Effect of temperature on frequencies of spots in Drosophila wing-spot assay.

The effect of temperature on spontaneous mutation frequencies was studied in the Drosophila somatic mutation and recombination test. Transdihybrid mwh +/+ flr3 larvae were grown at various temperatures (15, 18, 20, 22, 25, 27, and 29 degrees C) in the absence of any chemical mutagen. Wings of surviving adults were removed and scored for presence of small single spots, large single spots, and twin spots. No significant effect of temperature was found on mean frequency per wing of twin spots. Similarly, no significant effect of temperature was found for large singles within the temperature range of 18-29 degrees C; however, at 15 degrees C, the mean frequency per wing of large spots was significantly elevated. A significant quadratic relationship was found between mean frequency of small spots and temperature. Frequency of small spots per wing was minimized within the temperature range of 20-27 degrees C and increased at higher and lower temperatures. Maximum frequency of small spots per wing was observed at 15 degrees C. The qualitative nature of the small single spots induced at high and low temperatures is unclear; they may represent slow-growing segmentally aneuploid (deleted) cells or possibly even monosomic cells. Both heat and cold were found to be mutagenic in the Drosophila wing-spot assay. However, the mutagenic potency associated with temperature was much less than that of most chemical mutagens, and no significant effect of temperature was observed in the range of 20-27 degrees C.

Effect of innovation characteristics on pharmacists' use of written patient medication information.

There is increasing pressure on pharmacists to provide written medication information in patients. Unfortunately, recent evidence indicates that this practice has not yet been extensively adopted. Pharmacists' reluctance can be explained partially by Diffusion of Innovations Theory which states that the characteristics of an innovation (i.e. relative advantage, compatibility with needs, experience and values, complexity and trialability) can affect its rate of adoption within a community. This theory was tested in a group of Michigan community pharmacists who recently purchased medication information sheets developed by the Michigan Pharmacists Association. Results were compared with a randomly selected sample of similar pharmacists who had not yet purchased the kit. Study findings support the theory that the adoption of an innovation (i.e. use of written information) is affected by the adopters' perception of certain innovation characteristics. The most influential factor in the adoption of the written information sheets was the extent that they were compatible with the pharmacists' business needs, professional values, and past experiences with providing written information to patients. The most important deterrent to adoption of the sheets appeared to be the perceived complexity in implementation at the practice site.