[Evaluation of Sysmex UF-1000i® flow cytometer as a screening method for asymptomatic bacteriuria and detection of Group B Streptococcus in pregnancy]. / Evaluación del citómetro de flujo Sysmex UF-1000i® como método de cribado de bacteriuria asintomática y estreptococo grupo B en la mujer embarazada.

OBJECTIVE: Nowadays, the use of flow-cytometry for the screening of urine samples is extended, but appropriate cut-off points for each population group are yet to be established. The objective of this study was to evaluate the Sysmex UF-1000i® cytometer as a screening method for detection of asymptomatic bacteriuria (AB) and Group B Streptococcus (GBS) in pregnant women. METHODS: Urine samples obtained during pregnancy between January-July 2019 were both processed with the Sysmex UF-1000i® and also cultured. Demographic data, flow-cytometry parameters and the result of the urine culture were collected. To assess the performance of the flow-cytometer for detection of AB and GBS, receiver operating characteristic (ROC) curves for the BACT/µL variable were applied. RESULTS: A total of 33,687 urine samples were received, among which 1,443 (4.3%) belonged to pregnant women. Urine culture was positive in 82 (5.7%) samples, 1,295 (89.7%) were negative and 66 (4.6%) were considered contaminated. GBS was isolated in 69 (4.8%) patients and 46 (66.7%) with a microbial count below 10E4 CFU/mL. For AB detection, the cut-off point of 550 BACT/µL yielded a sensitivity of 91.5%, a negative predictive value of 99.3% and could avoid culturing 74.1% of the samples. No cut-off value could be established for GBS detection. CONCLUSIONS: Although the Sysmex UF-1000i® system is a valid screening method for the AB detection in pregnant population, it is not useful for the identification of GBS bacteriuria in our area. Therefore, the conventional urine culture is still required during pregnancy.

La fiebre intraparto como factor predictivo de parto distócico y resultado neonatal adverso / Intrapartum fever as a predictive factor of dystocic delivery and adverse neonatal outcome

La fiebre intraparto tiene una prevalencia del 10-34%. Se asocia a anestesia epidural, parto prolongado, aumento de partos instrumentales y cesáreas. Estudiamos a 696 mujeres con fiebre intraparto de las 2431 que parieron en nuestro hospital en el año 2011. Las dividimos en 2 grupos en función de que su temperatura sea mayor (o igual) o menor de 38 °C. Analizamos las características del parto y del recién nacido en ambos grupos. La incidencia de fiebre ha sido del 17,4%. En el grupo de fiebre intraparto observamos un trabajo de parto significativamente más largo, el doble de partos instrumentales y 3 veces más de cesáreas. No se observaron diferencias en el test de Apgar ni en el pH de cordón de los recién nacidos

Intrapartum fever has a prevalence of 10-34%. It is associated with epidural analgesia, prolonged labor and an increased incidence of assisted vaginal delivery and cesarean delivery. Of a total of 2431 women who delivered in our hospital in 2011, we studied 696 women with intrapartum fever. They were divided into two groups according to whether their temperature was 38 °C or higher, or was lower than 38 °C. We analyzed the characteristics of the delivery and the newborn in both groups. The incidence of fever was 17.4%. In the intrapartum fever group, labor was significantly longer than in the group without fever, and there were twice as many assisted vaginal deliveries and three times as many cesarean deliveries. There were no differences in the Apgar test or umbilical cord Ph