrounding large dairies. Manureshed requirements vary across regions, but increased import of feed and soil phosphorus accumulation constrain on-farm manure utilization across the United States. In Minnesota, a growing proportion of Jersey cattle and differences in continuous corn (Zea mays L.) vs. corn-alfafa (Medicago sativa L.) rotations contribute to the amount of land needed to absorb dairy manure nutrients. Farm-gate budgets reveal that N-based manuresheds can be contained within Idaho dairies, but P-based manuresheds extend beyond the farm. In New Mexico, relocation of surplus manure nutrients off the farm is common via informal networks, but incentives to strengthen these networks could ensure sustainable manureshed management. Evaluation of manureshed requirements in Pennsylvania is often complicated by the need for additional nutrient management planning and greater understanding of nutrient balances on the preponderance of small dairies. Nutrient imbalances with highly concentrated dairy production often lead to the need for manure transport off-farm. However, advances in herd and cropland management offer opportunities to improve on-farm nutrient efficiencies, and emerging networks and technologies promise to facilitate manure export when needed.

DEVELOPING INTEGRAL PROJECTION MODELS FOR ECOTOXICOLOGY.

In many ecosystems, especially aquatic ecosystems, size plays a critical role in the factors that determine an individual's ability to survive and reproduce. In aquatic ecotoxicology, size informs both realized and potential acute and chronic effects of chemical exposure. This paper demonstrates how chemical and nonchemical effects on growth, survival, and reproduction can be linked to population-level dynamics using size-structured integral projection models (IPM). The modeling approach was developed with the goals and constraints of ecological risk assessors in mind, who are tasked with estimating the effects of chemical exposures to wildlife populations in a data-limited environment. The included case study is a collection of daily time-step IPMs parameterized for the life history and annual cycle of fathead minnows (Pimephales promelas), which motivated the development of modeling techniques for seasonal, iteroparous reproduction, density dependent growth effects, and size-dependent over-winter survival. The effects of a time-variable annual chemical exposure were interpreted using a toxicokinetic-toxicodynamic model for acute survival and sub-lethal growth effects model for chronic effects and incorporated into the IPMs. This paper presents a first application of integral projection models to ecotoxicology. Our research demonstrates that size-structured IPMs provide a promising, flexible, framework for synthesizing ecotoxicologically relevant data and theory to explore the effects of chemical and nonchemical stressors and the resulting impacts on exposed populations.

Land use change and collaborative manureshed management in New Mexico.

Agricultural communities of New Mexico regularly redistribute manure nutrients from dairies to nearby croplands to fulfill agronomic nutrient needs and protect water quality. Yet competition for water resources can result in land use change that affects these cooperative manure transfers. Focusing on three clusters of New Mexico dairy farms and their surrounding lands (three manuresheds), we calculated the magnitude of land use changes in 2008-2019 and the balance between manure nutrient supply and crop demand in 2019 to assess how past change may predict future prospects for sustainable management. The overall magnitude of change was small, with each manureshed experiencing a different complement: an exchange of cropland and rangeland in the Roosevelt manureshed (7,975 ha rangeland to cropland; 7,624 ha cropland to rangeland), a 464-ha gain in cropland but a 1,187-ha loss of "spreadable" land (cropland, rangeland, fallow) to developed land in the Doña Ana manureshed, and relatively minor changes in the Chaves manureshed. Nutrient supply and demand were mainly in balance, but a surplus of manure phosphorus (P) in the Chaves manureshed and a thin margin of P assimilation by croplands in the Roosevelt manureshed point to the need for preserving existing croplands and understanding of effects of dairy manure on shortgrass rangeland. Our assessment suggests that an ideal scenario would entail manure being generated in landscapes with portfolios of productive lands that can sustainably use the manure nutrients to minimize environmental quality concerns and agronomic tradeoffs. Coordinated, participatory, and interdisciplinary research and planning are needed.

Poultry manureshed management: Opportunities and challenges for a vertically integrated industry.

Manureshed management seeks to address systemic imbalances in nutrient distributions at scales beyond the farmgate and potentially across county and state boundaries. The U.S. poultry industry, which includes broilers, layers, pullets, and turkeys, has many characteristics that are compatible with achieving a vision of manureshed management, including a history of engaging in local and regional programs to better distribute manure resources. Despite widespread vertical integration that supports large-scale strategic decision making and dry manures that favor off-farm transport, there are still many challenges to poultry manureshed management that require engaging stakeholders other than just the poultry industry. Analysis of county-level nutrient budgets highlights the industry's "mega-manureshed," extending from the Mid-Atlantic, across the southeast, and into northwest Arkansas, Oklahoma, and Texas. The analysis also identifies areas with legacy nutrient build-up that are still present today. Implementing manureshed management in the U.S. poultry industry requires comprehensive consideration of manure treatment technologies, alternative uses such as bioenergy production, market development for treated manure products, transport of manure nutrients from source to sink areas, and manure brokering programs that promote manure nutrient distribution. Fortunately, past and present evolution and innovation within the industry places it as a likely leader of the manureshed vision.

