RESUMO
OBJECTIVE: Central obesity and sub-clinical inflammation increase metabolic risk, this study examined the intracellular inflammatory pathways in adipose tissue (AT) that contribute to this risk. DESIGN AND METHODS: This study therefore addressed the influence of NFκB and JNK activation in human abdominal subcutaneous (AbdSc) and omental (Om) AT, the effect of adiposity, T2DM status and the role of TNFα in vitro, using molecular biology techniques. RESULTS: Our data showed NFκB activity is increased in Om AT versus AbdSc AT (P<0.01), which was reversed with respect to depot specific activation of JNK (P<0.01). However, T2DM status appeared to preferentially activate NFκB (P<0.001) over JNK. Furthermore, in vitro studies showed recombinant human (rh) TNFα treated AbdSc adipocytes increased NFκB activity over time (2-48 h, P<0.05) whilst JNK activity reduced (2 h, 4 h, P<0.05); inhibitor studies supported a preferential role for NFκB as a modulator of TNFα secretion. CONCLUSIONS: These studies suggest distinct changes in NFκB and JNK activation, dependent upon AT depot, adiposity and T2DM status, with in vitro use of rh TNFα leading to activation of NFκB. Consequently NFκB appears to play a central role in inflammatory mediated metabolic disease over JNK, highlighting NFκB as a potential key target for therapeutic intervention.
Assuntos
Tecido Adiposo/metabolismo , Adiposidade/fisiologia , Diabetes Mellitus Tipo 2/genética , NF-kappa B/fisiologia , Paniculite/genética , Fator de Necrose Tumoral alfa/fisiologia , Tecido Adiposo/imunologia , Tecido Adiposo/patologia , Adulto , Idoso , Células Cultivadas , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patologia , Feminino , Humanos , Inflamação/genética , Inflamação/metabolismo , Masculino , Pessoa de Meia-Idade , Proteína Quinase 8 Ativada por Mitógeno/genética , Proteína Quinase 8 Ativada por Mitógeno/metabolismo , Proteína Quinase 9 Ativada por Mitógeno/genética , Proteína Quinase 9 Ativada por Mitógeno/metabolismo , Paniculite/metabolismo , Proteínas Recombinantes/farmacologia , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
Parents, librarians and educators alike are invested in children learning to read. The library storytime provides a unique opportunity to introduce skills essential to pre-literacy development. This article reviews the literature on school-aged children and applies these findings as a basis for activities appropriate for pre-readers. Important areas for the development of pre-literacy are identified and explained, including alphabet knowledge, concepts about print, book handling skills, phonological awareness and expressive vocabulary. Specific activities using children's literature for each of these areas are provided.
RESUMO
The main hypotheses addressed in the research were (1) whether imprecision in the phonological representations of lexical items underlies the impaired expressive naming abilities of disabled readers, and (2) whether weak verbal memory might mediate the relationship between naming and reading skills. From samples of 93 first graders and 67 fourth graders, extreme groups of good and poor readers were identified and compared on measures of receptive vocabulary, expressive naming, acceptability judgments for variants of object names, imitation and correction of naming errors by another speaker, pseudoword repetition, and long-term memory. Performance was generally better by older than younger students and by good than poor readers at each age, with little interaction between grade and reader group. The results indicated that for both good and poor readers, imprecise phonological knowledge, especially about long words, contributed to children's difficulties on all naming tasks. Memory differences, however, appeared to play only a minor role in explaining the strong association between naming and reading.
