Saline-infused radiofrequency ablation: A review on the key factors for a safe and reliable tumour treatment.

Radiofrequency ablation (RFA) combined with saline infusion into tissue is a promising technique to ablate larger tumours. Nevertheless, the application of saline-infused RFA remains at clinical trials due to the contradictory findings as a result of the inconsistencies in experimental procedures. These inconsistencies not only magnify the number of factors to consider during the treatment, but also obscure the understanding of the role of saline in enlarging the coagulation zone. Consequently, this can result in major complications, which includes unwanted thermal damages to adjacent tissues and also incomplete ablation of the tumour. This review aims to identify the key factors of saline responsible for enlarging the coagulation zone during saline-infused RFA, and provide a proper understanding on their effects that is supported with findings from computational studies to ensure a safe and reliable cancer treatment.

Partially-covered fractal induced turbulence on fins thermal dissipation.

The impacts of partially-covered fractal grids induced turbulence on the forced convective heat transfer across plate-fin heat sink at Reynolds number ReDh = 22.0 × 103 were numerically and experimentally investigated. Results showed that partially covered grids rendered a higher thermal dissipation performance, with partially-covered square fractal grid (PCSFG) registering an outstanding increase of 43% in Nusselt number relative to the no grid configuration. The analyzation via an in-house developed single particle tracking velocimetry (SPTV) system displayed the findings of unique "Turbulence Annulus" formation, which provided a small degree of predictivity in the periodic annulus oscillations. Further assessments on PCSFG revealed the preferred inter-fin flow dynamics of (i) high flow velocity, (ii) strong turbulence intensity, (iii) vigorous flow fluctuations, (iv) small turbulence length scale, and (v) heightened decelerated flow events. These features stemmed from the coupling effects of multilength-scale fractal bar thicknesses in generating a veracity of eddy sizes, and a vertical segmentation producing heightened mass flow rate while inducing favourable wake-flow structures to penetrate inter-fin regions. Teeming effects of such energetic eddies within plate-fin array unveiled a powerful vortex shedding effect, with PCSFG achieving fluctuation frequency f = 18.5 Hz close to an optimal magnitude. The coaction of such traits limits the growth of fin boundary layers, providing superior thermal transfer capabilities which benefits the community in developing for higher efficiency heat transfer systems.

SPTV sheds light on flow dynamics of fractal-induced turbulence over a plate-fin array forced convection.

A 3D stationary particle tracking velocimetry (SPTV) with a unique recursive corrective algorithm has been successfully established to detect the instantaneous regional fluid flow characteristics. The veracity of SPTV is corroborated by conducting actual displacement measurement validation, which gives a maximum percentage deviation of about 0.8%. This supports the accuracy of the current SPTV system in 3D position detection. More importantly, the SPTV detected velocity fluctuations are highly repeatable. In this study, SPTV is proven to be able to express the nature of chaotic fractal grid-induced regional turbulence, namely: the high turbulence intensity attributed to multilength-scale wake interactions, the Kolmogorov's -5/3 law decay, vortex shedding, and the Gaussian flow undulations immediately leeward of the grid followed by non-Gaussian behaviour further downstream. Moreover, by comparing the flow fields between control no-grid and fractal grid-generated turbulence of a plate-fin array, SPTV reveals vigorous turbulence intensity, smaller regional integral-length-scale, and energetic vortex shedding at higher frequency for the latter, particularly between fins. Thereupon, it allows the unravelling of detailed thermofluid interplays of plate-fin heat sink heat transfer augmentation. The novelty of SPTV lies in its simplicity, use of low-cost off-the-shelf components, and most remarkably, low computational complexity in detecting fundamental characteristics of turbulent fluid flow.

Fractal grid-induced turbulence strength characterization via piezoelectric thin-film flapping velocimetry.

The centerline streamwise and cross-sectional (x/Dh = 0.425) turbulence characteristics of a 2D planar space-filling square-fractal-grid (SFG) composed of self-similar patterns superimposed at multiple length-scales is experimentally unveiled via piezoelectric thin-film flapping velocimetry (PTFV). The fluid-structure-interaction between a flexible piezoelectric thin-film and SFG-generated turbulent flow at ReDh = 4.1 × 104 is investigated by analysis of the thin-film's mechanical response. Measurements of the thin-film-tip deflection δ and induced voltage V demonstrate increasing flow fluctuation strength in the turbulence generation region, followed by rapid decay further downstream of the SFG. Interestingly, SFG-induced turbulence enables the generation of maximum centerline thin-film's response (Vrms, δrms) and millinewton turbulence-forcing (turbulence-induced excitation force acting on the thin-film) Frms which are respectively, 7× and 2× larger than the classical square-regular-grid of similar blockage ratio. The low frequency, large-scale energy-containing eddies at SFG's central opening plays a critical role in driving the thin-film vibration. Most importantly, the SFG-generated turbulence at (y/T = 0.106, z/T = 0.125) away from the centerline allows equivalent mechanical characteristics of turbulence generation and decay, with peak of 1.9× nearer from grid. In short, PTFV provides a unique expression of the SFG-generated turbulence, of which, the equivalent turbulence length-scale and induced-forcing deduced could aid in deciphering the flow dynamics for effective turbulence management.