Genetic variability and population structure of Ethiopian chickpea (Cicer arietinum L.) germplasm.

Evaluation of the genetic diversity and an understanding of the genetic structure and relationships of chickpea genotypes are valuable to design efficient germplasm conservation strategies and crop breeding programs. Information is limited, in these regards, for Ethiopian chickpea germplasms. Therefore, the present study was carried out to estimate the genetic diversity, population structure, and relationships of 152 chickpea genotypes using simple sequence repeats (SSR) markers. Twenty three SSR markers exhibited polymorphism producing a total of 133 alleles, with a mean of 5.8 alleles per locus. Analyses utilizing various genetic-based statistics included pairwise population Nei's genetic distance, heterozygosity, Shannon's information index, polymorphic information content, and percent polymorphism. These analyses exemplified the existence of high genetic variation within and among chickpea genotypes. The 152 genotypes were divided into two major clusters based on Nei's genetic distances. The exotic genotypes were grouped in one cluster exclusively showing that these genotypes are distinct to Ethiopian genotypes, while the patterns of clustering of Ethiopian chickpea genotypes based on their geographic region were not consistent because of the seed exchange across regions. Model-based population structure clustering identified two discrete populations. These finding provides useful insight for chickpea collections and ex-situ conservation and national breeding programs for widening the genetic base of chickpea.

Geographic Distributions of Drag Performers Relative to Urban Centers: Implications for Health.

LGB people may become less accessible to health professionals as technology reshapes LGB social connections. Though electronic forms of health promotion are effective, some interventions must be delivered in person. We use GIS to analyze characteristics (e.g., gender identity, depression, and performance involvement) shared by 114 gay, cisgender male drag performers. We used geographic ordinary least squares (OLS) regression to identify spatial relationships based on participant distance from urban centers. Participants lived between 1.73 km (1.07 mi) and 762.77 km (101.14 mi) from the nearest urban center (Mdn = 16.60 km; 10.31 mi). We found a positive correlation between distance from the nearest urban center and performance involvement (R = 0.19, p ≤ 0.05). Results indicate that drag performers are widely disbursed relative to urban centers and that drag involvement provides motivation to travel to gay bars. Health professionals with in person services may use drag shows and related performances to access LGB populations.

Decisional conflict among couples seeking specialty treatment for infertility in the USA: a longitudinal exploratory study.

STUDY QUESTION: What are couples' decisional conflicts around family-building approaches before and after seeking a specialty consultation for infertility? SUMMARY ANSWER: Decisional conflict is high among couples before an initial specialty consultation for infertility; on average, women resolved decisional conflict more quickly than men. WHAT IS KNOWN ALREADY: Couples have multiple options for addressing infertility, and decisional conflict may arise due to lack of information, uncertainty about options and potential risks or challenges to personal values. STUDY DESIGN, SIZE, DURATION: We conducted a total of 385 interviews and 405 surveys for this longitudinal, mixed-methods cohort study of 34 opposite-sex couples who sought a new reproductive specialty consultation (n = 68), who enrolled before the initial consultation and were followed over 12 months. PARTICIPANTS/MATERIALS, SETTING, METHODS: The in-depth, semi-structured interviews included questions about information gathering, deliberation and decision-making, and self-administered surveys included the Decisional Conflict Scale (DCS), at six time points over 12 months. A DCS total score of 25 is associated with implementing a decision, and higher scores indicate more decisional conflict. A systematic content analysis of interview transcripts identified major themes. Paired t tests identified differences in DCS between women and men within couples. Linear mixed models predicted changes in DCS over time, adjusting for sociodemographic and fertility-related factors. MAIN RESULTS AND THE ROLE OF CHANCE: The major qualitative themes were communication with partners, feeling supported and/or pressured in decision (s), changing decisions over time and ability to execute a desired decision. Average DCS scores were highest before the initial consultation. Within couples, men had significantly higher decisional conflict than women pre-consultation (48.9 versus 40.2, P = 0.037) and at 2 months (28.9 versus 22.1, P = 0.015), but differences at other time points were not significant. In adjusted models, predicted DCS scores declined over time, with women, on average, reaching the DCS threshold for implementing a decision at 2 months while for men it was not until 4 months. LIMITATIONS, REASONS FOR CAUTION: This is a convenience sample from a single center, and generalizability may be limited. WIDER IMPLICATIONS OF THE FINDINGS: Understanding how couples discuss and make decisions regarding family-building could improve the delivery of patient-centered infertility care. Our findings are the first to prospectively explore decisional conflict at multiple time points in both men and women; the observed gender differences underlie the importance of supporting both partners in clinical decision-making for infertility. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the National Institute of Child Health and Human Development under Grant [R21HD071332], the Research and Education Program Fund, of the Advancing a Healthier Wisconsin endowment at Medical College of Wisconsin, the National Research Service Award under Grant [T32 HP10030] and the use of REDCap for data collection from the National Center for Advancing Translational Sciences, National Institutes of Health under Grant through [8UL1TR000055]. The authors have no competing interests.

