RESUMO
The previous works on simulated broths are continued and developed for Propionibacterium shermanii broths. The obtained results indicated the considerable increase of kLa in presence of n-dodecane as oxygen-vector and the existence of a certain value of hydrocarbon concentration that corresponds to the maximum mass transfer rate of oxygen. The magnitude of the positive effect of the oxygen-vector strongly depends on operational conditions of the bioreactor, on broth characteristics and on P. shermanii concentration.
Assuntos
Reatores Biológicos/microbiologia , Modelos Teóricos , Oxigênio/metabolismo , Propionibacterium/crescimento & desenvolvimento , Pressão ParcialRESUMO
Pertraction (permeation through liquid membranes) is one of the new techniques applied for separation and advanced purification of bioactive compounds. The study on the facilitated pertraction of vitamin C with Amberlite LA-2 underlined the significant influences of the pH-gradient between the aqueous phases, carrier concentration and mixing intensity. Thus, for reaching the maximum efficiency of vitamin C pertraction, the pH-value of the initial aqueous phase below 2, the pH-value of the final aqueous solution greater than 11 and the Amberlite LA-2 concentration in solvent layer over 80 g/l are required.