Fractal-induced 2D flexible net undulation.

A net immersed in fractal-induced turbulence exhibit a transient time-varying deformation. The anisotropic, inhomogeneous square fractal grid (SFG) generated flow interacts with the flexible net to manifest as visible cross-sectional undulations. We hypothesize that the net's response may provide a surrogate in expressing local turbulent strength. This is analysed as root-mean-squared velocity fluctuations in the net, displaying intensity patterns dependent on the grid conformation and grid-net separation. The net's fluctuation strength is found to increase closer to the turbulator with higher thickness ratio while presenting stronger fluctuations compared to regular-square-grid (RSG) of equivalent blockage-ratio, σ. Our findings demonstrate a novel application where 3D-reconstruction of submerged nets is used to experimentally contrast the turbulence generated by RSG and multilength scale SFGs across the channel cross-section. The net's response shows the unique turbulence developed from SFGs can induce 9 × higher average excitation to a net when compared against RSG of similar σ.

Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction.

Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell-matrix and cell-cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell-cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions.

Coupling bending and shear effects on liposome deformation.

Cell membrane deformation induced by external mechanical stimuli has been studied extensively over the past three decades. The present study focuses on the coupling of in-plane shear H and out-of-plane bending B of liposome membrane and its influences on the deformation of a single vesicle subjected to (i) external compressive load via two parallel platens and (ii) contact forces caused by a rigid substrate. Our results show that the increase of membrane resultant stress in both loading configurations causes the liposome to become more rigid and the degree of vesicle deformation decreases when the in-plane shearing effect is dominant. A theoretical approach is developed to facilitate cell membrane characterization under different biomechanical stimuli.

Human red blood cells deformed under thermal fluid flow.

The flow-induced mechanical deformation of a human red blood cell (RBC) during thermal transition between room temperature and 42.0 degrees C is interrogated by laser tweezer experiments. Based on the experimental geometry of the deformed RBC, the surface stresses are determined with the aid of computational fluid dynamics simulation. It is found that the RBC is more deformable while heating through 37.0 degrees C to 42.0 degrees C, especially at a higher flow velocity due to a thermal-fluid effect. More importantly, the degree of RBC deformation is irreversible and becomes softer, and finally reaches a plateau (at a uniform flow velocity U > 60 microm s(-1)) after the heat treatment, which is similar to a strain-hardening dominated process. In addition, computational simulated stress is found to be dependent on the progression of thermotropic phase transition. Overall, the current study provides new insights into the highly coupled temperature and hydrodynamic effects on the biomechanical properties of human erythrocyte in a model hydrodynamic flow system.

Shape recovery of an optically trapped vesicle: effect of flow velocity and temperature.

A new biophysical approach based on optical tweezers is developed to measure the time-dependent shape transformation and recovery of a single liposome, which is induced by the sudden stop of a moving liposome from various flow velocities at constant temperature. A simple viscoelastic model has been applied to correlate the temporal geometric parameter of the deformed liposome with a characteristic time constant, i.e., the ratio of membrane viscosity to elasticity. Our results show that membrane viscosity becomes dominant in governing the shape recovery rate when sample temperature goes beyond the main phase transition temperature of the phospholipid bilayer. More importantly, flow speed and vesicle size are demonstrated as key physical determinants for the shape recovery of liposome.

Contact deformation of liposome in the presence of osmosis.

The role of osmotic pressure on the geometry of adherent liposome remains an intricate question in the mechanics of supramolecular structures. In this study, confocal reflection interference contrast microscopy in combination with cross-polarized microscopy was applied to probe the geometry of deformed liposome on fused silica substrates through the determination of a vesicle-substrate separation profile. In parallel, a theoretical model which describes the large deformation of the lipid bilayer membrane under both out-plane bending and in-plane shear forces is developed. Then, the global deformation geometry of the adherent liposome is rigorously compared with our experimental data. It is shown that the adhesion contact area increases in dimension, the liposome volume decreases, and the vesicle height decreases under the reduced osmotic pressure. The coupling of experimental data and a modified theoretical framework of the adherent liposome provides a more explicit result in comparison with previous studies and demonstrates the possibility of modeling the change of liposome mechanics under the influence of osmosis.

Thermal effect on a viscously deformed liposome in a laser trap.

The viscous drag and mechanical deformation of a single vesicle under hydrodynamics flow during the phase transition of a lipid bilayer is determined by optical tweezers experiments with the aid of computational fluid dynamics simulations. Based on the experimental geometry of the vesicle under hydrodynamics flow, the surface stresses and drag force are numerically calculated. It is found that the vesicle is less rigid and the viscous drag force of the vesicle decreases with the increase of temperature at low Reynolds number flow during sample heating. Interestingly, these mechanical properties are reversible and depend strongly on the liposome's thermotropic phase transition temperature. Overall, this study provides new insights into highly coupled thermal and hydrodynamics effects on the biomechanical properties of model membrane vesicle in physiological flow systems.