Equine herpesvirus-1 genotype did not significantly affect clinical signs and disease outcome in 65 horses diagnosed with equine herpesvirus-1 myeloencephalopathy.

The objective of this study was to determine if the genotype of equine herpesvirus-1 (EHV-1) impacted clinical disease and outcome of horses with laboratory confirmed equine herpesvirus myeloencephalopathy (EHM). Medical records from 65 horses diagnosed with EHM from 2011 to 2019 were reviewed for signalment, presence and severity of clinical signs (lethargy, fever, ataxia, urinary incontinence) and outcome. Horses were further grouped based on the EHV-1 genotype into neuropathic (D752) or non-neuropathic (N752) EHV-1 infection. Between the two EHV-1 genotype groups, age and sex distributions were similar, although breed distribution was different (Quarter Horses and Saddlebreds were overrepresented and Warmbloods were underrepresented in the EHV-1 D752 group compared to the EHV-1 N752 group; P = 0.009). Lethargy, fever, ataxia and outcome were not significantly different between the two EHV-1 genotype groups (P > 0.05). However, urinary incontinence was significantly more frequently reported in horses infected with the D752 genotype of EHV-1 (P=0.04). Contrary to previous studies, the present study showed no difference in frequency of genotype (D752 or N752) among 65 horses with EHM and, with the exception of urinary incontinence, no difference in clinical disease or outcome related to the EHV-1 genotype.

Sexual Dysfunction and Reproductive Concerns in Young Men Diagnosed With Testicular Cancer: An Observational Study.

INTRODUCTION: The survival rates for testicular cancer are excellent; still, there is a lack of knowledge regarding important survivorship issues, such as sexual dysfunction and reproductive concerns. AIM: The aim of this study was to investigate the prevalence and predictors of sexual dysfunction and reproductive concerns and the potential association between these issues in young men ∼2 years after a diagnosis of testicular cancer. METHODS: Data were collected from 111 men (response rate = 50%) diagnosed with testicular cancer at age 16-39. Patients were identified via the Swedish National Quality Registry for Testicular Cancer and approached with a survey, including standardized measures of sexual function, reproductive concerns, body image, and health-related quality of life. The survey was sent to participants approximately 2 years after their cancer diagnosis. Clinical variables were collected from the registry. Predictors were identified by multivariable linear regression analyses. MAIN OUTCOME MEASURES: The main outcomes were sexual function, assessed with the Patient-Reported Outcomes Measurement Information System Sexual Function and Satisfaction measure version 2.0, and reproductive concerns, assessed with the Reproductive Concerns After Cancer scale. RESULTS: Sexual dysfunction was reported by 26% of men, and a high level of reproductive concerns was reported by 28%. Lower satisfaction with sex life was associated with older age (ß = -0.41), negative body image (ß = -0.42), not having a partner (ß = 4.8), and dissatisfaction with sex life before cancer (ß = 8.31). Negative body image was associated with reproductive concerns in the dimensions of fertility potential (ß = 0.06), partner disclosure (ß = 0.08), and child's health (ß = 0.07), whereas having had fertility preservation predicted higher levels of concerns with regard to personal health (ß = 0.52) and achieving pregnancy (ß = 0.53). Clinical variables did not predict either sexual function or reproductive concerns. CLINICAL IMPLICATIONS: Our results show that the majority of young men diagnosed with testicular cancer do not report sexual dysfunction or reproductive concerns 2 years after diagnosis. A sizeable minority, however, does report dysfunction or reproductive concerns, which should be recognized in the follow-up care of this population. STRENGTHS & LIMITATIONS: A strength of the study is the use of high-quality registry data and validated instruments. The lack of Swedish norms for sexual function and reproductive concerns is a possible limitation. CONCLUSION: A subgroup of young men treated for testicular cancer report sexual dysfunction or reproductive concerns approximately 2 years after diagnosis. Factors associated with these issues seem to mainly be psychological, rather than medical, nature. Ljungman L, Eriksson LE, Flynn KE, et al. Sexual Dysfunction and Reproductive Concerns in Young Men Diagnosed With Testicular Cancer: An Observational Study. J Sex Med 2019;16:1049-1059.

