Expansion of the genus Imleria in North America to include Imleria floridana, sp. nov., and Imleria pallida, comb. nov.

Imleria is a small genus of Boletaceae found primarily in the Northern Hemisphere characterized by the light to dark brown hymenophore that stains blue. In Florida, specimens of Imleria were collected that resembled I. badia, a species known primarily from northeastern North America and Europe. Five nuclear loci of these Florida specimens were sequenced and phylogenetically analyzed. A nuc rDNA internal transcribed spacer ITS1-5.8S-ITS2 (ITS) phylogeny of Imleria was generated using primarily uncultured environmental sequences to confirm ectomycorrhizal associations. Based on morphological and molecular data, we describe a new species, Imleria floridana. Results from these analyses also support the new combination Imleria pallida. A lectotype of I. pallida is also established from Charles H. Peck's original material. We discuss the distinguishing characters and species of Imleria found in North America and provide a key to the known worldwide species of Imleria.

Typifications for Galactia purshii and G. volubilis (Fabaceae).

The pervasive difficulties encountered with studying Galactia in the eastern USA necessitate additional typifications to stabilize the taxonomy. Galactia purshii is lectotypified here as the earliest available but overlooked name for a fairly common species of the Atlantic Coast and peninsular Florida. Galactia volubilis is epitypified here since the characterization of the species has been limited by its lectotype being an illustration. A lectotype is designated for Galactia pilosa var. angustifolia, a form with narrow leaves here considered synonymous with G. volubilis.

Low-copy nuclear primers and ycf1 primers in Cactaceae.

PREMISE OF THE STUDY: To increase the number of variable regions available for phylogenetic study in the Cactaceae, primers were developed for a portion of the plastid ycf1 gene and intron-spanning regions of two low-copy nuclear genes (isi1, nhx1). • METHODS AND RESULTS: Primers were tested on several families within Caryophyllales, focusing on the Cactaceae. Gel electrophoresis indicated positive amplification in most samples. Sequences of these three regions (isi1, nhx1, ycf1) from Harrisia exhibited variation similar to or greater than two plastid regions (atpB-rbcL intergenic spacer and rpl16 intron). • CONCLUSIONS: The isi, nhx, and ycf1 primers amplify phylogenetically useful information applicable to the Cactaceae and other families in the Caryophyllales.