Oceanic protists with different forms of acquired phototrophy display contrasting biogeographies and abundance.

This first comprehensive analysis of the global biogeography of marine protistan plankton with acquired phototrophy shows these mixotrophic organisms to be ubiquitous and abundant; however, their biogeography differs markedly between different functional groups. These mixotrophs, lacking a constitutive capacity for photosynthesis (i.e. non-constitutive mixotrophs, NCMs), acquire their phototrophic potential through either integration of prey-plastids or through endosymbiotic associations with photosynthetic microbes. Analysis of field data reveals that 40-60% of plankton traditionally labelled as (non-phototrophic) microzooplankton are actually NCMs, employing acquired phototrophy in addition to phagotrophy. Specialist NCMs acquire chloroplasts or endosymbionts from specific prey, while generalist NCMs obtain chloroplasts from a variety of prey. These contrasting functional types of NCMs exhibit distinct seasonal and spatial global distribution patterns. Mixotrophs reliant on 'stolen' chloroplasts, controlled by prey diversity and abundance, dominate in high-biomass areas. Mixotrophs harbouring intact symbionts are present in all waters and dominate particularly in oligotrophic open ocean systems. The contrasting temporal and spatial patterns of distribution of different mixotroph functional types across the oceanic provinces, as revealed in this study, challenges traditional interpretations of marine food web structures. Mixotrophs with acquired phototrophy (NCMs) warrant greater recognition in marine research.

Plasma l-citrulline concentrations in l-arginine-supplemented healthy dogs.

INTRODUCTION: To determine whether oral l-arginine increases plasma [l-citrulline] in dogs. ANIMALS: Eleven healthy staff-owned dogs were used in this study. MATERIALS AND METHODS: Dogs (n = 3) were given l-arginine (50mg/kg PO q8h) for 7 days, and plasma [l-arginine] and [l-citrulline] were analyzed by high performance liquid chromatography at baseline (BL), steady state trough, and 0.5, 1, 1.5, 2, 4, 6, and 8 h after final dosing on day 7. Eleven dogs were then treated with 100mg/kg l-arginine PO q8h for 7 days, and [l-arginine] and [l-citrulline] were measured at BL, steady state trough, and at peak 4 hrs after dosing (T4 hrs). RESULTS: - Plasma [l-arginine] and [l-citrulline] peaked at T4 hrs on the 50mg/kg dosage. Target outcome, modeled after human study results, of a doubling of [l-arginine] and a 25-30% increase in [l-citrulline] from BL were not reached. After the 100mg/kg dosage, plasma [l-arginine] increased from a BL median of 160.1 µM (range, 100.2-231.4 µM) to a peak of 417.4 µM (206.5-807.3 µM) at T4 hrs, and plasma [l-citrulline] increased from a BL median of 87.8 µM (59.1-117.1 µM) to peak of 102.2 µM (47.4-192.6 µM) at T4 hrs. Ten of eleven dogs showed a doubling of plasma [l-arginine] and 4/11 dogs achieved 25-30% or greater increases in plasma [l-citrulline]. No adverse effects on heart rate or blood pressure were noted. CONCLUSIONS: - Oral l-arginine dosage of 100mg/kg q8h doubles plasma [l-arginine] in healthy dogs, but conversion to l-citrulline is quite variable. Further evaluation of this dosage regimen in dogs with pulmonary hypertension is warranted.

Can we agree on patient-reported outcome measures for assessing hematopoietic cell transplantation patients? A study from the CIBMTR and BMT CTN.

Much research into the impact of hematopoietic cell transplantation (HCT) on recipients' symptoms, functioning and health-related quality of life uses diverse patient-reported outcome (PRO) measures. Robust conclusions regarding PROs in HCT patients are constrained by methodological issues, including the use of multiple different and noncomparable assessment measures. We reviewed 114 publications addressing PROs in HCT patients. Although three multi-item measures were most frequently used (FACT-BMT, n=28; European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-C30, n=26; and SF-36, n=26), 25 additional measures were used in more than one study. Another 50 measures were used in single studies. Over 50% of studies used more than one measure. We recommend that the field agrees upon a set of measures to address the core domains important to patients, to reduce heterogeneity and allow comparisons across studies and between different populations. Measures should be available in a free and easily accessible manner internationally. We discuss the relative benefits of the National Institutes of Health-supported Patient-Reported Outcomes Measurement Information System (PROMIS) system to achieve these goals. To further address these issues, the Blood and Marrow Transplant Clinical Trials Network has recently created a task force to implement PROMIS measures alongside traditional PRO measures in future clinical trials. Robust comparisons between measures in this setting may allow for the development of a standard for HCT patients.